LOCUS       sequence-22730-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-SENP2 (catalytic domain)
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggatctc cttgaactta cagaggacat ggaaaaggaa
      121 atcagtaatg ccctaggcca tggcccacag gatgaaatcc taagtagtgc tttcaaattg
      181 cgaattactc gaggagatat tcagacatta aagaactatc actggctcaa tgatgaagtc
      241 attaattttt acatgaatct tctggtggaa agaaataaaa agcaaggcta tccagcactt
      301 catgtattca gtactttctt ctatcctaaa ttaaagtctg ggggttacca agcagtgaaa
      361 cgatggacca aaggggtaaa tctctttgaa caagaaatta ttctggtgcc tattcatcgg
      421 aaggtacatt ggagcctggt ggtgattgac ctaagaaaaa agtgtcttaa atatctggat
      481 tctatgggac aaaagggcca caggatctgt gagattctcc ttcagtattt acaggatgaa
      541 agtaagacca aaagaaatag tgatctgaat cttttagagt ggacccatca cagcatgaaa
      601 ccacacgaga ttcctcaaca gctgaatggg agtgatt
//
