LOCUS       sequence-227305-        1272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pXPR_sgGFP3
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        84..159
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    215..332
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        490..701
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gnctgtgggg gccgggcgat tcgattcttg gctttatata tcttgtggaa aggacgaaac
       61 accgagctgg acggcgacgt aaagttttag agctagaaat agcaagttaa aataaggcta
      121 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct tggcgtaact
      181 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      241 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      301 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      361 cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt taaacagaga
      421 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      481 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      541 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      601 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      661 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      721 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      781 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      841 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctggcgctg
      901 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
      961 tctagccatt taaattttga tgacctgctg cgacgctttt tttctggcaa gatagtctgt
     1021 aaatgcggcc agatctgcac actggtattt cgtttggggc cgcggcggcg acgggcccgg
     1081 tgcgtccagg cacatgttcg gcgggcgggc ctgcagccgg cacggagatc gacgggtagt
     1141 ctcaagctgg cggctgctct ggctggcctc cgccgcgnnt ccccgccttg gcgcagctgc
     1201 ngtgncnnnc gtgagcnaag tggccntcgn ctgtcgagga gacacaattg ggnnncgngc
     1261 tctctnggaa gc
//
