LOCUS       sequence-22734-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-SENP8 (full length))
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             738..755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatncat catcatcatc
       61 acagcagcgg cctggtgccg cgcggcagcc atatggaccc cgtagtcttg agttacatgg
      121 acagtctact gcggcaatca gatgtctcac tattggatcc gccaagctgg ctcaatgacc
      181 atattattgg gtttgcgttt gagtactttg ccaacagtca gtttcatgac tgctctgatc
      241 acgtcagttt catcagccct gaagtcaccc agttcatcaa gtgcactagc aacccagcag
      301 agattgccat gttccttgaa ccactggacc tccccaacaa gagagttgta tttttagcca
      361 tcaatgataa ctccaaccag gcagctggag gaacccactg gagtttattg gtctacctcc
      421 aagataaaaa tagctttttt cattatgatt cccatagcag gagcaactca gttcacgcaa
      481 agcaggtagc agagaaactg gaggctttct taggcagaaa aggagacaaa ctggcctttg
      541 tggaagagaa agcccctgcc caacaaaaca gctatgactg tgggatgtac gtgatatgta
      601 acactgaggc cttgtgtcag aacttcttta ggcaacagac agaatcactg ctgcagctac
      661 tcacccctgc atacatcaca aagaagaggg gagaatggaa agatctcatt gccacacttg
      721 ctacaaagta gctcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      781 gaaaggaagc t
//
