LOCUS       sequence-227283-       15573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use as a positive control for translation of the Rluc gene in 
            the transfected cells and negative control for replication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15573)
  AUTHORS   Alcaraz-Estrada SL, Manzano MI, Del Angel RM, Levis R, Padmanabhan R
  TITLE     Construction of a dengue virus type 4 reporter replicon and analysis
            of temperature-sensitive mutations in non-structural proteins 3 and 
            5.
  JOURNAL   J Gen Virol. 2010 Nov;91(Pt 11):2713-8. doi: 10.1099/vir.0.024083-0.
            Epub 2010 Jul 14.
  PUBMED    20631089
REFERENCE   2  (bases 1 to 15573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol."; date: "2010-11"; volume: "91(Pt 11):2713-8. doi"; pages: " 
            10.1099/vir.0.024083-0. Epub 2010 Jul 14"
COMMENT     Sequence Label: DENV4 Rluc Reporter GND mutant Replicon
FEATURES             Location/Qualifiers
     source          1..15573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             174..1109
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYTKSFVERVLKNEQ"
     misc_feature    1113..1685
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1280..1297)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1507..1526
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(10048..10068)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10048..10066)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10087..10103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10087..10103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10100..10122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10111..10127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10135..10141,10142..10159,10160..10165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10180..10201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10318..10335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10489..11077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10569..10588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11248..12039,12040..12108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11871..11890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12109..12213)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12240..13582
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13203..13250)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13623..13641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13679..13701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13801..13820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        13841..14122
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             14123..14797
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(14900..15355)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15037..15058)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15249..15268
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15481..15503
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15495..15512
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15496..15512
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15522..15541
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15522..15540
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        join(15556..15573,1)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15556..15573
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agttgttagt ctgtgtggac cgacaaggac agttccagat cggaagcttg cttaacacag
       61 ttctaacagt ttgtttgaat agagagcaga tctctggaaa aatgaaccaa cgaaaaaagg
      121 tggttagacc acctttcaat atgctgaaac gcgagagaaa ccgcgtgtcg actatggctt
      181 ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc
      241 gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg
      301 ccgagaacgc cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg
      361 tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta
      421 agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg
      481 cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg
      541 cttgtctggc ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg
      601 ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata
      661 tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg
      721 agaccatgct cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc
      781 tggagccatt caaggagaag ggcgaggtta gacggcctac cctctcttgg cctcgcgaga
      841 tccctctcgt taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct
      901 accttcgggc cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt
      961 ccaacgctat tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg
     1021 gcctccactt cagccaggag gacgctccag atgaaatggg taagtacacc aagagcttcg
     1081 tggagcgcgt gctgaagaac gagcagtgac cgcccctctc cctccccccc ccctaacgtt
     1141 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1201 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1261 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1321 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     1381 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     1441 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     1501 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     1561 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     1621 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     1681 taataccatg gggttcttag tgttgtggat tggcacgaac tcaaggaaca cttcaatggc
     1741 tatgacgtgc atagctgttg gaggaatcac tctgtttctg ggcttcacag ttcaagcaga
     1801 catgggttgt gtggtgtcat ggagtgggaa agaattgaag tgtggaagcg gaatttttgt
     1861 ggttgacaac gtgcacactt ggacagaaca gtacaaattt caaccagagt ccccagcgag
     1921 actagcgtct gcaatattaa atgcccacaa agatggggtc tgtggaatta gatcaaccac
     1981 gaggctggaa aatgtcatgt ggaagcaaat aaccaacgag ctaaactatg ttctctggga
     2041 aggaggacat gacctcactg tagtggctgg ggatgtgaag ggggtgttga ccaaaggcaa
     2101 gagagcactc acacccccag tgagtgatct gaaatattca tggaagacat ggggaaaagc
     2161 aaaaatcttc accccagaag caagaaacag cacattttta atagacggac cagacacctc
     2221 tgaatgcccc aatgaacgaa gagcatggaa ctctcttgag gtggaagact atggatttgg
     2281 catgttcacg accaacatat ggatgaaatt ccgagaagga agttcagaag tgtgtgacca
     2341 caggttaatg tcagctgcaa ttaaagatca gaaagctgtg catgctgaca tgggttattg
     2401 gatagagagc tcaaaaaacc agacctggca gatagagaaa gcatctctta ttgaagtgaa
     2461 aacatgtctg tggcccaaga cccacacact gtggagcaat ggagtgctgg aaagccagat
     2521 gctcattcca aaatcatatg cgggcccttt ttcacagcac aattaccgcc agggctatgc
     2581 cacgcaaacc gtgggcccat ggcacttagg caaattagag atagactttg gagaatgccc
     2641 cggaacaaca gtcacaattc aggaggattg tgaccataga ggcccatctt tgaggaccac
     2701 cactgcatct ggaaaactag tcacgcaatg gtgctgccgc tcctgcacga tgcctccctt
     2761 aaggttcttg ggagaagatg ggtgctggta tgggatggag attaggccct tgagtgaaaa
     2821 agaagagaac atggtcaaat cacaggtgac ggccggacag ggcacatcag aaactttttc
     2881 tatgggtctg ttgtgcctga ccttgtttgt ggaagaatgc ttgaggagaa gagtcactag
     2941 gaaacacatg atattagttg tggtgatcac tctttgtgct atcatcctgg gaggcctcac
     3001 atggatggac ttactacgag ccctcatcat gttgggggac actatgtctg gtagaatagg
     3061 aggacagatc cacctagcca tcatggcagt gttcaagatg tcaccaggat acgtgctggg
     3121 tgtgttttta aggaaactca cttcaagaga gacagcacta atggtaatag gaatggccat
     3181 gacaacggtg ctttcaattc cacatgacct tatggaactc attgatggaa tatcactggg
     3241 actaattttg ctaaaaatag taacacagtt tgacaacacc caagtgggaa ccttagctct
     3301 ttccttgact ttcataagat caacaatgcc attggtcatg gcttggagga ccattatggc
     3361 tgtgttgttt gtggtcacac tcattccttt gtgcaggaca agctgtcttc aaaaacagtc
     3421 tcattgggta gaaataacag cactcatcct aggagcccaa gctctgccag tgtacctaat
     3481 gactcttatg aaaggagcct caagaagatc ttggcctctt aacgagggca taatggctgt
     3541 gggtttggtt agtctcttag gaagcgctct tttaaagaat gatgtccctt tagctggccc
     3601 aatggtggca ggaggcttac ttctggcggc ttacgtgatg agtggtagct cagcagatct
     3661 gtcactagag aaggccgcca atgtgcagtg ggatgaaatg gcagacataa caggctcaag
     3721 cccaatcata gaagtgaagc aggatgaaga tggctctttc tccatacggg acgtcgagga
     3781 aaccaatatg ataacccttt tggtgaaact ggcactgata acagtgtcag gtctctaccc
     3841 cttggcaatt ccagtcacaa tgaccttatg gtacatgtgg caagtgaaaa cacaaagatc
     3901 aggagccctg tgggacgtcc cctcacccgc tgccactcaa aaagccgcac tgtctgaagg
     3961 agtgtacagg atcatgcaaa gagggttatt cgggaaaact caggttggag tagggataca
     4021 catggaaggt gtatttcaca caatgtggca tgtaacaaga ggatcagtga tctgccacga
     4081 gactgggaga ttggagccat cttgggctga cgtcaggaat gacatgatat catacggtgg
     4141 gggatggagg cttggagaca aatgggacaa agaagaagac gttcaggtcc tcgccataga
     4201 accaggaaaa aatcctaaac atgtccaaac gaaacctggc cttttcaaga ccctaactgg
     4261 agaaattgga gcagtaacat tagatttcaa acccggaacg tctggttctc ccatcatcaa
     4321 caggaaagga aaagtcatcg gactctatgg aaatggagta gttaccaaat caggtgatta
     4381 cgtcagtgcc ataacgcaag ccgaaagaat tggagagcca gattatgaag tggatgagga
     4441 catttttcga aagaaaagat taactataat ggacttacac cccggagctg gaaagacaaa
     4501 aagaattctt ccatcaatag tgagagaagc cttaaaaagg aggctgcgaa ctttgatttt
     4561 ggctcccacg agagtggtgg cggccgagat ggaagaggcc ctacgtggac tgccaatccg
     4621 ttatcagacc ccagctgtga aatcagaaca cacaggaaga gagattgtag acctcatgtg
     4681 tcatgcaacc ttcacaacaa gacttttgtc atcaaccagg gttccaaatt acaaccttat
     4741 agtgatggat gaagcacatt tcaccgatcc ttctagtgtc gcggctagag gatacatctc
     4801 gaccagggtg gaaatgggag aggcagcagc catcttcatg accgcaaccc ctcccggagc
     4861 gacagatccc tttccccaga gcaacagccc aatagaagac atcgagaggg aaattccgga
     4921 aaggtcatgg aacacagggt tcgactggat aacagactac caagggaaaa ctgtgtggtt
     4981 tgttcccagc ataaaagctg gaaatgacat tgcaaattgt ttgagaaagt cgggaaagaa
     5041 agttatccag ttgagtagga aaacctttga tacagagtat ccaaaaacga aactcacgga
     5101 ctgggacttt gtggtcacta cagacatatc tgaaatgggg gccaatttta gagctgggag
     5161 agtgatagac cctagaagat gcctcaagcc agttatccta acagatgggc cagagagagt
     5221 cattttagca ggtcctattc cagtgactcc agcaagcgct gctcagagaa gagggcgaat
     5281 aggaaggaac ccagcacaag aagacgacca atacgttttc tccggagacc cactaaaaaa
     5341 tgatgaagat catgcccact ggacagaagc aaagatgctg cttgacaata tctacacccc
     5401 agaagggatc attccaacat tgtttggtcc ggaaagggaa aaaacccaag ccattgatgg
     5461 agagtttcgc ctcagagggg aacaaaggaa gacttttgtg gaattaatga ggagaggaga
     5521 ccttccggtg tggctgagct ataaggtagc ttctgctggc atttcttaca aagatcggga
     5581 atggtgcttc acaggggaaa gaaataacca aattttagaa gaaaacatgg aggttgaaat
     5641 ttggactaga gagggagaaa agaaaaagct aaggccaaga tggttagatg cacgtgtata
     5701 cgctgacccc atggctttga aggatttcaa ggagtttgcc agtggaagga agagtataac
     5761 tctcgacatc ctaacagaga ttgccagttt gccaacttac ctttcctcta gggccaagct
     5821 cgcccttgat aacatagtca tgctccacac aacagaaaga ggagggaggg cctatcaaca
     5881 cgccctgaac gaacttccgg agtcactgga aacactcatg cttgtagctt tactaggtgc
     5941 tatgacagca ggtattttcc tgtttttcat gcaagggaaa ggaataggga aattgtcaat
     6001 gggtttgata accattgcgg tggctagtgg cttgctctgg gtagcagaaa ttcaacccca
     6061 gtggatagcg gcctcaatca tactagagtt ttttctcatg gtactgttga taccagaacc
     6121 agaaaaacaa aggaccccac aagacaatca attgatctac gtcatattga ccattctcac
     6181 catcattggt ctaatagcag ccaacgagat ggggctgatt gaaaaaacaa aaacggattt
     6241 tgggttttac caggtaaaaa cagaaaccac catcctcgat gtggacttga gaccagcttc
     6301 agcatggacg ctttatgcag tagccaccac aattctgact cccatgctga gacacaccat
     6361 agaaaacacg tcggccaacc tatctctagc agccattgcc aaccaggcag ccgtcctaat
     6421 ggggcttgga aaaggatggc cgctccacag aatggacctc ggtgtgccgc tgttagcaat
     6481 gggatgctat tctcaagtga acccaacaac cttgacagca tccttagtca tgctttcagt
     6541 ccattatgca ataataggcc caggattgca ggcaaaagcc acaagagagg cccagaaaag
     6601 gacagctgct gggatcatga aaaatcccac agtggacggg ataacagtaa tagatctaga
     6661 accaatatcc tatgacccaa aatttgaaaa gcaattaggg caggtcatgc tactagtctt
     6721 gtgtgctgga caactactct tgatgagaac aacatgggct ttctgtgaag tcttgacttt
     6781 ggccacagga cctatcttga ccttgtggga gggcaacccg ggaaggtttt ggaacacgac
     6841 catagccgta tccaccgcca acattttcag gggaagttac ttggcgggag ctggactggc
     6901 tttttcactc ataaagaatg cacaaacccc taggagggga actgggacca caggagagac
     6961 actgggagag aagtggaaga gacagctaaa ctcattagac agaaaagagt ttgaagagta
     7021 taaaagaagt ggaatactag aagtggacag gactgaagcc aagtctgccc tgaaagatgg
     7081 gtctaaaatc aagcatgcag tatctagagg gtccagtaag atcagatgga ttgttgagag
     7141 agggatggta aagccaaaag ggaaagttgt agatcttggc tgtgggagag gaggatggtc
     7201 ctattacatg gcgacactca agaacgtgac tgaagtgaaa gggtatacaa aaggaggtcc
     7261 aggacatgaa gaaccgattc ccatggctac ttatggctgg aatttggtca aacttcattc
     7321 aggggttgac gtgttctaca aacccacaga gcaagtggac accctgctct gtgatattgg
     7381 ggagtcatct tctaatccaa caatagagga aggaagaaca ttaagagttt tgaagatggt
     7441 ggagccatgg ctctcttcaa aacctgaatt ctgcatcaaa gtccttaacc cctacatgcc
     7501 aacagtcata gaagagctgg agaaactgca gagaaaacat ggtgggaacc ttgtcagatg
     7561 cccgctgtcc aggaactcca cccatgagat gtattgggtg tcaggagcgt cgggaaacat
     7621 tgtgagctct gtgaacacaa catcaaagat gttgttgaac aggttcacaa caaggcatag
     7681 gaaacccact tatgagaagg acgtagatct tggggcagga acgagaagtg tctccactga
     7741 aacagaaaaa ccagacatga caatcattgg gagaaggctt cagcgattgc aagaagagca
     7801 caaagaaacc tggcattatg atcaggaaaa cccatacaga acctgggcgt atcatggaag
     7861 ctatgaagct ccttcgacag gctctgcatc ctccatggtg aacggggtag taaaactgct
     7921 aacaaaaccc tgggatgtga ttccaatggt gactcagtta gctatgacag atacaacccc
     7981 ttttgggcaa caaagagtgt tcaaagagaa ggtggatacc agaacaccac aaccaaaacc
     8041 cggtacacga atggttatga ccacgacagc caattggctg tgggccctcc ttggaaagaa
     8101 gaaaaatccc agactgtgca caagggaaga gttcatctca aaagttagat caaacgcagc
     8161 cataggcgca gtctttcagg aagaacaggg atggacatca gccagtgaag ctgtgaatga
     8221 cagccggttt tgggaactgg ttgacaaaga aagggcccta caccaggaag ggaaatgtga
     8281 atcgtgtgtc tataacatga tgggaaaacg tgagaaaaag ttaggagagt ttggcagagc
     8341 caagggaagc cgagcaatct ggtacatgtg gctgggagcg cggtttctgg aatttgaagc
     8401 cctgggtttt ttgaatgaag atcactggtt tggcagagaa aattcatgga gtggagtgga
     8461 aggggaaggt ctgcacagat tgggatatat cctggaggag atagacaaga aggatggaga
     8521 cctaatgtat gctgatgaca cagcaggctg ggacacaaga attactgagg atgaccttca
     8581 aaatgaggaa ctgatcacgg aacagatggc tccccaccac aagatcctag ccaaagccat
     8641 tttcaaacta acctatcaaa acaaagtggt gaaagtcctc agacccacac cgagaggagc
     8701 ggtgatggat atcatatcca ggaaagacca aagaggtagt ggacaagttg gaacatatgg
     8761 cttgaacaca ttcaccaaca tggaagttca actcatccgc caaatggaag ctgaaggagt
     8821 catcacacaa gatgacatgc agaacccaaa agggttgaaa gaaagagttg agaaatggct
     8881 gaaagagtgt ggtgtcgaca ggttaaagag gatggcaatc agtggaaacg attgcgtggt
     8941 gaagccccta gatgagaggt ttggcacttc cctcctcttc ttgaacgaca tgggaaaggt
     9001 gaggaaagac attccgcagt gggaaccatc taagggatgg aaaaactggc aagaggttcc
     9061 tttttgctcc caccactttc acaagatctt tatgaaggat ggccgctcac tagttgttcc
     9121 atgtagaaac caggatgaac tgatagggag agccagaatc tcgcaggggg ctggatggag
     9181 cttaagagaa acagcctgcc tgggcaaagc ttacgcccag atgtggtcgc ttatgtactt
     9241 ccacagaagg gatttgcgtt tagcctccat ggccatatgc tcagcagttc caacggaatg
     9301 gtttccaaca agcagaacaa catggtcaat ccacgctcat caccagtgga tgaccactga
     9361 agatatgctc aaagtgtgga acagagtgtg gatagaagac aaccctaata tgactgacaa
     9421 gactccagtc cattcgtggg aagatatacc ttacctaggg aaaagagagg atttgtggtg
     9481 tggatccctg attggacttt cttccagagc cacctgggcg aagaacattc acacggccat
     9541 aacccaggtc aggaacctga tcggaaaaga ggaatacgtg gattacatgc cagtaatgaa
     9601 aagatacagt gctccttcag agagtgaggg agttctgtaa tcaccaacaa caaacaccaa
     9661 aggctattga agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc
     9721 cgccaataat gggaggcgta ataatcccca gggaggccat gcgccacgga agctgtacgc
     9781 gtggcatatt ggactagcgg ttagaggaga cccctcccat cactgacaaa acgcagcaaa
     9841 agggggcccg aagccaggag gaagctgtac tcctggtgga aggactagag gttagaggag
     9901 acccccccaa cacaaaaaca gcatattgac gctgggaaag accagagatc ctgctgtctc
     9961 tgcaacatca atccaggcac agagcgccgc aagatggatt ggtgttgttg atccaacagg
    10021 ttctccgcgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    10081 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10141 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10201 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10261 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10321 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10381 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10561 aggactataa agataccagg cgtttccccc trraagctcc ctcgtgcgct ctcctgttcc
    10621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10921 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11461 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11521 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11581 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11641 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11701 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11761 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11821 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11881 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11941 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12001 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12061 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12181 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    12241 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
    12301 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
    12361 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
    12421 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
    12481 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
    12541 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
    12601 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
    12661 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
    12721 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
    12781 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
    12841 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
    12901 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
    12961 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
    13021 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
    13081 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
    13141 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
    13201 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
    13261 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
    13321 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
    13381 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
    13441 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
    13501 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
    13561 tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata
    13621 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    13681 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    13741 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    13801 cggcatcaga gcagattgta ctgagagtgc accatagatc aacgacatta ctatatatat
    13861 aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg
    13921 ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa
    13981 tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa
    14041 aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa
    14101 gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt
    14161 ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc
    14221 tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc
    14281 aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt
    14341 ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg
    14401 cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg
    14461 tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc
    14521 ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga
    14581 acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt
    14641 acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa
    14701 tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa
    14761 caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt
    14821 taagtattgt ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag
    14881 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
    14941 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
    15001 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
    15061 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
    15121 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    15181 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    15241 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
    15301 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt
    15361 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    15421 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    15481 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
    15541 gcgaattggg tacctattta ggtgacacta tag
//
