LOCUS       sequence-227344-       10162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length ClbH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus 
            EP
  TITLE     Colibactin assembly line enzymes use S-adenosylmethionine to build a
            cyclopropane ring.
  JOURNAL   Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 
            10.1038/nchembio.2448. Epub 2017 Aug 7.
  PUBMED    28805802
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2448"; journalName: "Nat Chem Biol"; date: 
            "2017-10"; volume: "13"; issue: "10"; pages: "1063-1065"
COMMENT     Sequence Label: pET28a-clbH
FEATURES             Location/Qualifiers
     source          1..10162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     374..393
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(413..490)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     696..715
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     799..818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        799..817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    818..842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             857..879
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             898..915
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             925..942
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             946..978
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             5825..5842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5895..5913)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5909..5956
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5993..6448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6080..6099)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6290..6311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6540..7355)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6540..7355)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7263..7282
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7347..7366
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7477..8065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7966..7985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8219..8236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8251..8390
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8316..8335)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8476..8498
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8492..8683)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9492..10162,1..412))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
       61 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
      121 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
      181 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
      241 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
      301 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
      361 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
      421 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
      481 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
      541 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
      601 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
      661 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
      721 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
      781 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
      841 cccctctaga aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
      901 catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgact
      961 ggtggacagc aaatgggtcg cggatccgaa ttcatggaac agcaagggat tatgagacag
     1021 ttgcctaccg acgaccaaac gatagtcgac tatttgtatc gtatcgccgg agaatatggg
     1081 gaaaaagccg ctgtattgat gggggacgcg gcgctgagct atcacgatct taatgcacgc
     1141 tctaaccaac tggcgcatta tctgcgtggg ctggggatcg gcgaggatcg tgtggtagct
     1201 atccgcctgc cgcgcggcat ggcaatgctg attgccattt tcgctattgt aaaagctggt
     1261 ggtgcctatt taccgctggc gtacaatgca ccgcgtagcc gcattgaaaa tatactgagc
     1321 aacagcggcg ctgtttgtct gatcggtact gacgatggtg atcgctggcc gattcctcgc
     1381 gtcgaaatcg acagcgcggc ggtcagtgcc atgccaacga cggatctgcg ttaccgacca
     1441 catgcgcggc aactggcgta tattatttac acctccggtt ccaccggtgt ccctaaaggg
     1501 gtggcgacgg aacatgcagc gctgctaaac cgaattgtct ggatgcagaa cgcttatcct
     1561 atcagttctc aggacgtgct gtttcagaag acggtgtaca cctttgacgt ctctgtctgg
     1621 gagatgttct ggtgggcgat gtacggcgca tctgtagtgc tgttaccgtc cggactggag
     1681 agcgatccgc gaaccttggc tcgactgatt cagcgtcacc gcgtgtcggt tgtgcacttt
     1741 gtcccttcga tgctgaacct gtttgtcgag tatctggaga tgaaacagga tcctcgtttg
     1801 accgcctcat tgcgattagt gttttccagc ggtgagaaac tcacggtcca cagtgtggct
     1861 cgcttttatc aatcggtggc gcagggtgat cttataaatc tctatggccc gaccgaagca
     1921 gcaatcgatg tcagtcatca ccgctgcctg cgcgggtacg actacgacga tatccctatc
     1981 ggtcaagcga ttgacggttg ccgactctat gtgctggatg accatggtaa tccggtagca
     2041 gacggcgaag agggcgaact gtatctcgct ggtatcgggc tggcacgtgg ctatctcaac
     2101 aacgtggcgt taactgatcg ctgttttact atacatccaa ctttgcgcca tttagggaaa
     2161 ccggagcggc tgtataaaac tggagatctg gtgtggcgcg acggggaaag ccaacaaatt
     2221 cattacattg gccgtaatga ttttcagatt aaaattagag ggttgcgcgt tgaattggga
     2281 gaaatcgaag cccatgcgat gcgtttcccg ggggtacagc aggcagtcgt ggtggcggat
     2341 caggatgatc ccgacaatca gttgatttac gcttttgtcg tcagtagcgt gccgctcaat
     2401 ttggcggcct taatggacgc actgtccaaa aacttgcctg cctacatgct gccgaaccgt
     2461 ttgttggcaa tgtcagagtt accactctcc gacaatggca agtgctgtcg taaaacgttg
     2521 ctcgacttgg cgcgggcgta ctcagccagt cgcgtcgatt tacgtgaaac tcccgccgtg
     2581 cgctacctac cgttgtcatc ggctcaatcg tcgatgtggt ttatgcaaca attggcgccg
     2641 catactgcac tatacaataa ccctaccgcc ttgctgcttg aaggagaact ggatcgcacg
     2701 cggatggacg gcgcgattcg tcaattgatg agtcggcata ctctgttacg tgctatggcg
     2761 gaaacccaca atggacaacc agtattggcg gtgcctcagt gcgtatcgtc gcaggcgttg
     2821 ctgaccatag tgccactccc ctcggtgagt gatgataacg cgctacaggc gatgatcaac
     2881 cagcgtgcgg cacaccccat gcccttaacg tcaggcacac cgctgtgccg gtttgaactg
     2941 ttgacgctcg atgacgatcg tagcgtattg ttgattcatc tgcaccacat catcagtgat
     3001 ggctggtcga aaggcgtttt gttacgcgaa ttgcaggccg catataacgg tgaatcgctg
     3061 acgccagaac cgttgttgga gtatgccgac tacatggagt accaagaaga gtggcgtcag
     3121 agtgacgcct atcaggacgc gatgcgttac tggcaaaata ccctggcggg gacgttgccg
     3181 attcttgata tccccaccga tcaaccgcgt cagaaggtgg cacgttacca aggcgcgttt
     3241 gttgccttcg cattgtctgc caacacatgc gagcgagtgt tggcggcggc gcgtgcgcag
     3301 cgcgtgtcgt tgtataacta tctgctgacc gccttcgtcc tcctgctgca tcgtaatgcg
     3361 cgtcaacagg aatacatcgt cggtatgccg attgctgcgc ggctgacgaa agaacaggag
     3421 catatgatcg cgccgctggt caacgtacta ccgctgcgct tacctcttga cgaagcggca
     3481 tcgttttccg agttagtaca gacgatcaga ggtattctgt ttgccgcttt caggcaccag
     3541 cgtctcgaat ttaccgacat agtgcgcgca gtgaatgtgg atcgcagcgc cggacatttc
     3601 ccaatatatc agtgcatgtt ccaactcgac aatatgcctc tggcgagccc gacactaaac
     3661 ggtgtcaacg ttacaccatt attgttggat accagtgcat ctcaggtgga tatttcgcta
     3721 agtatgcagc atatcgatgg gcgcatcacc ggcactttcg aatacgacgc tgggttatac
     3781 agtgcggatc gtattcagca cctggtggcg cagtggagag aactgctaga tgaggccagc
     3841 agccaaccca cgcagttagt ccgcgatttg attcgtttca cccctcgaga gcacgcttgg
     3901 ctggcgcggc acaacgccac tgaagttgct ctgccgcccg tagataacct gctggcgcta
     3961 gtgttaccgc actgccagca gcgccctaca caggtggctt tgcgccacgc tgacgacgcc
     4021 atgacctatg gcgaattgca acaggccacg atgcagatgt gtacttggct gcgtgcgcaa
     4081 ggcgtaaaac gcggagaatc tgtcgcactg caactgcctt tttgtttcga attgattatc
     4141 gcacaattgg cgatcctctc tttgggggcg agctatgtgc cgctggatgg taacgcgcca
     4201 gcggcccgca atgccctgat cttggcgcag gcaacgccgt gcatgctgct ggtggcgcaa
     4261 ccgctggaat cccctcatgg gctgacgata ccttgggtgc tagtgcctga ctggcgtagc
     4321 ctgctgacag aaataccgaa cctgccagtt agtgttgcgc cagatgcttt agattgcgat
     4381 gcggtggtga tctttacctc cggtactacc gggcagccca aaggcgtccg actcagccaa
     4441 cgtaatctgg tcaacttaac cgcatccttt atatccagct atcaagtgac gcatcaggat
     4501 gttctgctac ccattacctc agtcgcgtct gctagctttg tcggggaggt actgccgctg
     4561 ctcgccgctg gcggtacctt agtattggcg caaaaagcgc agagtttgga tagtgatgcg
     4621 ctcattgcgc tgctggcatc tcagcgggtg actatcctga gcaccacccc atcgctttct
     4681 gccagcctct ctgtgctggc tcagtcgatg ggatcgctgc gcttgtttct gtgcggtggt
     4741 gaagcgttag agtatgagca gatagcgccg cttctgccgc acatggcagt ggttaacggt
     4801 tatggcctga ccgaaagcgg tatctgctcg acctactttc ctgtcgcaaa gcgtagggag
     4861 caagaaacgg gagcattgcc cattggccgc ccgattcaga acacccaagc ttatgtggtg
     4921 gatgcatata atcgtttggt accgccaggt gcctgtggag aactctgttt ctctggtctg
     4981 ggcatttcgc ccggctacct tgatgcacga caggatcccg agcgctttgt cgagttgccg
     5041 gaataccctg gcgttcgggt gctgaaaact ggcgatcgtg ctcgttgggc aaccgacggg
     5101 atgttgttct atctcggcag acaagatcgc caagtgcaga tccgtggata ccgagttgaa
     5161 ctgggcgaca tcgaaagcct actgaaacag catccggata ttgctgatgc gtgggtcgat
     5221 gtgcgacgca atgcggcggc aacgccgcta ctagtggcct tctattgcag cgtcaacggc
     5281 gttgcgctgg atgctcagca attgcgcgta tggctcagcc tgcggcttcc gttgcatatg
     5341 ctgccgttgc tctacgtgcc gctgagcgcc atgccgctag gtgtaaacgg caaaatcgat
     5401 ccccagtgcc tgccgctggt cgatctgcgg cagttggaag ggccgggcga gtatgtccct
     5461 ccggcaaccg aactggaaca gcgtctggcg gagatctggc agcagttgct tggcctggag
     5521 cgtgtcggca ccaccacaaa tttcttcgat ctcggtggac actcacttct gctagtacag
     5581 atgcagcaat acatcgggca gcagtgcggt cagcacgtgg cgttggttga cctgctgcgc
     5641 ttcactacca tcaaacgctt ggcggaattt ctgctggccc cggacgcagc gcaagggacc
     5701 acaggagatc agacacaact gcgtgcggcg aagcagcgtt tggcctttgg tcacacgcgc
     5761 tgggcagcca caaccgacag tcatcactga gagctccgtc gacaagcttg cggccgcact
     5821 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
     5881 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     5941 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
     6001 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6061 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6121 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6181 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6241 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6301 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6361 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6421 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6481 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct
     6541 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     6601 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     6661 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     6721 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     6781 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     6841 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     6901 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     6961 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     7021 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     7081 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     7141 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     7201 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     7261 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     7321 ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     7381 taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7621 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8221 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8281 gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8341 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     8401 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8461 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8521 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     8581 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     8641 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     8701 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     8761 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     8821 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     8881 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     8941 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     9001 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     9061 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     9121 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct
     9181 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
     9241 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
     9301 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
     9361 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     9421 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     9481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     9601 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     9661 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     9721 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     9781 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     9841 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     9901 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     9961 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
    10021 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
    10081 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
    10141 gccggaacat tagtgcaggc ag
//
