LOCUS       sequence-227346-       14985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length ClbB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus 
            EP
  TITLE     Colibactin assembly line enzymes use S-adenosylmethionine to build a
            cyclopropane ring.
  JOURNAL   Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 
            10.1038/nchembio.2448. Epub 2017 Aug 7.
  PUBMED    28805802
REFERENCE   2  (bases 1 to 14985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2448"; journalName: "Nat Chem Biol"; date: 
            "2017-10"; volume: "13"; issue: "10"; pages: "1063-1065"
COMMENT     Sequence Label: pET28a-clbB
FEATURES             Location/Qualifiers
     source          1..14985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     961..980
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1000..1077)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1283..1302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1386..1405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1386..1404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1405..1429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1444..1466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1485..1502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1512..1529
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1533..1565
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             11235..11252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11305..11323)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11319..11366
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      11403..11858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11490..11509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11700..11721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(11950..12765)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11950..12765)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12673..12692
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12757..12776
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      12887..13475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13376..13395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13629..13646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13661..13800
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13726..13745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13886..13908
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(13902..14093)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(14902..14985,1..999))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
       61 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
      121 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
      181 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
      241 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
      301 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
      361 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
      421 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
      481 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
      541 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
      601 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
      661 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
      721 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
      781 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
      841 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
      901 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
      961 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     1021 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     1081 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     1141 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     1201 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     1261 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     1321 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc
     1381 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
     1441 aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
     1501 acagcagcgg cctggtgccg cgcggcagcc atatggctag catgactggt ggacagcaaa
     1561 tgggtcgcgg atccgaattc gagctccgtc gacaagcttg cggccgcatg gataatacct
     1621 ctggagattt tccatgtaat aagatggaca cgcgtaagca gttaccgcta acaccaagtc
     1681 aacaggggtt tttattccat tccttaaagg ataagaaaag gagtaactac catgagcatt
     1741 ttacatgcat tttttctcag catgtagata gcgcccactt caagtgggcg ctggaaacgt
     1801 tatttcgaaa gcatgagtgt tttcgcactg attataactg ggagattgat gagcgccctt
     1861 gtcaggtggt gaagaccgat gtgttgccgg atatatatgt gttagactgt gagcaagagg
     1921 aaatacgttt tctactagct aatgatgaca ttatcattcc tgtcccgcag gatgacggta
     1981 ttgatgctat aattcctcaa ctgctacagg ctgatttaaa atacccattt tccttgaaaa
     2041 cgatcccagt ccgggcctac cttattcagt caacgaaaga aagtgctttt atactatcat
     2101 accatcatat tgtgatggat ggctggagct tatccctttt cattaaacag ttgctccaac
     2161 tctatggagc ggctgtggtc agtggtgtga gggatgatag cgccattatc ccctcatctc
     2221 tgaaacccct tgtagacaca ctgtcggccc gacgtcacac ctttcagcac gactattggg
     2281 ctgcatatct tcgggaggga acaccaactt gtatcgtgcc gctgtcacaa tatcacacag
     2341 atactgaagc cgagaacaat tcttacgtta atcaaacaaa tcatgtggag atcaatttgt
     2401 ctccggatgt gtgtcagaaa atacagacgc tatgcagcga ttatcgtatc acccccgcag
     2461 taatcttcta tgtggcctgg ggcatcctgc tacaacgttg gtgctatgct gacgatgtgt
     2521 tattcggcgc gacaatatca gggcgaaata taccaattga tggtatagaa gaaacactag
     2581 ggctatttat taacacgttg ccgctgcgtc tgcgtgatga tggggcgaca ctgttgcaac
     2641 acttacaacg tatgcaccaa acactaatag ctcactacag caatgaacat gatgccttag
     2701 ccagcataca acggttggta cataaagaag gtcatgctgg ggatcttttt aataccttag
     2761 tggtgttgga gaattatccc gttgatatga cattattgtc atgcgcgtcg cccgtggcaa
     2821 ttcgccatct cagtgtacat gaacaaacgc actatccgct gaccttgacc atcactcaac
     2881 agaagggatt ccgtttcagt attgcatatg cccttaacta cctgaccaac aacatggcgc
     2941 aagcgttgct gatgcacctg agttatctgc ttgaacaact ggtggacaat ccgcagcgcc
     3001 ccattgctgc gttggtaaac ttgtccccat gccagcaggc gcaggtactt caaccctatc
     3061 tggaacgcat ggcatgccgg gattgggata gtcaatccaa cgtcatcgaa caatttcatc
     3121 aagttgctgc gacttcgcca gcacaggtcg cagtggttga tgaattgtgc gcgttgacct
     3181 attcggagtt ggcagcacaa gctgagcagc tcgcggctta tttggtacag cagggtgtta
     3241 tggttggcga tactgtaggc ataattagcg aacgccgggt aaacaccgtg gttgccatca
     3301 tcgccatcat gttgatcggg gctgcttatg tgcccatcag ccccgactac ccagtgggta
     3361 ggatgcagga aattattgat gacagcggct tggcgctgtt gctggtacat ggcaaaccgc
     3421 tagatgcatt aaacgttgcg cagagtgacc tctgtgcatt tcccgtcgcg ccctcggtgg
     3481 tatttccggt tatcacacca gattctcgcg cttatgtgat ttattcatcg gggtcgacgg
     3541 gtaagccaaa aggtatcgcg gtggcgcacc gcggtttatt gcgcctgata caaggcgaca
     3601 gcccgctgaa ggtggagagc ggtgagacaa cgctgctgac ctgcccattt gagtttgatg
     3661 tttcggtgtt tgagatgtgg tctaccttgc tcaaccacgg caaactagta ttactcagca
     3721 aacaggcatt acttgatatc aatcacattc gccgcacgat cgctgatgaa caggtggcgc
     3781 gcgcctggtt tacctcatcc ttgtttaact cctatgtggc agaaggtgcc gatttcttcg
     3841 gtatgttaca acacattacg gtgggcggcg aagcagtatc tgcgtggcat gtcaacgacg
     3901 tgatgcaaaa atacccgcat ctggtggtga caaacggtta tgggccgacg gaaaacacta
     3961 tttttaccac cgcatatcgt ttcaacgggt tgcaacccgc ccgagtcccg ataggatacg
     4021 cggtaccggg cacctcgctc tacattaccg atctccatgg gcatttgttg cctatcggtg
     4081 ccaccggtga actagtggcg ggtggagtgg gggtcgccat cggctatcag aacaacccgg
     4141 cgctaagtgc gacggttttt gtccctgatc cttttattcc cggcggtatg atgtacaaaa
     4201 ctggcgatta tgcccggttg ctggatgatg gctgtgttga ctgttttggg cgtaaagacg
     4261 gtcaaattaa gatcaatgga caacgaattg aaaccggaga gatcgagcag cgcctgctgg
     4321 agtgctccgg cattatcgag gcggtagtgg ttccttaccg cgtacgtgaa acgctgcata
     4381 ttgcggcagt ggtctgcgtc aatgatagct atgatgaggt ggaagttcga gggcaattgg
     4441 ctgacagatt gccgccattc gctattccgg aatccttggt ggtggtgacg gagattgcaa
     4501 aaagccatag tggcaaggcc gacttggcgc agttgcggta tctcctgccc gcaactcagt
     4561 gcaacgccgt gtccaccacg atatcagagg tgcatagtga catggaacat gcgctgcatg
     4621 ctatctggca acgcgtactt gatagacaag acattgatag caatgcctcc tttttcgccc
     4681 tcggtggcac ctcattggat accatcaggg ttaaagggga tattaagcgg caacttggct
     4741 tggagattga tattaccgat ctctttaagt acccaacgct cacggcgtta gcacattttc
     4801 tcgatactgc cgtatcgccg gaggatgcaa ttccaacgcg tgctgttgtc tacagcgaca
     4861 tgccggtggc gattgtcggt atggcgggac gtttccccgg tgcggcgaat attgcagcgt
     4921 tgtggacgct ggttgtagga ggggaatcgg gattaacact gttcagtgat gaagagttgc
     4981 gcgcgcatgg tgtgacacct gacacgctta aacaagcgaa ttatataaaa accaaaggga
     5041 ttgttgatga tcacgaatgg tttgatgcgg atttctttgg ttatactccc aacgaggcgg
     5101 aatgtatgga tccgcaaatt cgcttattgc atcagtgctg ctggcaaact ctggaacacg
     5161 ctgggtgcga tcctgccacc tttactggtg cgattggcat ttatgccgga ctgctgacat
     5221 ccccccactg gcttaatgcg gtaatgcaag acactaccga ctctaccgcc ctgtacaagg
     5281 ccagtatcct gaatatccat tccgtcacag cattgattgc ccatgcgctt aacctcaccg
     5341 gccctgccgt gacgcttgac accgcctgct ccacttcggc agtggctatc caccaggcct
     5401 gcatcgcact acgtaaccgg gattgcgatg cggcattggc gggcggcgtt tctatcgaga
     5461 tgcctgcgta ccggggctat gaatatcatg aaggcatgat caatgcgcga gatggtgtct
     5521 gtcgtccttt cgatagccaa gcctctggca cagtcaccgg tgatggctta ggaatgctgc
     5581 tgctaaaacg tctggacgac gcgctggcgg atcgggactg catctatggt gtgatcaaag
     5641 gttcggcggt caataatgat ggcaataaca aaatcggcta taccgcgccg agtgtgatcg
     5701 ggcaatcaac ggtaatccgc accagtttgc gccgtgccgg ttttgatagc gacagtatcg
     5761 gattggtgga agcgcacggc accggtacgg tgctgggtga tcctattgag ttacgtgcgc
     5821 ttaatgaggt gtttggccca acacctgttc cgttttgtgt ggtttcagcg ctgaaaagta
     5881 atattggcca cctcaactcc gctgcgggag tggcgggcgt catcaaaaca acgctcgccc
     5941 tgcatcatca agtgttgcca ccaacagcgc actttcgcca actcaatcct gctatagatc
     6001 tatcgcgttc tgcattgtac gttaaccagc aagtccaacc gtggccgtcg acgcgtcctc
     6061 gtcgtgcact ggtgagctcc ttcggcatcg gcggcaccaa tgccagcatc gcactagaag
     6121 cacatcaaca tgaggacgac ccttcagcga cgggggtacg cgacagctat ctgttgttgt
     6181 tctccgctaa aacacccgct gcgttagagt tgcgcgtggc ctccacactg gaatatgtca
     6241 agcatggagt aggggtgcgc ctgccggatg tggcttatac attgcaaact ggacgcacag
     6301 cctttgacca tcggcgggct tatttggtga gtcgtgggtc gaaaatcgat ctctcctgtg
     6361 ccacgatatt gcaagcggaa atcttcaatg gtcagcgcac gacagcggag atctgcttca
     6421 tgtttcctgg tcaaggtagc caatatcacg gcatggccag cgcgctctat gctcatcaac
     6481 ccatgtttcg ccagcacatg gatcgctgct ttgctgcatt ccaacgctat tcgacggtcg
     6541 atctcaaggc gttgttgttt gacgatgagg atacgcggga tattgatcaa acgcagttca
     6601 cacaaccggc gttgttctgt gtcgaataca gcctagcgcg caccttgatt gatctgggga
     6661 ttacgccgga cagtatgatc gggcacagtc tgggcgagta tgttgcggcc tgtattgctg
     6721 gcgtatttac tcttgaggat gcgctgcacg tcattgaggc gcgcggacgt ttgatgcagt
     6781 ccatgcgtcc cggtagcatg atggcggtct accttagtcg cgaacagttg accccatggc
     6841 tagctgcaga acggggtatt gaactggcag ctaataatag cgcgcatttt tgcgtagtcg
     6901 cgggcgagca ggcggccatt tcccgtttga gcacacgctt agtcgagggc gggatacagc
     6961 acaggcgcct gaaaacctct cacgcattcc actcggccat gatgacgccg atgctgcacg
     7021 attttgcaca gttgctgggg caaatcccga tgcacgcgcc gcacaagcgc tttatatcta
     7081 atgtaagcgg tacatggatt actgaggagc aagctacctc gccggattac tgggtgcagc
     7141 aagtgcgcaa cgcggtgctg tttagcgaag gtgcggcgca actgttggta caacccacgc
     7201 tgtttatcga atgtgggccg ggtaatacgc tctctacctt tattcaagga cataaccaat
     7261 acagcgatca gccgaccctg ttgacgctac gcaaagccaa cgcggcgatc gatgatgagc
     7321 acatgttgca tcgtacgctg gcggcgctgt gggtcagggg ggagaatatt gattggagac
     7381 gctttaacca gacggcactc ggcaagcaca ttccattgcc ggattacccc ttcgaacaga
     7441 cttattacta tcgctatggt gctgcacttt ccggttatcg ccagtatcca aatcctctgc
     7501 gccgtccgca agatgagtgg ctccagcgtg tgctgtggcg gatgcacgac acatccttgc
     7561 gggaggcgtt ctatgcgccg ggcgaattga tcatcattat tagtgctgac ggcgacaagt
     7621 tacagcagac gctgatgagt agtggtgtcg acagcatcac aatgccgctg ccgatatcgt
     7681 cagaggacga cgtgtgggat aatgaccgta ttctgacgca tttccacgac atctgcgcac
     7741 ttttagcaca caaaacctac cgacagttac attgcttgta tgctcccggt gcggaggcag
     7801 gatcatcgtt gacacagtcg ctctcgggac tttatcgtgt tgctcgctgg tgtatgcaca
     7861 gcaccacgcc gctggcgtcc ttaacagtat tgacccatgg tgcgtttcgc gtacaggaag
     7921 aggataatcc cgaaccgacg ctggcagcgt tgtcgggcgc agtaaacgtg tttgcccaag
     7981 agctgcaccc gaccgaagta cgattgatcg atatcgatgc gcagagcagt gatgagaatc
     8041 tgaacttgct aacccagcgt ctggccccga aacaagaaac ggtaatggcg ctcaggcagg
     8101 ggatgcttta cctgcgacgc tttatcccga cacggctgtt ggcgcactta ccccctcaaa
     8161 cagggtgtat accgggcaac gtactgtgga ttatcggcgg tgaaaagggg attggccgca
     8221 tgatcggcga agcgcttgct cagcgtgagg gagtccgtgt ggtactgagc agccgcacag
     8281 gttatcacca tgaagcggtg cagcaggacg cattagacgt tatccactgc gacgtgacgc
     8341 aggcggaggc ggttagagct tgtctggcaa ctctgctcga acgctatgga cggttggatg
     8401 gcgtgatttt tgctgccgac gctaccacca cattgacact gcatcaattg agcgaatctg
     8461 cgctacgcga cacgctaacg gtgaaagaac ggggtactgc taatgtgctg catgcgttag
     8521 cgcaacggaa tctgctggat gagcgtctgc tactgctgtt ctgtaactcg ttggctgccg
     8581 tgaatgcgga gatcggccag acaggctatg ctaccgccag cgcctatttg gatgcactgg
     8641 cacagcaact gcgtacccgc tacaaggtga atgcgctcag tatcgggttg gatgcattgc
     8701 gtgagcaggg catgttgttg gatgctataa acggcagtga atacgatgtc ttgcgtggac
     8761 tgcgcccatt gatgacggga acgttgctac aggcttataa acaacaaggg gctgacacca
     8821 gctactacgc gcgattatcc cccgagtccg attggctgct ggatgagcac cggatatccg
     8881 gtatcgccac gctgccggga accggctatc tggcgctggc gtatgaagct ctgcgccatt
     8941 actttgtgca agaccaaatc tgcattgatg aattggtctt tttggcaccg ttgactgtga
     9001 tggacaactg cagtgttgac gtttttgttg acatttcacc taacgggcaa ggagttagtg
     9061 tcgaggtgaa atcaatgacg gagcgcttta gtggcacgtt aacaacccat gccagaggca
     9121 gggcgacgcg tctgatggta gacgataatg ttgtgtgcga tctcacgggg ctgatgcgcg
     9181 agatgcacac tatcactcct ccaacaaagg aattatcgag cacgcacttc cactatgggc
     9241 cgcgctggca cagcgtacaa caactgtatg gcaataccgc ccagactcag gttttcgcaa
     9301 cgctggccct gcccaccgtt gccgctaatg atacgatcgc actgcaccct gcgctgttgg
     9361 acatcgccag cagtgttgtc gaacaactgc ctggttttca tactgattcg gtacctttcc
     9421 tttatcagga tttacgcctg taccgcccgt tgccgaacac cttacatgtg gcgctgactg
     9481 tcaatcggca cgatgaggag ggtgacagct acgctttcac gctctacgac atggcaggcg
     9541 agatggttgc ccgctgtgcg gcaatggtga agcgcaaggt acagctccac atacaagatg
     9601 tcgatgacga cacgcgactg cgcgtgccca gtgccgataa ctaccaactg cggctggccg
     9661 ctgaggggga gggggcagga aagctagcgt tgtgccctac gccgcgctta gcgctggggg
     9721 attcacaagt agagattgag gtactggcca ccggactgaa ctttaaggat gtgctgttca
     9781 ccacgggatt gctccggcag cagccgggtg aggctccgct gcaattggga ctggagtgcg
     9841 ccggacgcat tactcgcgtg ggtaaaaatg tcactgaatt tgccccggga gaggatgtta
     9901 tggcggtgct aaacggtggt tttgtccagt acgcacgggt agaaagcgat tgcgtagtga
     9961 gaaaaccagc ccattgtcgc atcgaacagg cggctgcgct gcctatcgca tacctcaccg
    10021 cctattacgc actggtggtg cgcgctaatt tgcaacccgg agaacgagta ttgatccaca
    10081 gtgcggcggg gggcgttggc ttggctgcgc tacatattgc caaacgctgc ggagcacaga
    10141 ttttcgccac agcaggtagc gagcagaaac gcgattactt gctttcgcta ggcgtacatg
    10201 ccgtagctga ttcacacgac gaacagttcg ctgccactct gctgaccgca tcggacggac
    10261 aggggatgga tgtgatcctt aactccctca caggccgtct gcttgacgct agcctcgcgc
    10321 tgctggcacc gctgggccgt tttcttgagc tgggcagcaa ggacattgtg gaagacaaag
    10381 cgctaccgat gcgtttcttc gcccaaggcg gcacctttat tccgattaat tttcacgcgg
    10441 cacatggtgc gtttagtcgc tacctgcaac agattgtcgc ttggatagat gataacacgc
    10501 taccgctcct tccatgcaaa tccgtaccat tgcccgaggt tgcacgcgcc ttcgccaccc
    10561 tgaccacgcc gcagcatatt ggcaaggtgg tggtaacgca tcgcactgcg gcaggcatgg
    10621 accggctgaa cgccatgata gcagaaaggc gcctcggcgg ctatgcgctc agcatgagca
    10681 atgccgaggt gatgcgccaa ttgtggccga tactgaacac tcgcagtccg tgggcgcaac
    10741 tgctgctctc acctcgggcg atcgatagat tagcgcgggg caaccgggtg gatcgcggtg
    10801 taccgtctgc cgctaacgat acgattactc agcagacagt gaaaaagcgg ccccgtccgg
    10861 aaattggcgt gccttacagc cccgcgacac gtgaagtgga acgcgtgctc tgccaaatcc
    10921 tggaagagta tctgggtctg gatagagtag gcattgacga caactatgcc gaattagggg
    10981 caacctcact cgatatggtg caactgagtg ggcaaatggc gcgtcactat ccgcaagtga
    11041 gcgtggtgtc gctgtacaac cacgccaccg tgcgccagct ggcgacgttt tgccagcccc
    11101 cggagggcga gtcaaatgcg ccatcaccac agcctgcagt acagacgaat acgcgcgcga
    11161 atcagatagc aaaacgtgct ttgcaaattg cgaaaaatac tgcgcgcagt cacacgtctt
    11221 tgcattgact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
    11281 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
    11341 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
    11401 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    11461 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    11521 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    11581 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11641 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11701 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11761 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11821 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
    11881 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    11941 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
    12001 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
    12061 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
    12121 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
    12181 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
    12241 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
    12301 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
    12361 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
    12421 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
    12481 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
    12541 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
    12601 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
    12661 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
    12721 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
    12781 actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt
    12841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    13021 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    13081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    13141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    13201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    13261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    13321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    13381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    13441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    13501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    13561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    13621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
    13681 ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga
    13741 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
    13801 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    13861 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    13921 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
    13981 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
    14041 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
    14101 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
    14161 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
    14221 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
    14281 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
    14341 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
    14401 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
    14461 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
    14521 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata
    14581 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
    14641 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
    14701 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
    14761 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
    14821 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
    14881 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    14941 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgt
//
