LOCUS       sequence-22731-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-SENP5 (catalytic domain)
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             678..695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attcccncta naatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgctg gatatggacg
      121 acctggcgac tctggatggt cagaactggc tgaatgacca ggtcattaat atgtatggtg
      181 agctgataat ggatgcagtc ccagacaaag ttcacttctt caacagcttt tttcatagac
      241 agctggtaac caaaggatat aatggagtaa aaagatggac taaaaaggtg gatttgttta
      301 aaaagagtct tctgttgatt cctattcacc tggaagtcca ctggtctctc attactgtga
      361 cactctctaa tcgaattatt tcattttatg attcccaagg cattcatttt aagttttgtg
      421 tagagaatat aagaaagtat ttgctgactg aagccagaga aaaaaataga cctgaatttc
      481 ttcagggttg gcagactgct gttacgaagt gtattccaca acagaaaaac gacagtgact
      541 gtggagtctt tgtgctccag tactgcaagt gcctcgcctt agagcagcct ttccagtttt
      601 cacaagaaga catgccccga gtgcggaaga ggatttacaa ggagctatgt gagtgccggc
      661 tcatggactg actcgagcac caccaccacc accactgaga tccggctgct aacaancccg
      721 aaggaactga ttggctgngc ccccctgaca aaccagcaan ccctgggcct ctancggtct
      781 ganggtttng ngaaggagac tat
//
