LOCUS       sequence-22733-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-SENP7 (catalytic domain)
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgacagat gcggccaagc
      121 ctacttacac cttcctgcag aagcaaagta gcggttgcta ctccctttct attacatcta
      181 atccagatga agaatggcga gaagtcaggc acactggact tgttcagaag ttgattgtat
      241 atcctccacc acctactaag gggggattag gagtaactaa tgaagatctg gagtgtttag
      301 aagaaggaga gtttcttaat gatgtaatca ttgattttta ccttaagtat cttatattgg
      361 agaaggcatc agatgaactt gttgaacgaa gtcacatttt tagtagcttt ttctataaat
      421 gcttgacaag aaaggaaaat aatttaacag aagataatcc aaatctttca atggcacaga
      481 gaagacataa aagagtaaga acatggactc gtcacataaa catttttaat aaagattaca
      541 tctttgtacc tgtaaatgag tcgtctcact ggtatctcgc agtcatttgt tttccatggt
      601 tagaagaagc tgtgtatgaa gattttccac aaactgtatc ccagcagtcc caggctcagc
      661 agtcccaaaa tgacaacaaa acaatagata atgatctacg tactacttcg acactgtctt
      721 tgagtgcaga ggattcccaa agtaccgagt cgaatatgtc agta
//
