LOCUS       sequence-22729-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-SENP1 (catalytic domain)
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggtgccg cgcggcagcc atatggaatt tcctgaaatt acagaggaaa
      121 tggagaaaga aataaagaat gtatttcgta atgggaatca ggatgaagtt ctcagtgaag
      181 catttcgcct gaccattaca cgcaaagata ttcaaactct aaaccatctg aattggctca
      241 atgatgagat catcaatttc tacatgaata tgctgatgga gcgaagtaaa gagaagggct
      301 tgccaagtgt gcatgcattt aatacctttt tcttcactaa attaaaaacg gctggttatc
      361 aggcagtgaa acgttggaca aagaaagtag atgtattttc tgttgacatt cttttggtgc
      421 ccattcacct gggagtacac tggtgtctag ctgttgtgga ctttagaaag aagaatatta
      481 cctattacga ctccatgggt gggataaaca atgaagcctg cagaatactc ttgcaatacc
      541 taaagcaaga aagcattgac aagaaaagga aagagtttga caccaatggc tggcagcttt
      601 tcagcaagaa aagccaggag attcctcagc agatga
//
