LOCUS       sequence-227306-        1267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pXPR_sgGFP4
FEATURES             Location/Qualifiers
     source          1..1267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        70..145
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    201..318
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        476..687
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gangatgcga tttcttggct ttatatatct tgtggaagga cgaaacaccg gccacaagtt
       61 cagcgtgtcg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      121 tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat cttgagacaa
      181 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
      241 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
      301 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
      361 ctcgaggggg cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc
      421 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
      481 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt
      541 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      601 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      661 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      721 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      781 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
      841 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg gcgctggggc cgccgcgtgc
      901 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga tagtctctag ccatttaaaa
      961 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcggccag
     1021 atctgcacac ctggtattcc ggttttgggg ccgcggcggc gaacggccgt gcgtccagcg
     1081 ccacattgtt cggcgagcgg ggcctgcagc gcggccacgg tatcggacgg ggttagtctc
     1141 agctggccgc tgctngggtg ctggcctccc acacnnntat cgccncngag ccaagcctgc
     1201 ccgtcgnnga tgcgacgaaa atgnnntcgc catgtgctgc gggacatctc natgtgaatg
     1261 caacagc
//
