LOCUS       sequence-227302-        1266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pXPR_sgFLNB-SK2
FEATURES             Location/Qualifiers
     source          1..1266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        90..165
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    221..338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        496..707
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 cngggggggc ggggctcggc cggttcgatt tcttggcttt atatatcttg tggaaaggac
       61 gaaacaccgc cactgaccag gccacagatg ttttagagct agaaatagca agttaaaata
      121 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc
      181 gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      241 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      301 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      361 gagatccact ttggcgccgg ctcgaggggg cccgggtgca aagatggata aagttttaaa
      421 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
      481 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      541 gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      601 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      661 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
      721 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
      781 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      841 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      901 ggcgctgggg ccgccgcgtg cgaatctggt gcaccttcgc gcctgtctcg ctgctttcga
      961 taagtctcta gccatttaaa ttttgatgac ctgctgcgac gctttttttc tggcaggata
     1021 gtctgtaaat gcggccaaga tctgcacact ggtatttcgg ttttgggccg cgggcggcga
     1081 cggcccgngt ccagcgcaca tgttcgcgag gcgggctgca gccggccacg agatcggacg
     1141 ggtagtctca agctgcggct ggctgnctgg cctccgcccg cggtatcgcc cgctggcgca
     1201 agctgcgttg gnnntcgtga naatgcnntc gctgcaggac taatggagaa ccgcccctcn
     1261 aagcng
//
