LOCUS       sequence-227349-        8707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ClbH-C-A2-PCP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus 
            EP
  TITLE     Colibactin assembly line enzymes use S-adenosylmethionine to build a
            cyclopropane ring.
  JOURNAL   Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 
            10.1038/nchembio.2448. Epub 2017 Aug 7.
  PUBMED    28805802
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2448"; journalName: "Nat Chem Biol"; date: 
            "2017-10"; volume: "13"; issue: "10"; pages: "1063-1065"
COMMENT     Sequence Label: pET28a-clbH-C-A2-PCP
FEATURES             Location/Qualifiers
     source          1..8707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..73
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     157..176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        157..175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    176..200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             215..237
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             256..273
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             283..300
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             304..336
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             3728..3745
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3798..3816)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3812..3859
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3896..4351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3983..4002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4193..4214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4443..5258)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4443..5258)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5166..5185
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5250..5269
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5380..5968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5869..5888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6122..6139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6154..6293
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6219..6238)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6379..6401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6395..6586)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7395..8477)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8439..8458
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8478..8555)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
       61 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
      121 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
      181 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
      241 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
      301 catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccgaatt cctgccgaac
      361 cgtttgttgg caatgtcaga gttaccactc tccgacaatg gcaagtgctg tcgtaaaacg
      421 ttgctcgact tggcgcgggc gtactcagcc agtcgcgtcg atttacgtga aactcccgcc
      481 gtgcgctacc taccgttgtc atcggctcaa tcgtcgatgt ggtttatgca acaattggcg
      541 ccgcatactg cactatacaa taaccctacc gccttgctgc ttgaaggaga actggatcgc
      601 acgcggatgg acggcgcgat tcgtcaattg atgagtcggc atactctgtt acgtgctatg
      661 gcggaaaccc acaatggaca accagtattg gcggtgcctc agtgcgtatc gtcgcaggcg
      721 ttgctgacca tagtgccact cccctcggtg agtgatgata acgcgctaca ggcgatgatc
      781 aaccagcgtg cggcacaccc catgccctta acgtcaggca caccgctgtg ccggtttgaa
      841 ctgttgacgc tcgatgacga tcgtagcgta ttgttgattc atctgcacca catcatcagt
      901 gatggctggt cgaaaggcgt tttgttacgc gaattgcagg ccgcatataa cggtgaatcg
      961 ctgacgccag aaccgttgtt ggagtatgcc gactacatgg agtaccaaga agagtggcgt
     1021 cagagtgacg cctatcagga cgcgatgcgt tactggcaaa ataccctggc ggggacgttg
     1081 ccgattcttg atatccccac cgatcaaccg cgtcagaagg tggcacgtta ccaaggcgcg
     1141 tttgttgcct tcgcattgtc tgccaacaca tgcgagcgag tgttggcggc ggcgcgtgcg
     1201 cagcgcgtgt cgttgtataa ctatctgctg accgccttcg tcctcctgct gcatcgtaat
     1261 gcgcgtcaac aggaatacat cgtcggtatg ccgattgctg cgcggctgac gaaagaacag
     1321 gagcatatga tcgcgccgct ggtcaacgta ctaccgctgc gcttacctct tgacgaagcg
     1381 gcatcgtttt ccgagttagt acagacgatc agaggtattc tgtttgccgc tttcaggcac
     1441 cagcgtctcg aatttaccga catagtgcgc gcagtgaatg tggatcgcag cgccggacat
     1501 ttcccaatat atcagtgcat gttccaactc gacaatatgc ctctggcgag cccgacacta
     1561 aacggtgtca acgttacacc attattgttg gataccagtg catctcaggt ggatatttcg
     1621 ctaagtatgc agcatatcga tgggcgcatc accggcactt tcgaatacga cgctgggtta
     1681 tacagtgcgg atcgtattca gcacctggtg gcgcagtgga gagaactgct agatgaggcc
     1741 agcagccaac ccacgcagtt agtccgcgat ttgattcgtt tcacccctcg agagcacgct
     1801 tggctggcgc ggcacaacgc cactgaagtt gctctgccgc ccgtagataa cctgctggcg
     1861 ctagtgttac cgcactgcca gcagcgccct acacaggtgg ctttgcgcca cgctgacgac
     1921 gccatgacct atggcgaatt gcaacaggcc acgatgcaga tgtgtacttg gctgcgtgcg
     1981 caaggcgtaa aacgcggaga atctgtcgca ctgcaactgc ctttttgttt cgaattgatt
     2041 atcgcacaat tggcgatcct ctctttgggg gcgagctatg tgccgctgga tggtaacgcg
     2101 ccagcggccc gcaatgccct gatcttggcg caggcaacgc cgtgcatgct gctggtggcg
     2161 caaccgctgg aatcccctca tgggctgacg ataccttggg tgctagtgcc tgactggcgt
     2221 agcctgctga cagaaatacc gaacctgcca gttagtgttg cgccagatgc tttagattgc
     2281 gatgcggtgg tgatctttac ctccggtact accgggcagc ccaaaggcgt ccgactcagc
     2341 caacgtaatc tggtcaactt aaccgcatcc tttatatcca gctatcaagt gacgcatcag
     2401 gatgttctgc tacccattac ctcagtcgcg tctgctagct ttgtcgggga ggtactgccg
     2461 ctgctcgccg ctggcggtac cttagtattg gcgcaaaaag cgcagagttt ggatagtgat
     2521 gcgctcattg cgctgctggc atctcagcgg gtgactatcc tgagcaccac cccatcgctt
     2581 tctgccagcc tctctgtgct ggctcagtcg atgggatcgc tgcgcttgtt tctgtgcggt
     2641 ggtgaagcgt tagagtatga gcagatagcg ccgcttctgc cgcacatggc agtggttaac
     2701 ggttatggcc tgaccgaaag cggtatctgc tcgacctact ttcctgtcgc aaagcgtagg
     2761 gagcaagaaa cgggagcatt gcccattggc cgcccgattc agaacaccca agcttatgtg
     2821 gtggatgcat ataatcgttt ggtaccgcca ggtgcctgtg gagaactctg tttctctggt
     2881 ctgggcattt cgcccggcta ccttgatgca cgacaggatc ccgagcgctt tgtcgagttg
     2941 ccggaatacc ctggcgttcg ggtgctgaaa actggcgatc gtgctcgttg ggcaaccgac
     3001 gggatgttgt tctatctcgg cagacaagat cgccaagtgc agatccgtgg ataccgagtt
     3061 gaactgggcg acatcgaaag cctactgaaa cagcatccgg atattgctga tgcgtgggtc
     3121 gatgtgcgac gcaatgcggc ggcaacgccg ctactagtgg ccttctattg cagcgtcaac
     3181 ggcgttgcgc tggatgctca gcaattgcgc gtatggctca gcctgcggct tccgttgcat
     3241 atgctgccgt tgctctacgt gccgctgagc gccatgccgc taggtgtaaa cggcaaaatc
     3301 gatccccagt gcctgccgct ggtcgatctg cggcagttgg aagggccggg cgagtatgtc
     3361 cctccggcaa ccgaactgga acagcgtctg gcggagatct ggcagcagtt gcttggcctg
     3421 gagcgtgtcg gcaccaccac aaatttcttc gatctcggtg gacactcact tctgctagta
     3481 cagatgcagc aatacatcgg gcagcagtgc ggtcagcacg tggcgttggt tgacctgctg
     3541 cgcttcacta ccatcaaacg cttggcggaa tttctgctgg ccccggacgc agcgcaaggg
     3601 accacaggag atcagacaca actgcgtgcg gcgaagcagc gtttggcctt tggtcacacg
     3661 cgctgggcag ccacaaccga cagtcatcac tgagagctcc gtcgacaagc ttgcggccgc
     3721 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
     3781 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     3841 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
     3901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4321 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat
     4441 tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     4501 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     4561 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     4621 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     4681 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     4741 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     4801 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     4861 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     4921 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     4981 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     5041 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     5101 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     5161 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     5221 ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     5281 atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca
     5341 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5401 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5461 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5521 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5581 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5641 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5701 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5761 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5821 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5881 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5941 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6001 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6061 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6121 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     6181 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     6241 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     6301 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6361 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6421 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     6481 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     6541 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     6601 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     6661 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     6721 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     6781 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     6841 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     6901 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     6961 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     7021 tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct
     7081 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     7141 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     7201 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     7261 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     7321 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     7381 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7441 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     7501 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     7561 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     7621 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     7681 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     7741 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     7801 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     7861 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     7921 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     7981 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     8041 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     8101 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     8161 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     8221 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     8281 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     8341 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     8401 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     8461 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     8521 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     8581 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     8641 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     8701 cataccc
//
