LOCUS       sequence-227319-       12452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW646 - pML1>>
FEATURES             Location/Qualifiers
     source          1..12452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             5943..6284
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      6341..6982
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     protein_bind    7088..7107
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7140..7159
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7192..7211
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7244..7263
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        7287..7333
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(7418..7420,7421..7423,7424..8134)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7463..7484)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7724..7743)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8071..8092
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8798..9178
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    10471..10495
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(10586..11174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10666..10685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11345..12133)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(12424..12452,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta
      601 attaaacacg gcgcgccaac agcgatcgca ccaggtacca cctagcttat caaagaaaaa
      661 acaagaacaa aacgatgcat agtttctaaa atgtgctaaa attcagaaac tgaaacatga
      721 ttcattgtct gaaactttgt ttcaaattac tgaaaataat cattcactgg accaaaacaa
      781 ataaataaaa taaaatcgaa tttctgaatt tggaaattgg tttttggttt ttaattttaa
      841 acaaaacaaa aacgaaattt gaaggcaata aatgagttag ttggtaggca gaagtcactc
      901 gttcccacta gctattatta ttagaagaaa cgtccccaca actccaaggc gtttcagttc
      961 ctttaattta ctgaattacc ctcctcatat ctataaaaaa tcacctcttg taccaatgcc
     1021 ccatttacac atcctgtcgt ttatttctag actaagtgga ctacatgtcg gttatttgat
     1081 tcgcaccatg cgtatttgga ttatcgctaa cacacccctt caaacaatac gcttaactcg
     1141 tattacaaaa tttcaagtga tgaattatct atgtataaga tatagatagg aacaactaag
     1201 catcgagaaa tttgtatata aatcaactag acttatatat atttcgatac agaatttata
     1261 cgtattatat caaattaatt agtaattgtt tcctctacgt gagtttaatt aacaatgata
     1321 agctacattg agtgtatcag ttctaaaact ttatagtatg ctacaatcaa tttttctaag
     1381 taacaacttc aagcaaggaa tcacacacac acagtggtac ataataaact tgattttaat
     1441 atcatatgat cagcatcatt aacggaataa gttaagtaat tcgtcatcca tactactaag
     1501 tcatattaaa atcataatca aacttaaaag ccgattagaa agagagcaaa tatatctaaa
     1561 aattcacgag gaagacgaca aatgcaagga aacacagcta gtattattaa acttaataga
     1621 tattggatga atgactgcat aatatatatc acattaaaag tggacataaa tttgcatatg
     1681 tgtaatgtac ctctccacaa ttaatcgcgg accatttatt ttactattac aagtcaagta
     1741 actttatatt gttgatccat aattcttttc gaacataaaa tcatatactt aggccatttt
     1801 caactgtcaa aactcgaatc cgagaaccaa atttcaccat tttccaaaaa tgatgagtgt
     1861 cgaccaaatg gggtactact gtctaatcag gaacttgtga acaaattttc aaccttttcc
     1921 aaataagacg agtgtcaacc aactttttcc aaccaagaga tattgggttg ctacacaaat
     1981 acttaatagc cattgcatat ttatgcatat gcaaatgcag ggtcgtggcg tcagaaagaa
     2041 acataggacc ctcaacatat ttaatatttt gggagctata tttgactatt tcatattaga
     2101 aaataataat aaaaaagtgt tggttttata tcaaattgta atttacgaaa aacttatgct
     2161 tttgcgcaat gatttttgta aagtatctac tatgtttagt gtttacattg attagtaggc
     2221 tgccgttttt tttcttgtgt attatgtact atatatgaat atgaacattt gtaaaagtga
     2281 atcttgtcat tttcttgttg aaaacatata tagtatgtgc aaacaaagca taggttaatc
     2341 caataccaca caaataacac gtcaggtaaa tccaataata aatcgtatgt gcatgtatgt
     2401 gtattcatgt atgttacatg aatgtctgaa tcagtcagtg tacgtatatg atgtaggtga
     2461 tgtaaatctt aatgtatgag ctgtttcttg gaccatggtc cacaatggat attgctcccc
     2521 aactacatta gtcaatcgac tggccaattt ttaattaaga taattaatcc aaactaccat
     2581 taaatataac tttgaccttt tttctattca tttttagata ttattggaac ttacgtagtt
     2641 tacatgcatc tcatcccttt cttttgctcc ttgaaagtgg gtccaatcac aaaaaatgat
     2701 cttatatttt gtattttgta ttttaaaaac tcataattat ataggttcaa aaatttaatt
     2761 aacatcagtg tatactataa ttactactct agccaacaag ataaattcat tttgacatca
     2821 gccaaaagat aaaaatttgg ttaaaaacta ttggattagc ttttagtatt taatatttta
     2881 tgtactgatt aaatacgaat ttagaaatct aggatataag tgagggtgta taataaggga
     2941 ggggtggacc attaatagcg atgtgcaatt aaaaattatg attaagaatc taggaaattt
     3001 gtagattgct tagttatttt tatggcgatc gtcgtgtcaa tgtcatggat tttgaaactt
     3061 taaattaatc tcttaaatta gcacctacct ttgaatttta tagaatcttt ttattttata
     3121 tgtttaattt tatagaatct aactagctta ttttgagatt aaattgttta gttactttta
     3181 taacagtata aatgtataat gaggacctaa gaatgtagtc ctgtaatgtt cttgctattc
     3241 tacttaatct catcaccaat caaccatcaa aagaagctag tactaataaa acctgcaggt
     3301 attcgaataa taattaagct caaacactat actaatttat ggaggattat atattcaatg
     3361 aattaggaac ctcatgatgg acattattga ctgatataat gtgtatacta attgtgagta
     3421 tttaaaaacc atacaaagca tttatatgtc cacatatatt ggacacacat gcaatcaatg
     3481 ttcaatatgc tccacacaca gaaataaaaa tactctttct gatcatatga tacatcatac
     3541 atatactaaa aaaatctaaa atgaactata accacaagca tatataataa caatgaaatg
     3601 gtaatgtttc ttcattttta tttgttcaaa ttcttattcg gttgtttttt cttaccctac
     3661 gagaatccgt gaggtcaaag ggaaacagtg attttttttt tgtattttgt tttttaaatt
     3721 gatgaactgt aaaactctct ctctagaaaa atatataagt agtagtatga attttctctc
     3781 actaaaagca ttaatggacc tttcgataat cataaatgca atgcaccctc tctatgcatt
     3841 tcgcaataac tccttttcct tctgccacat cctcttcctc acctctttct cttcttccct
     3901 ttctcctaag ttcctcctcc accaaattct ccatttattt cgttaactat cctccatttg
     3961 ttttcttctg aagagtgata tattctacct ttctctggtt aaagaaactc cctgaatcca
     4021 ccggttaaac agtattcagt cgactggtac caacaggctc catggctagt gaagctcgaa
     4081 aaacaaagaa aaaaatcaaa gggattcagc aagccactgc aggagtctca caagacactt
     4141 cggaaaatcc taacaaaaca atagttcctg cagcattacc acagctcacc cctaccttgg
     4201 tgtcactgct ggaggtgatt gaacccgagg tgttgtatgc aggatatgat agctctgttc
     4261 cagattcagc atggagaatt atgaccacac tcaacatgtt aggtgggcgt caagtgattg
     4321 cagcagtgaa atgggcaaag gcgataccag gcttcagaaa cttacacctg gatgaccaaa
     4381 tgaccctgct acagtactca tggatgtttc tcatggcatt tgccctgggt tggagatcat
     4441 acagacaatc aagtggaaac ctgctctgct ttgctcctga tctgattatt aatgagcaga
     4501 gaatgtctct accctgcatg tatgaccaat gtaaacacat gctgtttgtc tcctctgaat
     4561 tacaaagatt gcaggtatcc tatgaagagt atctctgtat gaaaacctta ctgcttctct
     4621 cctcagttcc taaggaaggt ctgaagagcc aagagttatt tgatgagatt cgaatgactt
     4681 atatcaaaga gctaggaaaa gccatcgtca aaagggaagg gaactccagt cagaactggc
     4741 aacggtttta ccaactgaca aagcttctgg actccatgca tgaggtggtt gagaatctcc
     4801 ttacctactg cttccagaca tttttggata agaccatgag tattgaattc ccagagatgt
     4861 tagctgaaat catcactaat cagataccaa aatattcaaa tggaaatatc aaaaagcttc
     4921 tgtttcatca aaaatctact agcaaaccgg taacgttata cgacgtcgct gaatacgccg
     4981 gcgtttctca tcaaaccgtt tctagagtgg ttaaccaggc ttcacatgtt agcgctaaaa
     5041 cccgggaaaa agttgaagct gccatggctg agctcaacta catcccgaac cgtgttgcgc
     5101 agcagctggc tggtaaacaa agcttgctga tcggtgtcgc gacctcgagc ttggccctgc
     5161 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     5221 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     5281 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     5341 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     5401 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     5461 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     5521 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     5581 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     5641 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     5701 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     5761 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     5821 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     5881 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccactagt ggatcggaat
     5941 tcgccaattt taatcaaagt gggaatattg ctgatagctc attgtccttc actttcacta
     6001 acagtagcaa cggtccgaac ctcataacaa ctcaaacaaa ttctcaagcg ctttcacaac
     6061 caattgcctc ctctaacgtt catgataact tcatgaataa tgaaatcacg gctagtaaaa
     6121 ttgatgatgg taataattca aaaccactgt cacctggttg gacggaccaa actgcgtata
     6181 acgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
     6241 atctattcga tgatgaagat accccaccaa acccaaaaaa agagtcaggt ggatcctaga
     6301 taacctttac cttcattccc tgtaaattgg taccctgcaa agctttcgtt cgtatcatcg
     6361 gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct
     6421 actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt
     6481 gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg
     6541 agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt
     6601 ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt
     6661 gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca
     6721 cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa
     6781 agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt
     6841 cctttatgta attttccaga atccttgtca gattctaatc attgctttat aattatagtt
     6901 atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca
     6961 attgattgac aacatgcatc aatcgactat gagtaggcaa gatgttctgt aggggctatg
     7021 atgcctggtc acaacctatg catatgtcga gctcaagaaa tctagaaaga agaaagggaa
     7081 gagaaagaat tgtgagcgct cacaattgaa agactagaaa gaagaaaggg aagagaaaga
     7141 attgtgagcg ctcacaattg aaagactaga aagaagaaag ggaagagaaa gaattgtgag
     7201 cgctcacaat tgaaagacta gaaagaagaa agggaagaga aagaattgtg agcgctcaca
     7261 attgaaagac tagtggatcg atcttcgcaa gacccttcct ctatataagg aagttcattt
     7321 catttggaga ggacgaacaa tgaaagcctt cacactcgct ctcttcttag ctctttccct
     7381 ctatctcctg cccaatccag ccactagtgg ctcaacaatg gtgagcaagg gcgaggagct
     7441 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     7501 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     7561 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg
     7621 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     7681 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     7741 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     7801 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actactttag
     7861 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     7921 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     7981 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     8041 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     8101 cgggatcact ctcggcatgg acgagctgta caagtcaggt catgatgagc tttagctgca
     8161 tctcgtctct gttatgctta agaagttcaa tgtttcgttt catgtaaaac tttggtggtt
     8221 tgtgttttgg ggccttgtat aatccctgat gaataagtgt tctactatgt ttccgttcct
     8281 gttatctctt tctttctaat gacaagtcga acttcttctt tatcatcgct tcgtttttat
     8341 tatctgtgct tcttttgttt aatacgcctg caaagtgact cgactctgtt tagtgcagtt
     8401 ctgcgaaact tgtaaatagt ccaattgttg gcctctagta atagatgtag cgaaagtgtt
     8461 gagctgttgg gttctaagga tggcttgaac atgttaatct tttaggttct gagtatgatg
     8521 aacattcgtt gttgctaaga aatgcctgta atgtcccaca aatgtagaaa atggttcgta
     8581 cctttgtcca agcattgata tgtctgatga gaggaaactg caagatactg agcttggttt
     8641 aacgaaggag aggcagtttc ttccttccaa agcatttcat ttgacaatgc cttgatcatc
     8701 ttaagtagag tttctgttgt ggaaagtttg aaactttgaa gaaacgactc tcaagtaaat
     8761 tgatgatcac aagtgaaagt gtatgttact aggatccttt tcaaatcagt gcgcaagacg
     8821 tgacgtaagt atccgagtca gtttttattt ttctactaat ttggtcgttt atttcggcgt
     8881 gtaggacatg gcaaccgggc ctgaatttcg cgggtattct gtttctattc caactttttc
     8941 ttgatccgca gccattaacg acttttgaat agatacgctg acacgccaag cctcgctagt
     9001 caaaagtgta ccaaacaacg ctttacagca agaacggaat gcgcgtgacg ctcgcggtga
     9061 cgccatttcg ccttttcaga aatggataaa tagccttgct tcctattata tcttcccaaa
     9121 ttaccaatac attacactag catctgaatt tcataaccaa tctcgataca ccaaatcgcc
     9181 atggcttcta tgatatcctc ttcagctgtg actacagtca gccgtgcttc tacggtgcaa
     9241 tcggccgcgg tggctccatt cggcggcctc aaatccatga ctggattccc agttaagaag
     9301 gtcaacactg acattacttc cattacaagc aatggtggaa gagtaaagtg catggtgacg
     9361 gtgttcggca ttctgaatct caccgaggac tccttcttcg atgagagccg gcggctagac
     9421 cccgccggcg ctgtcaccgc ggcgatcgaa atgctgcgag tcggatcaga cgtcgtggat
     9481 gtcggaccgg ccgccagcca tccggacgcg aggcctgtat cgccggccga tgagatcaga
     9541 cgtattgcgc cgctcttaga cgccctgtcc gatcagatgc accgtgtttc aatcgacagc
     9601 ttccaaccgg aaacccagcg ctatgcgctc aagcgcggcg tgggctacct gaacgatatc
     9661 caaggatttc ctgaccctgc gctctatccc gatattgctg aggcggactg caggctggtg
     9721 gttatgcact cagcgcagcg ggatggcatc gccacccgca ccggtcacct tcgacccgaa
     9781 gacgcgctcg acgagattgt gcggttcttc gaggcgcggg tttccgcctt gcgacggagc
     9841 ggggtcgctg ccgaccggct catcctcgat ccggggatgg gatttttctt gagccccgca
     9901 ccggaaacat cgctgcacgt gctgtcgaac cttcaaaagc tgaagtcggc gttggggctt
     9961 ccgctacttg tctcggtgtc gcggaaatcc ttcttgggcg ccaccgttgg ccttcctgta
    10021 aaggatctgg gtccagcgag ccttgcggcg gaacttcacg cgatcggcaa tggcgctgac
    10081 tacgtccgca cccacgcgcc tggagatctg cgaagcgcaa tcaccatctc ggaaaccctc
    10141 gcgaaatttc gcagtcgcga cgccagagac agagggttag atcatgccta gcttggctgc
    10201 aggtcgacct gcaggcatgc ccgctgaaat caccagtctc tctctacaaa tctatctctc
    10261 tctataataa tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc
    10321 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
    10381 caataaaatt tctaattcct aaaaccaaaa tccaggggta ccgtattcta gaccaagcat
    10441 gcacagctgg cgttctcagc cagatcccct tgacaggata tattggcggg taaactaagt
    10501 cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc
    10561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    11041 tctgcgctct gctgaagcca gttaccttcg gaagaagagt tggtagctct tgatccggca
    11101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    11221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    11281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa cattggtcta
    11341 gtgattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca
    11401 actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa
    11461 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
    11521 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
    11581 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
    11641 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
    11701 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
    11761 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
    11821 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
    11881 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
    11941 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
    12001 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
    12061 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
    12121 ccgcttccct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
    12181 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
    12241 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
    12301 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
    12361 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga
    12421 tccccatcca acagcccgcc gtcgagcggg ct
//
