LOCUS       sequence-227343-        6706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            bb0826 promoter driven mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2_P0826-mCherryBb
FEATURES             Location/Qualifiers
     source          1..6706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1007..1024)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1007..1023)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1016..1038)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1590..2399
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1657..1676)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2568..3156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3057..3076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6633..6654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6669..6674,6675..6692,6693..6699)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattcgagct
       61 cggcaataga agaatctata gaaagcataa aaagccttat agaaaacaaa gaatacatac
      121 aaaagttaca ttttataaaa aaattaataa ataaggttta caaaaaatta aattactttt
      181 tttaaaaact aaactttgaa agccttgtta taatataaaa tataataatc aaatattatt
      241 caaagttaac agcaatgaag tttataataa attggatcca ggaggttcat ggttagtaaa
      301 ggtgaagaag ataatatggc tattattaaa gaatttatga gatttaaagt tcacatggaa
      361 ggtagtgtta atggtcatga atttgaaatt gaaggtgaag gtgaaggtag accttatgaa
      421 ggtactcaaa ctgctaaatt aaaagttact aaaggtggtc ctttaccttt tgcttgggat
      481 attttaagtc ctcaatttat gtatggtagt aaagcatacg ttaaacatcc tgctgatatt
      541 cctgattatt taaaattaag ttttcctgaa ggttttaaat gggaaagagt tatgaatttt
      601 gaagatggtg gtgttgttac tgttactcaa gatagtagtt tacaagatgg tgaatttatt
      661 tataaagtta aattaagagg tactaatttt cctagtgatg gtcctgttat gcaaaaaaaa
      721 actatgggtt gggaagctag tagtgaaaga atgtatcctg aagatggtgc tttaaaaggt
      781 gaaattaaac aaagattaaa attaaaagat ggtggtcatt atgatgctga agttaaaact
      841 acttataaag ctaaaaaacc tgttcaatta cctggtgctt ataatgttaa tattaaatta
      901 gatattactt cgcataatga agattatact attgttgaac aatatgaaag agctgaaggt
      961 agacatagta caggtggtat ggatgaatta tataaataag cttggcactg gccgtcgttt
     1021 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1081 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1141 tgcgcagcct gaatggcgaa tggcgcggcc gccctaggtt aatacccgag cttcaaggaa
     1201 gatttcctat taaggttgaa cttaagagct taagcataga cgatttgaaa aaaattttaa
     1261 aacaaacaaa aaattcttta ataaaacaat atgttgcgat gtttaaggtt tatgatttag
     1321 atttaaagtt tagtgaggaa gctatagata gaattgcaga gcttactttt aatatgaatc
     1381 ttgagagtga aaatcttggt gccagaagac ttcacggtgt tatggaaata gtgcttgcag
     1441 atcttttttt tgaagtgcct ggcagtaagt tgaaaaaatt tgaaataaac ttggactatg
     1501 ttaataaaaa aatacaaatt aacgaacaaa aagatttgaa ttattatata atttagttaa
     1561 aagcaatttt aaatgaggga ggtttccata tgggccatat tcaacgggaa acgtcgaggc
     1621 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1681 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1741 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1801 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1861 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1921 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1981 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2041 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2101 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2161 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2221 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2281 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2341 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2401 cagactcgag tggccgagga gcaggactga cacgtgctaa aacttcattt ttaatttaaa
     2461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2641 ttgccggatc aagagctacc aactcttttt tcgaaggtaa ctggcttcag cagagcgcag
     2701 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcact agatccccgg
     3241 caagttcatc cttagggttt tcatatctct taaaaagtca atttctgctt gattaatttc
     3301 ttgtggatta ttgtttttgc ggtttttcat tttttatctc cttgagtatt gaattttttc
     3361 ttatatataa atatatagca aaaactattt ttgccaactt ttttacaaaa aattttttat
     3421 taaaacactt agggctttgc taaattctct tttaaaagaa cttagtaaag ccctatggat
     3481 ttaagaactg attatactta cgtagtaaaa tactacagat attaatagta tagaccatat
     3541 caaaatcata atcaatttat atttatctaa aattgtagtc catacaaaat acaaaaaaat
     3601 taggtttttc taaattctat tttaaaagaa cttagtaaca cctaatctct aaattactta
     3661 caaaattcta aaattagatt tttagttctt catatttctt taaaagtttt ttaagaaaat
     3721 ctttttgatt ttcaaaaatt tcatgcatca taaaactagt aaatttggat ttttttttgt
     3781 aaaaatcata actttcttgg gttttaagtt gaaatcttaa tggttttatt ggattttgcc
     3841 tagatttttt taacgccgga ctttctttat cttttataaa attataagaa tttttaatac
     3901 cattttctat taaataatct tcttttataa gcccctcttg cagtgcagtt gctaatctta
     3961 aatacttata cgcttgagtt tttgctacat caaagtcttt tataaaatca agaaaagttt
     4021 tataaccatc aaatttataa tattgattat cttttatttc ttttaaaatt ttaatatttt
     4081 ccaccctgtg gaatatttct tttttgaaat tgtcatttaa cttttccttt agtgtcttgt
     4141 aataagaaat tttactttca ttaatcatta aattttgttc aacataagta tctatctttt
     4201 ttgttaaaac tttaacaaca cgtttttttt ccataaaaat tttcctttat ttttgcaaca
     4261 ttttccttaa ttgatataat tgttactatt ttagattttt taatatccac ttaagaatta
     4321 atccctcact agaatatttt ttattataga attcatatac atgtttttct aaatctataa
     4381 atttatcaac aattgcttta ttatagggtg tatctatctt ttcttttctt aataatcgtc
     4441 tcgtattctt taaataacaa caaacactgc cggttttaaa cctaaattct atgtaatata
     4501 tttttgaaaa agtatataca ttttcaaccc cgtttatttc atactttaca aaaacattat
     4561 ttattggttt tctataacca tagtaaatgc ctaaaaattt gtccccgtct ttcataggat
     4621 atagataaag tttttgaatc tctgatttat ctaataattt ttcaaaaaaa attatacacc
     4681 gcttttgttt gttactaact actctatgca atgcctttaa tatttttgtg tgatatatat
     4741 ttttatcacc ttttttttct ttcagcagga agatactttc ttttggaaat ttaatttttt
     4801 gttcatttat tcttgcagcc ttaagtccga ttttcatatt taatccttat atagcctttc
     4861 tatttaattc ttttaaactc aataaacttt tttggttttt cattagttct aataattcgc
     4921 aataatatgt gcaaaacact ttattgtatt ctattttctt ttggttattc aaatattctt
     4981 ttataattgg ctttagaagt tcaatattta cttctttttt tagttgttca ataaggatat
     5041 tgaaaatgtt tgtttttaaa tcttgataat tttgttgaga attttctttt tttctttcaa
     5101 ttgacttctc taatttacgc tttatattgt ttaaatcgct atatttatga ctttcaataa
     5161 taaaatgagg cttaaatttg taattttcgt atattttttg gaaacttgtt tctaattgtt
     5221 tagaattata ttcatttttt tcaaattctt tttgagtgtt gcatagaatt tcctttaatt
     5281 tttcttgttt ttctttcata caagatttag taaaatgtat atttttattt tttagtaatt
     5341 taatttctat tatttttatg attttaagta ttttgattat attatcttta tcaatattta
     5401 aattgaataa aattgtaaga aaaattgaaa gaattttttt acataaaaag ttacatttat
     5461 tgaagtaatt tcttacttga tacttttcta tttcattaat ctttctttct tcttttatat
     5521 tatttttatt atttaaacac tcctttaaat ctacactccc atttttagta aatctatctt
     5581 taaggcccct tgaaactcta ttttgaaatc ttaaatcttt tttttcttga aagtatttat
     5641 agattttttg gtaacattct ttttttgaaa aattaagttg atagtatatt tcggtcccca
     5701 aatttacccc taagtgtttg tgataattag ttgtgacttt aaattctttt tctaatttgt
     5761 aaaggtaatt ttgcagtgtt tttagtttaa cgggtgtttg actatttctt ttaaggtttt
     5821 cattaaagta atagagtatg tttttttgtg tgtatttctt atatgtttta tttatgtacg
     5881 ctagtgttga gataaggact actaatttat actgtaattt attgtagcaa gttgtggttt
     5941 tactggtttt gtttatatta gataagtttt ccatttatag ctccttgaat accggatttt
     6001 ttatctataa agtatatata tcacaatttt gatttaaaag taaattcttt ttttaaaaat
     6061 acaaaaattt tttataaatt atatagacca tgatattgta ttaatactaa ttgattttaa
     6121 tttaaggttg aactaaaata aacatagttt tgtagaaaat aatttttcat tattttgcta
     6181 cttgatattg gatcgtaaaa atattagggc tttacaaagt tcttttaaaa gagaatttag
     6241 taaagcccta agtcttttaa taaaaaattt tttgtaaaaa agttggcaaa aatagttttt
     6301 gctatatatt tatatataag aaaaaattca atactcaagg agataaaaaa tggaaaactt
     6361 aaacaataat ccacaaaaaa ttaatcaaat aaataagcaa actaatttta ttggttttga
     6421 aaattttata gaacttgata atcaagttaa tcaaactaaa gaaaaaagta aattaagtaa
     6481 gataggtaag aaattacccg gaataagcag tcaagtctag tgcggccgcg cccaatacgc
     6541 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6601 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6661 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaa
//
