LOCUS       sequence-227313-        8801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Piggybac transposon plasmid CRISPR gene deletion activatable 
            fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter
            with two SV40 polyA followed by red-enhanced nanolantern (ReNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Rui Y, Wilson DR, Choi J, Varanasi M, Sanders K, Karlsson J, Lim M, 
            Green JJ
  TITLE     Carboxylated branched poly(beta-amino ester) nanoparticles enable 
            robust cytosolic protein delivery and CRISPR-Cas9 gene editing.
  JOURNAL   Sci Adv. 2019 Dec 6;5(12):eaay3255. doi: 10.1126/sciadv.aay3255. 
            eCollection 2019 Dec.
  PUBMED    31840076
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-12-6"; volume: "5(12):eaay3255. doi"; pages: " 
            10.1126/sciadv.aay3255. eCollection 2019 Dec"
COMMENT     Sequence Label: PB-iRFP-STOP-ReNL
FEATURES             Location/Qualifiers
     source          1..8801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    59..193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(199..394)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      213..348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    453..474
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(489..494,495..512,513..519)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    527..543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     532..554
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     551..567
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     551..567
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   590..624
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1277..1296
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1295..1350
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1396..1415
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    complement(3427..3460)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3489..3623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3743..3877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4044..4077)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4099..4302)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4128..4148)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4303..4606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    4770..4881
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4907..5842)
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     regulatory      5839..5848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5885..5905)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             5968..6236
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(6249..6460)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    complement(6539..6572)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6869..6931
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        6969..7073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7074..7142,7143..7934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7292..7311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8105..8693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8594..8613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcgga
       61 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      121 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      181 caataaacaa gttagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
      241 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
      301 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
      361 gggcgggact atggttgctg acttattgag atgcggatcc gctggcacga caggtttccc
      421 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      481 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      541 caatttcaca caggaaacag ctatgaccat gattacgcca agcttttaac cctagaaaga
      601 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
      661 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
      721 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
      781 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
      841 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcgt
      901 gactaatata taataaaagc attacacgtc ttgagcgatt gtgtaggctg gagctctgca
      961 ggtcgaggga tctccataag agaagaggga cagctatgac tgggagtagt caggagagga
     1021 ggaaaaatct ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata
     1081 acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg
     1141 aatggacagc agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca
     1201 tggcagccag catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt
     1261 ttaaatgatt tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca
     1321 ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca
     1381 tgatgtcccc ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg
     1441 cattggccac accagccacc accttctgat aggcagcctg cacctgagga gtgcggccgc
     1501 ttacgccaga atgcgttcgc acagccgcca gccggtcact ccgttgatgg ttactcggaa
     1561 cagcagggag ccgtcggggt tgatcaggcg ctcgtcgata attttgttgc cgttccacag
     1621 ggtccctgtt acagtgatct ttttgccgtc gaacacggcg atgccttcat acggccgtcc
     1681 gaaatagtcg atcatgttcg gcgtaacccc gtcgattacc agtgtgccat agtgcaggat
     1741 caccttaaag tgatgatcat ccacagggta caccacctta aaaatttttt cgatctggcc
     1801 catttggtcg ccgctcagac cttcatacgg gatgatgaca tggatgtcga tcttcagccc
     1861 attttcaccg ctcaggacaa tcctttggat cggagttacg gacaccccga gattctgaaa
     1921 caaactggac acacctccct gttcaaggac ttggtccagg ttgtagccgg ctgtctgtcg
     1981 ccagtcccca acgaaatctt ccagccggaa caggtggtgg cggccctcgg agcgctcgta
     2041 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta
     2101 gtagtagccg ggcagttgca cgggcttctt ggccatgtag atggtcttga actccaccag
     2161 gtagtggccg ccgtccttca gcttcagggc ctggtggatc tcgcccttca gcacgccgtc
     2221 gcgggggtac aggcgctcgg tggaggcctc ccagcccatg gtcttcttct gcattacggg
     2281 gccgtcgggg gggaagttgg tgccgcgcat cttcaccttg tagatcagcg tgccgtcctg
     2341 cagggaggag tcctgggtca cggtcaccag accgccgtcc tcgaagttca tcacgcgctc
     2401 ccacttgaag ccctcgggga aggacagctt cttgtaatcg gggatgtcgg cggggtgctt
     2461 cacgtacgcc ttggagccgt acatgaactg gggggacagg atgtcccagg cgaagggcag
     2521 ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
     2581 gccctcgccc tcgatctcga actcgtggcc gttcatggag ccctccatgc gcaccttgaa
     2641 gcgcatgaac tctttgatga cggccatgtt gttgtcctcg gaggaggcgg tgccggagct
     2701 gccgctgccg gtgctgccgg tgccatgccc caggaacagg tggtggcggc cctcggagcg
     2761 ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc
     2821 cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg tcttgaactc
     2881 caccaggtag tggccgccgt ccttcagctt cagggcctgg tggatctcgc ccttcagcac
     2941 gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct tcttctgcat
     3001 tacggggccg tcggggggga agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc
     3061 gtcctgcagg gaggagtcct gggtcacggt caccagaccg ccgtcctcga agttcatcac
     3121 gcgctcccac ttgaagccct cggggaagga cagcttcttg taatcgggga tgtcggcggg
     3181 gtgcttcacg tacgccttgg agccgtacat gaactggggg gacaggatgt cccaggcgaa
     3241 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     3301 gccctcgccc tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgcgcac
     3361 cttgaagcgc atgaactctt tgatgacctc ctcgcccttg ctcaccatga attcgctagc
     3421 tctagaataa cttcgtatag catacattat acgaggttat attaagggtt ccggatcagc
     3481 ttgatgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3541 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3601 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3661 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     3721 attatgatcc tctagagtcg cagatccaga catgataaga tacattgatg agtttggaca
     3781 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     3841 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3901 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     3961 atgtggtatg gctgattatg atcctctaga gtcgcagatc ctctagagtc gcagatctgc
     4021 aagctttcat ttattcatcg cgaataactt cgtatagcat acattatacg aggttatgat
     4081 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     4141 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     4201 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     4261 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     4321 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     4381 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     4441 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     4501 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     4561 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     4621 tgggctatga actaatgacc ccgtaattga ttactatagg gatgttttca cttgcggccg
     4681 gaggggaagg agtgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     4741 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     4801 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     4861 gctggcaact agaaggcaca gtcggtacgc gttaagtgca acacgcttag cgttggtggt
     4921 gggcggcggt gaagtgcagc gataagaagt cggcgaacat ttcggccgcg acgcgctgcg
     4981 ccatcggcac ggcacgcggc tcgtaatgat gacagatgat caatccccat agcgtgccgt
     5041 caatgatgat cgacagcgac atcgaggcgc tgacgcccat gttccgcaga aattcgagat
     5101 ggcagggcga gatgctgcgc aggtgcgcga acgacagatc gagcgcggcg ccggaggcgt
     5161 cgtgctcggg cacgatccgg ctgctgatgc cgcgcgaatc cgagaccacg cggatcgcgt
     5221 tcttcaagta cagtagccgc gcctgctgcg ggaccagcga cgccggaaag tgctgaccga
     5281 gaaagctctc gaggtcgctg cgcttcgcct cgccgatcac catcccggag ccgtcgtccg
     5341 cgaagcggta caacatcacg cggtgatagc cgagcatcgc ctgcagatag cgcggcaccc
     5401 gtgcggccat ctcttcgagc gacttcagtt ctttggtgcg cgcgatgatc tgccgcgtca
     5461 gccgcagcgg attgtcggcc tgttcggccg ccgcaggctc gaactcgatg atcgatgtac
     5521 cgtcatggcg atgcagtgag atgtcgaagg tgcggccggt caggccgtcg cgccaaccga
     5581 agatcagcgc cggtcgcttt ggatcggagg actgcgccag tgcgttgcgc agcgcatggg
     5641 cttcggtctc gccgaagtaa tcggcgagta gctcaccgac ccgcggagtt tcgcgtccaa
     5701 agaacgcgcc ggcattttcc gtaatgcgcg tgatccgcac cgcctgcgcg tcgcaggcta
     5761 gcaggcagcc gcacggctga atgctgccgg ggatgtggat cggctcgcga tcgcaggagg
     5821 tgagatcgac cttacgcgcc atggtggcga ccggggtggc cactttgtag aaagctgggt
     5881 accgtacctt acgcttcttc tttggagcca tagggatgtt ttcacttgcg gccggagggg
     5941 aaggaggtag gcgccggtca cagcttggat ctgtaacggc gcagaacaga aaacgaaaca
     6001 aagacgtaga gttgagcaag cagggtcagg caaagcgtgg agagccggct gagtctaggt
     6061 aggctccaag ggagcgccgg acaaaggccc ggtctcgacc tgagctttaa acttacctag
     6121 acggcggacg cagttcagga ggcaccacag gcgggaggcg gcagaacgcg actcaaccgg
     6181 cgtggatggc ggcctcaggt agggcggcgg gcgcgtgaag gagagatgcg agcccctcga
     6241 agcttcagct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact
     6301 gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca
     6361 ctttcccagt ttaccccgcg ccaccttctc taggcacccg ttcaattgcc gacccctccc
     6421 cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcga
     6481 tcgcagatcc ttgcacgaga ctagttggac tactgcgccc tacagatccc ttaatcgcat
     6541 aacttcgtat agcatacatt atacgaagtt atgcgattaa taagtataat ttgtttctat
     6601 tatgtataag ttaagctaat tacttatttt ataatacaac atgactgttt ttaaagtaca
     6661 aaataagttt atttttgtaa aagagagaat gtttaaaagt tttgttactt tatagaagaa
     6721 attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat
     6781 ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat
     6841 aaacctcgat atacagatcg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa
     6901 cgtacgtcac aatatgatta tctttctagg gttaagtcga cccaggtggc acttttcggg
     6961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8761 ctgcgttatc ccctgattct gtggataacc gtattaccgc c
//
