LOCUS       sequence-227308-       12247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette 
            into the mouse TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 12247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06"; journalName: "Cell"; date: "2018-07-12- 
            12"; volume: "174"; issue: "2"; pages: "465-480.e22"
COMMENT     Sequence Label: Ai133 (TITL-ssAPEX2tm) targeting vector
FEATURES             Location/Qualifiers
     source          1..12247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        3009..3323
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3017..3035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3088..3106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3124..3142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3160..3178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3195..3213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3231..3249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3296..3320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3333..3366)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3443..3491
                     /note="synthetic polyadenylation signal"
     polyA_signal    3500..3976
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3699..3718)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     polyA_signal    3988..4449
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4476..4509)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4592..4615
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5369..5395
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    5495..6083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5548..5568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5966..5977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6108..6125)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6114..6321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8819..8852
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        8879..9378
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8914..8937)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9311..9330
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10026..10042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10026..10042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10039..10061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10050..10066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10074..10080,10081..10098,10099..10104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10119..10140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10257..10274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10428..11016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10508..10527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11187..11978,11979..12047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11810..11829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12048..12152)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12220..12238
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagcg
     1741 agctcggtac ccagagcccc atcctcactg actccgtcct ggagttggat gagagataat
     1801 ggccttacgt tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa
     1861 gagcggtgct gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt
     1921 gatgaagaac tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt
     1981 tggggttggt cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat
     2041 gaacatctac atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct
     2101 ctccaggcaa gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct
     2161 cagacacagg gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta
     2221 acttgctctt tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga
     2281 cttggagagt gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac
     2341 ttccttatcc ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc
     2401 cagaccaaca atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac
     2461 tgagacaggg gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga
     2521 cgcactgaac aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc
     2581 caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg
     2641 gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc
     2701 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
     2761 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     2821 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     2881 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     2941 tttggggggg ggctgtccct ctaggggtac ccggggatcc accggtatcg attacctatc
     3001 acaaattcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     3061 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     3121 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3181 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     3241 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
     3301 gtttagtgaa ccgtcagatc gcctatttaa atataacttc gtatagcata cattatacga
     3361 agttattcgc gatgaataaa tgaaagcttg cagatcccct cagcaccatg gctagcggca
     3421 gcctcggagt ttgaatagat agaataaaat atctttattt tcattccatc tgtgtgttgg
     3481 ttttttgtgt gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     3541 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     3601 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     3661 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3721 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3781 gcctcaggct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     3841 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     3901 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3961 tcccttccct gtccttctgc ctcagcgctg taagtctgca gaaattgatg atctattaaa
     4021 caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag
     4081 aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg
     4141 attagataaa tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc
     4201 atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc
     4261 actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca
     4321 ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag
     4381 atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt
     4441 ccatcagaaa gcttttaatt aactgaatag ctaacataac ttcgtatagc atacattata
     4501 cgaagttatc acgcgtgcca ccatgaaatg taaattcctg ggtagcttct ttctgctctt
     4561 cagcctttcc ggcaaagggg cggactgcat ggactataaa gacgacgacg ataaaggaaa
     4621 gagctaccca acagtgtccg ctgactatca ggatgctgtc gaaaaagcaa agaaaaagct
     4681 gagggggttt atcgctgaga agagatgcgc accactgatg ctccggctgg ccttccactc
     4741 cgctggtacc tttgacaaag gcaccaagac aggcggaccc ttcggaacca tcaagcaccc
     4801 tgcagaactc gcccattctg ctaacaatgg cctggacatc gccgtgagac tgctcgaacc
     4861 tctcaaggcc gagttcccaa ttctgagtta cgctgacttt tatcagctgg caggcgtggt
     4921 cgccgtggag gtcactgggg gtcctaaagt gccattccac ccaggacgag aggacaagcc
     4981 tgaaccacct ccagagggac gactgccaga tcccaccaaa gggagcgacc atctgaggga
     5041 cgtgttcggc aaggcaatgg gcctcactga ccaggacatc gtggccctgt ccggcggaca
     5101 caccattgga gccgctcata aggaaagaag tggattcgag gggccctgga ccagcaaccc
     5161 cctgatcttc gacaattcct actttactga gctgctctct ggagagaaag aagggctgct
     5221 ccagctgcca tcagacaagg ctctgctcag cgaccccgtg ttcaggccac tggtcgacaa
     5281 atacgcagcc gacgaagatg cattctttgc cgattatgct gaagcacatc agaagctctc
     5341 tgagctggga ttcgcagacg ctctccagct gccacctctg gagcgcctca ccctggatgt
     5401 cacagtgggg gtcggtgcag gaggactcct cttggtgctc ttggtggcgc tttttatttt
     5461 ctgtatctga acgcgttacg taaagcttgt cgataatcaa cctctggatt acaaaatttg
     5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6061 ctccctttgg gccgcctccc cgcatcgaca ccgagcgctg ctcgaggcct cgactgtgcc
     6121 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6181 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6241 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6301 caatagcagg catgctgggg acacgtggga tcctctagag ccccatcctc actgactccg
     6361 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     6421 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     6481 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     6541 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     6601 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     6661 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     6721 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     6781 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     6841 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     6901 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     6961 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     7021 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     7081 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     7141 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     7201 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     7261 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     7321 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     7381 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     7441 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     7501 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc
     7561 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     7621 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     7681 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     7741 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     7801 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     7861 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     7921 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     7981 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     8041 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     8101 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     8161 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     8221 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     8281 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     8341 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     8401 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     8461 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     8521 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     8581 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     8641 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     8701 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     8761 tcgacggcgc gccgtaaaag cttggcatag atcttattgc agaagccata tgttttaggt
     8821 gccagggcgt gcccttgggc tccccgggcg cgtttgggcg gccgcatcga attctaccgg
     8881 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     8941 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     9001 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     9061 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     9121 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     9181 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     9241 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     9301 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     9361 tcatctccgg gcctttcgac ctgcaatcgc cgctagccgc caccatgaaa aagcctgaac
     9421 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     9481 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     9541 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     9601 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     9661 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     9721 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     9781 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     9841 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     9901 tggacgacac cgtcagtgta agtgctagcc cctaggcctg aatcgaagtt cctattcttc
     9961 aaaaggtata ggaacttcga gctcgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
    10021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10141 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10201 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    12181 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    12241 tttcgtc
//
