LOCUS       sequence-227303-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pXPR_sgFLNB-SK4
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        69..144
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    200..317
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        475..686
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gaatttcgat ttctggcttt atatatcttg tggaaggacg aaacaccgtg acttccccat
       61 ctgtggccgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      121 gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg taactagatc ttgagacaaa
      181 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      241 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      301 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
      361 tcgagggggc ccgggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
      421 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      481 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
      541 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      601 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      661 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      721 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      781 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      841 gagccccttc gcctcgtgct tgggttgagg cctggcctgg gcgctggggc cgccgcgtgc
      901 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      961 attttgatga cctgctgcga cgcttttttt ctggcaagat agtctgtaaa tgcgggccaa
     1021 gatctgcanc tggtatttcg tttttgggcc gcggcggcga cggcccgtgc gttccagcca
     1081 aatgtcgcga ggcggcctgc aagccgacca ccgagaaatc ggacgggggt agtcncaagc
     1141 tgcgnctgtc tgtncatgac ctcgccgcnn ttatcgcccg cctgggccaa gctgccngta
     1201 cgacctcgta cnaagtggcc gtcgncgtgc tgcacggacc nnataggt
//
