LOCUS       sequence-227341-       11763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ClbK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Zha L, Wilson MR, Brotherton CA, Balskus EP
  TITLE     Characterization of Polyketide Synthase Machinery from the pks 
            Island Facilitates Isolation of a Candidate Precolibactin.
  JOURNAL   ACS Chem Biol. 2016 May 20;11(5):1287-95. doi: 
            10.1021/acschembio.6b00014. Epub 2016 Mar 1.
  PUBMED    26890481
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.6b00014"; journalName: "ACS Chem Biol"; date: 
            "2016-05-20- 20"; volume: "11"; issue: "5"; pages: "1287-95"
COMMENT     Sequence Label: pET28a-clbK
FEATURES             Location/Qualifiers
     source          1..11763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     966..985
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1005..1082)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1288..1307
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1391..1410
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1391..1409
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1410..1434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1449..1471
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1490..1507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1517..1534
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             8018..8035
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8088..8106)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8102..8149
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8186..8641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8273..8292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8483..8504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8733..9548)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8733..9548)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9456..9475
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9540..9559
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9670..10258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10159..10178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10412..10429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10444..10583
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10509..10528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10669..10691
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10685..10876)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(11685..11763,1..1004))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
       61 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
      121 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
      181 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
      241 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
      301 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
      361 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
      421 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
      481 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
      541 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
      601 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
      661 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
      721 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
      781 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
      841 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
      901 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
      961 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     1021 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     1081 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     1141 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     1201 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     1261 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     1321 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat
     1381 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     1441 gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
     1501 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg acttacagtg aaagcgatat
     1561 tgccattgtt ggcatgaact gccggtaccc cggcgtgcac agcgttgcgg cattcgaaac
     1621 ggtgcttcgc accggatgca acattttgga ccccaaagtg accccgagca acggccacaa
     1681 tcacattacg ctgaacaacg tctatgagca catggcggag ttcgatgcca acttttttgg
     1741 ctacagccgt gcagaagcag aaatcatgga tccacagcag cgcgtatttc tgacctgtgc
     1801 ttgggagatg tttgaacaaa gcggttacaa cccgaagcag cacgatgcgc gcgtcggcct
     1861 atatgcgggc gtgagtacca gcttctattt gcttactcac ctgatgaaca acccagacaa
     1921 gcttgcacaa ctcggtggtc tccaaatcat ggtcggcaat gacaaagatc atttgacgtc
     1981 ccaactggct taccgcctga atattaccgg cccttgcgtg acggttcagg ccagttgtgc
     2041 cacctcgctg gtggcagtgc atctggcctg cgaagggctg ctgagcggtc agtgcgatat
     2101 ggcgctagcg ggtggcgtca cgtttcgcat ggaggagcag cgcagctatg aatcgcacgg
     2161 tgatgggctg caagcagagg atggcctgat tcatactttt gatgcgcagg cgagcggtac
     2221 ggtctacagc agtggtttgg gcatggtgct gctcaaacgg gctacagacg cgcaggtgca
     2281 aggggataac atccttgccg tcatcaaggg aagcgccatc aacaatgatg gcggtgcgcg
     2341 cagtggttat accgtgccgg gtgttgatgg tcaggaagct gtgatgattg aggcgcatag
     2401 cttggcggag gtgacgccgc agcaaattca gtatctagaa ctgcatggca gcggcacgcc
     2461 gttgggtgac gctatcgaat tcgcggccat caaacgtgtg tttgggacgc cagcgccgaa
     2521 tgcgacaccg tggcgtctgg gggctgtgaa acctaacgtg ggtcacgtag aaatggcatc
     2581 tggcatcacc agtctcataa aaacggtgct gagtcttact aaccgggtgt tttaccctac
     2641 gctgaatttt caacgggcta atccgcaact ggggttggag gacagcccgt ttgaagtggt
     2701 gtcccgttta acgccttggc cggagggcac cacgccacgc accgctggtg tgagtgcgtt
     2761 cggattgggc ggcaccaacg cacatctggt ggtacaagcg ccgttatcga cgccgcaggc
     2821 gagggcgcag cagatggggc cttgtgtcgt tgtactctcg gcaaaaaacc acaatgccct
     2881 ggaacagatg caaaacgccc tgctggcgaa actggccgca catccagaga tacgcttaca
     2941 ggacgtggct tacaccctgc gtcacgggcg tttctccgcc ccggtacgta agtgtgtcat
     3001 cgcagagaat tgcacccagc tagcccgaca actccgcgac gcaccgatgg tggaggcaac
     3061 cacgggttgc acgatctact ggagattggg gcaccgtttc gttgttgcac tcgagacgct
     3121 tagtgactgg ctggcgtgct ctgaggtgtt gtcacaggcg gtgggacaac tgctcgagca
     3181 ttttccgctc gaaccagcct gtctgcaaga tctctcccct gcgcaacgga cgtttatcag
     3241 ccagtatgcg ctgatcgcgt tgattgacga acgggaaacg ttgaatgtgg tgctatgcgg
     3301 cgacggtgat ggtggctatg ctgccgccgt actacgagga gactgcacac tagagcaagc
     3361 atggcacagg ttgaatgcgg gccaaccgtt tgatgatgtg ccgacgaatc ccctcttgca
     3421 gcccgatgtc tgctctttga tgctggacga cgcggcgagt gatgccaatc gcaccgcgct
     3481 agaggcgtta gggcagttat ggttggctgg cgtcagccta gattggcgct gggtggatgc
     3541 ggcggagcgt atgttgggca gtcaacgcat cgccctgccg gggaccgttt ttacaccgca
     3601 gcgctattgg gtcgaggccg tacggcctgc cacgttctcc cacgaatcgt cgaataatct
     3661 cttatcccgc gcaacaaaat ccgatattat tgcagtggtg acggagatat gggaacgcac
     3721 gcttggtgtc agcattgatg atcaccacgc cagcttcttc gaactgggtg ggcattcgct
     3781 gttagcgtca accattttat atgatattca gcaacgttac ggcatcacct gtacgctaag
     3841 cgcattcttt gccgatccca ccatcgaggg attaagttgc tatctgctcg aacaaggcgg
     3901 cagtgagaca gcggtttcag cattgcctga tacggtcttt gcccctgacc agcagcacct
     3961 accttttccg ctgactgacg tgcaacaggc ctattgggtt gggcggcgaa aatccttggg
     4021 attgggcaat atttccaccc atatctatgt ggaatatgaa ctacaggggc tagatgaaac
     4081 agcgttcaac cgcgcgctca atgcggtgat tgcgcggcac agcatgttgc gtgcaattgt
     4141 caacgatgac ggtatgcagc agatattgcc aaacgtgccg gaataccacg ttgccttcta
     4201 taccacccag tgcgaggatg cgtttcaaca gcgttgccgt gagctgcgtg acaccctctc
     4261 acatcagatg attgattgta gtcgctggcc tctgtttcag atggaggtcg tggttgatcc
     4321 gcagcaaaaa gcccggctac atgtatctat cgacctgctg atagccgatg catggagtct
     4381 ggagttgttc attcgggaac tagcttatca ctaccgccat ccgcaggcgg cattgcctac
     4441 attgacgtac agtttccgcg actatgtgct gaccttaaaa tcctacgaga aaacgccgca
     4501 gtttgaacgg gcacgtgact actggcgcgc ccgcatcgaa actctgccgc cggggccacg
     4561 attgccgctg cgtaccgacc ccacgaagct ggaaaacccc acattcgtgc gccgtagcta
     4621 ttgtctatct cgtgctatct ggcagcgctt gaaaacgcag gcgggtcaga tgagtatcac
     4681 gcctactaca ctgctgctca cggggtttgc tcaggtgttg gcgcgcttta gttcctcgcc
     4741 gcactttagc cttaacctga cgctgtttaa tcgtctgccg ttgcacgcag atatcaatca
     4801 cttaattggc gattttactg cactgacgtt gctggagatt gatatgtcgc agggagaaac
     4861 cttgcaggcg cgggcaaacg tgatccactc tcaactgtgg cgcgatttgg ataaccgctt
     4921 gtttggcggt atccaggtct ctcgactgct ggtacaaact caccgcgatc cggcgaaatc
     4981 ggtgattcct atcgtgttca ccagcttgct caatcaatat gaggcgagct gggaaacgga
     5041 tgatacgctg ttcaaccaac cgcaggatga tctgtacagt atttcacaga caccgcaggt
     5101 gtggctcgat catcaggtaa tggagcgtaa cggggaattg cactttaatt gggacgtggt
     5161 ggaacaattg tttgaaccgg cgctaatgga tcagatgttc cagtgttatt gccaactcct
     5221 gcacgcccta gcccagcgac cacagctctg gcatgagact caagatgtgc tggcgctgcc
     5281 caccgttagt gcaccggtca cgcaggctcc tgcacctacg gccttgttgc accatgggtt
     5341 actgcgtcag gcagcactga cgccacagga aactgcgctg atcagtccta tccgtgaatt
     5401 gacctatcgc caactgtcga cggcggcgga tcatgtggcc cgcgccctgt tagcgctggg
     5461 cgtgcagcat ggcgaccgcg tggcggtggt gatggaaaaa ggctggcagc agattgccgc
     5521 cgtacacggc attttacgac tgggtgcggt ctatctgcca gtggatccgg tgctaccgcc
     5581 acagcgtcgc cagcttttgc tgacggtggg cgaggtgcgg gtacaagtaa cgcagccggg
     5641 tctcacgcaa ttggagccgt cgctgcccgt gctgatcatc gacgacggaa tgctggacac
     5701 gcctgctgcg ccgttgcctg aagtggctgg ggatgtcacg gatctggcct atatcatttt
     5761 cacttccggc tccaccggta ccccgaaagg agtgatgatc gaccaccgtg cggccatgaa
     5821 cacgctggaa gacatcaacg aacgctttgg cctcaatgcg caggataggg tgttcgggct
     5881 gtcatcattg agctttgacc tgtcggttta cgatgccttt gcgcctttta tggtgggtgc
     5941 agcgctggta ctgccggaag caggacggga aaaagatccg cgtcattggc agacagttat
     6001 ggcacacggt catgtaagcg tctggaatgc agtgcccgca ctgatgcaga tgctgtgcga
     6061 ataccacagc ggcgatcgga tgagttatcc gacgttgcgt ctggcactgt tgagcggcga
     6121 ctggatcccg ctaacgttac cggagcagat gcgcgagcgg ctcaatgaaa cgatggacat
     6181 catcagtctg ggtggagcga ccgagtgcgc catctggtcg gtctactacc cgataggtga
     6241 ggtggaatcg acgtggacca gtattcccta cggtcggggc ctgcgcaacc agccagtata
     6301 cgtgctaaat gcgcaactgg aggaatgtcc ggtcggggtg gaaggagaga tttgcattgg
     6361 cgggatgggg ctggcacaag gctacctgaa cgacgcagag aaaacggcgg cgagctttgt
     6421 ctggcgcgaa gcgagtggtg agcgaattta ccgcactggg gatcgcgggc gctactttgc
     6481 tgacgggcaa gtcgcctttt tggggcgcaa cgatacccaa gtgaaggtga atggttaccg
     6541 tatcgaactg ggggaaatcg agcgctgcat tgcgcgacat cccgatgtgg agcagtcagt
     6601 ggtggtggca gtgggtaatt ctcaacatcg tcggctggtc gcttttgcca aactgcacga
     6661 tcgccaccag gcgcaggcat tgcaagctaa ggaagcggag gcggcggcac tggcgcaggg
     6721 tattattgtg aatccggcac agcgtctagc gttcaaactc aaggagccac atattcgcgc
     6781 gctggatggt ctgggcattg cactgacggc accggcggat agcacacgtt acatcaaacg
     6841 ccgcagctat cgtcatttca gcgcgcaaaa aaccacgctg gcacagttgg ggcaattgct
     6901 gtcgggcttg gggcagatgc gtctacccgg tctacctttt gccaagtatg cctatgcgtc
     6961 cgccgggggg ctatacccgg tgcaaaccta cgtgtacctg catccagaca agatcgaaga
     7021 gggagtatcc ggtatttact acttcgaccc gcgacagagc tgtcttatgc cggtagcacc
     7081 agaagtcgag ctgaacagtg gttttcatgc cggacctaat cagtctattg ccgatcgggc
     7141 ggcattcacg ctgtttatgg tggctgatat ggcggtgatc tcgccattct atgggcaaga
     7201 ggcagcttgg cacttctcgg tgatggaagc aggtactctc tgccatttac tggaagaaga
     7261 tgcgccgcgc tacggattgg ggctgtgcca acttgggatg gcagactttt ccgctgtggc
     7321 atcgcatttt caattgtcgc cacatcatcg ctatgtccat tgcaccgtgg ggggcgcgat
     7381 agggcaagag gcggcaagtg ctgcagcatt gctgcgcgat ttctccacct atgagaaacc
     7441 gaaggaaacc gctgcgccgc tggacatgca gagctacaaa gatgccatgc tgcgcggcct
     7501 gcgtcagcaa ctgcctgact atatggtgcc gagtgatctg atgttagcga ccgattttcc
     7561 gttaaccgct aacggcaagc tagatcggca aaaattacag ctgcagggcg aacaaattgc
     7621 ccaccagcgt gacggcgtgg gtccaatcca ggtggacagt gcgttacaac agcggctggt
     7681 ggcgctctgg caggaggtac tcggcgtgag ccacgtgtcg gccgaagacg atttcttctc
     7741 gctggggggc agttctatag aattggtgcg tattcagcag gcacttgagg cgattatcgg
     7801 gcaggagatt cccattgtcg atctgttccg tctgccaacc atcgcggatg tggcgcgtta
     7861 ccttgatgag caactgcaca atctacccgc cgcccacgat atcgtattag cgcaggctga
     7921 ggtttcgcag gtcagcgctg cgcgcgagaa tctggcgttg cggcgtaaac gtgcccaaca
     7981 gggagaaaag ggcgatgagt gagcggccgc actcgagcac caccaccacc accactgaga
     8041 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     8101 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     8161 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8401 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8521 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8641 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8701 attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga
     8761 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     8821 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     8881 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     8941 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     9001 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     9061 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     9121 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     9181 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     9241 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     9301 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     9361 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     9421 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     9481 aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata
     9541 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac
     9601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9781 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     9841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10321 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10381 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10441 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
    10501 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
    10561 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
    10621 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
    10681 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
    10741 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
    10801 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
    10861 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
    10921 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
    10981 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
    11041 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
    11101 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
    11161 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
    11221 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
    11281 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
    11341 gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
    11401 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
    11461 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
    11521 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
    11581 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
    11641 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
    11701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11761 gtt
//
