LOCUS       sequence-227347-        5501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2G_2
FEATURES             Location/Qualifiers
     source          1..5501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(144..161)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(144..160)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..175)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             727..1260
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      1363..1951
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1852..1871
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5428..5449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5464..5469,5470..5487,5488..5494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattcgagct
       61 cggtacaccg gtctcgagga cgtcgctagc ggatcccggg gtaccgatcc tctagagtcg
      121 acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      181 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      241 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      301 cgcggccgcc ctaggttaat acccgagctt caaggaagat ttcctattaa ggttgaactt
      361 aagagcttaa gcatagacga tttgaaaaaa attttaaaac aaacaaaaaa ttctttaata
      421 aaacaatatg ttgcgatgtt taaggtttat gatttagatt taaagtttag tgaggaagct
      481 atagatagaa ttgcagagct tacttttaat atgaatcttg agagtgaaaa tcttggtgcc
      541 agaagacttc acggtgttat ggaaatagtg cttgcagatc ttttttttga agtgcctggc
      601 agtaagttga aaaaatttga aataaacttg gactatgtta ataaaaaaat acaaattaac
      661 gaacaaaaag atttgaatta ttatataatt tagttaaaag caattttaaa tgagggaggt
      721 ttccatatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
      781 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
      841 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
      901 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
      961 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
     1021 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
     1081 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     1141 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     1201 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     1261 caattcgttc aagccgagat cggcttacgc gtccaaaatc ccttaacgtg agttttcgtt
     1321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1501 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcactagatc cccggcaagt
     2041 tcatccttag ggttttcata tctcttaaaa agtcaatttc tgcttgatta atttcttgtg
     2101 gattattgtt tttgcggttt ttcatttttt atctccttga gtattgaatt ttttcttata
     2161 tataaaatat agcaaaaact atttttgcca acttttttac aaaaaatttt ttattaaaac
     2221 acttagggct ttgctaaatt ctcttttaaa agaacttagt aaagccctat ggatttaaga
     2281 actgattata cttacgtagt aaaaatacta cagatattaa tagtatagac catatcaaaa
     2341 tcataatcaa tttatattta tctaaaattg tggtccatac aaaatacaaa aaaattaggt
     2401 ttttctaaat tctattttaa aagaacttag taacacctaa tctctaaatt acttacaaaa
     2461 ttctaaaatt agatttttag ttcttcatat ttctttaaaa gttttttaag aaaatctttt
     2521 tgattttcaa aaatttcatg catcataaaa ctagtaaatt tggatttttt tttgtaaaaa
     2581 tcataacttt cttgggtttt aagttgaaat cttaatggtt ttattggatt ttgcctagat
     2641 ttttttaacg ccggactttc tttatctttt ataaaattat aagaattttt aataccattt
     2701 tctattaaat aatcttcttt tataagcccc tcttgcagtg cagttgctaa tcttaaatac
     2761 ttatacgctt gagtttttgc tacatcaaag tcttttataa aatcaagaaa agttttataa
     2821 ccatcaaatt tataatattg attatctttt atttctttta aaattttaat attttccacc
     2881 ctgtggaata tttctttttt gaaattgtca tttaactttt cctttagtgt cttgtaataa
     2941 gaaattttac tttcattaat cattaaattt tgttcaacat aagtatctat cttttttgtt
     3001 aaaactttaa caacacgttt tttttccata aaaattttcc tttatttttg caacattttc
     3061 cttaattgat ataattgtta ctattttaga ttttttaata tccacttaag aattaatccc
     3121 tcactagaat attttttatt atagaattca tatacatgtt tttctaaatc tataaattta
     3181 tcaacaattg ctttattata gggtgtatct atcttttctt ttcttaataa tcgtctcata
     3241 ttctttaaat aacaacaaac actgccggtt ttaaacctaa attctatgta atatattttt
     3301 gaaaaagtat atacattttc aaccccgttt atttcatact ttacaaaaac attatttatt
     3361 ggttttctat aaccatagta aatgcctaaa aatttgtccc cgtctttcat aggatataga
     3421 taaagttttt gaatctctga tttatctaat aatttttcaa aaaaaattat acaccgcttt
     3481 tgtttgttac taactactct atgcaatgcc tttaatattt ttgtgtgata tatattttta
     3541 tcaccttttt tttctttcag caggaagata ctttcttttg gaaatttaat tttttgttca
     3601 tttattcttg cagccttaag tccgattttc atatttaatc cttatatagc ctttctattt
     3661 aattctttta aactcaataa acttttttgg tttttcatta gttctaataa ttcgcaataa
     3721 tatgtgcaaa acactttatt gtattctatt ttcttttggt tattcaaata ttcttttata
     3781 attggcttta gaagttcaat atttacttct ttttttagtt gttcaataag gatattgaaa
     3841 atgtttgttt ttaaatcttg ataattttgt tgagaatttt ctttttttct ttcaattgac
     3901 ttctctaatt tacgctttat attgtttaaa tcgctatatt tatgactttc aataataaaa
     3961 tgaggcttaa atttgtaatt ttcgtatatt ttttggaaac ttgtttctaa ttgtttagaa
     4021 ttatattcat ttttttcaaa ttctttttga gtgttgcata gaatttcctt taatttttct
     4081 tgtttttctt tcatacaaga tttagtaaaa tgtatatttt tattttttag taatttaatt
     4141 tctattattt ttatgatttt aagtattttg attatattat ctttatcaat atttaaattg
     4201 aataaaattg taagaaaaat tgaaagaatt tttttacata aaaagttaca tttattgaag
     4261 taatttctta cttgatactt ttctatttca ttaatctttc tttcttcttt tatattattt
     4321 ttattattta aacactcctt taaatctaca ctcccatttt tagtaaatct atctttaagg
     4381 ccccttgaaa ctctattttg aaatcttaaa tctttttttt cttgaaagta tttatagatt
     4441 ttttggtaac attctttttt tgaaaaatta agttgatagt atatttcggt ccccaaattt
     4501 acccctaagt gtttgtgata attagttgtg actttaaatt ctttttctaa tttgtaaagg
     4561 taattttgca gtgtttttag tttaacgggt gtttgactat ttcttttaag gttttcatta
     4621 aagtaataga gtatgttttt ttgtgtgtat ttcttatatg ttttatttat gtacgctagt
     4681 gttgagataa ggactactaa tttatactgt aatttattgt agcaagttgt ggttttactg
     4741 gttttgttta tattagataa gttttccatt tatagctcct tgaataccgg attttttatc
     4801 tataaagtat atatatcaca attttgattt aaaagtaaat tcttttttta aaaatacaaa
     4861 aattttttat aaattatata gaccatgata ttgtattaat actaattgat tttaatttaa
     4921 ggttgaacta aaataaacat agttttgtag aaaataattt ttcattattt tgctacttga
     4981 tattggatcg taaaaatatt agggctttac aaagttcttt taaaagagaa tttagtaaag
     5041 ccctaagtct tttaataaaa aattttttgt aaaaaagttg gcaaaaatag tttttgctat
     5101 atatttatat ataagaaaaa attcaatact caaggagata aaaaatggaa aacttaaaca
     5161 ataatccaca aaaaattaat caaataaata agcaaactaa ttttattggt tttgaaaatt
     5221 ttatagaact tgataatcaa gttaatcaaa ctaaagaaaa aagtaaatta agtaagatag
     5281 gtaagaaatt acccggaata agcagtcaag tctagtgcgg ccgcgcccaa tacgcaaacc
     5341 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5401 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5461 ggctttacac tttatgcttc cggctcgtat gttgtgtgga a
//
