LOCUS       sequence-227350-       11600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the PKS module of ClbB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Zha L, Wilson MR, Brotherton CA, Balskus EP
  TITLE     Characterization of Polyketide Synthase Machinery from the pks 
            Island Facilitates Isolation of a Candidate Precolibactin.
  JOURNAL   ACS Chem Biol. 2016 May 20;11(5):1287-95. doi: 
            10.1021/acschembio.6b00014. Epub 2016 Mar 1.
  PUBMED    26890481
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.6b00014"; journalName: "ACS Chem Biol"; date: 
            "2016-05-20- 20"; volume: "11"; issue: "5"; pages: "1287-95"
COMMENT     Sequence Label: pET29b-clbBpks
FEATURES             Location/Qualifiers
     source          1..11600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     900..919
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(939..1016)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1222..1241
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1329..1348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1329..1347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1348..1372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1387..1409
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7789..7806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7859..7877)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7873..7920
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7957..8412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8044..8063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8254..8275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8504..9319)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8504..9319)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9227..9246
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9311..9330
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9441..10029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9930..9949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10183..10200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10215..10354
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10280..10299)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10440..10462
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10456..10647)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(11456..11600,1..938))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
       61 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
      121 tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
      181 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
      241 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
      301 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      361 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      421 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      481 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      541 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      601 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      661 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      721 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      781 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      841 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      901 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      961 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     1021 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     1081 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     1141 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     1201 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1261 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc
     1321 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     1381 aataattttg tttaacttta agaaggagat atacatatgc cggtggcgat tgtcggtatg
     1441 gcgggacgtt tccccggtgc ggcgaatatt gcagcgttgt ggacgctggt tgtaggaggg
     1501 gaatcgggat taacactgtt cagtgatgaa gagttgcgcg cgcatggtgt gacacctgac
     1561 acgcttaaac aagcgaatta tataaaaacc aaagggattg ttgatgatca cgaatggttt
     1621 gatgcggatt tctttggtta tactcccaac gaggcggaat gtatggatcc gcaaattcgc
     1681 ttattgcatc agtgctgctg gcaaactctg gaacacgctg ggtgcgatcc tgccaccttt
     1741 actggtgcga ttggcattta tgccggactg ctgacatccc cccactggct taatgcggta
     1801 atgcaagaca ctaccgactc taccgccctg tacaaggcca gtatcctgaa tatccattcc
     1861 gtcacagcat tgattgccca tgcgcttaac ctcaccggcc ctgccgtgac gcttgacacc
     1921 gcctgctcca cttcggcagt ggctatccac caggcctgca tcgcactacg taaccgggat
     1981 tgcgatgcgg cattggcggg cggcgtttct atcgagatgc ctgcgtaccg gggctatgaa
     2041 tatcatgaag gcatgatcaa tgcgcgagat ggtgtctgtc gtcctttcga tagccaagcc
     2101 tctggcacag tcaccggtga tggcttagga atgctgctgc taaaacgtct ggacgacgcg
     2161 ctggcggatc gggactgcat ctatggtgtg atcaaaggtt cggcggtcaa taatgatggc
     2221 aataacaaaa tcggctatac cgcgccgagt gtgatcgggc aatcaacggt aatccgcacc
     2281 agtttgcgcc gtgccggttt tgatagcgac agtatcggat tggtggaagc gcacggcacc
     2341 ggtacggtgc tgggtgatcc tattgagtta cgtgcgctta atgaggtgtt tggcccaaca
     2401 cctgttccgt tttgtgtggt ttcagcgctg aaaagtaata ttggccacct caactccgct
     2461 gcgggagtgg cgggcgtcat caaaacaacg ctcgccctgc atcatcaagt gttgccacca
     2521 acagcgcact ttcgccaact caatcctgct atagatctat cgcgttctgc attgtacgtt
     2581 aaccagcaag tccaaccgtg gccgtcgacg cgtcctcgtc gtgcactggt gagctccttc
     2641 ggcatcggcg gcaccaatgc cagcatcgca ctagaagcac atcaacatga ggacgaccct
     2701 tcagcgacgg gggtacgcga cagctatctg ttgttgttct ccgctaaaac acccgctgcg
     2761 ttagagttgc gcgtggcctc cacactggaa tatgtcaagc atggagtagg ggtgcgcctg
     2821 ccggatgtgg cttatacatt gcaaactgga cgcacagcct ttgaccatcg gcgggcttat
     2881 ttggtgagtc gtgggtcgaa aatcgatctc tcctgtgcca cgatattgca agcggaaatc
     2941 ttcaatggtc agcgcacgac agcggagatc tgcttcatgt ttcctggtca aggtagccaa
     3001 tatcacggca tggccagcgc gctctatgct catcaaccca tgtttcgcca gcacatggat
     3061 cgctgctttg ctgcattcca acgctattcg acggtcgatc tcaaggcgtt gttgtttgac
     3121 gatgaggata cgcgggatat tgatcaaacg cagttcacac aaccggcgtt gttctgtgtc
     3181 gaatacagcc tagcgcgcac cttgattgat ctggggatta cgccggacag tatgatcggg
     3241 cacagtctgg gcgagtatgt tgcggcctgt attgctggcg tatttactct tgaggatgcg
     3301 ctgcacgtca ttgaggcgcg cggacgtttg atgcagtcca tgcgtcccgg tagcatgatg
     3361 gcggtctacc ttagtcgcga acagttgacc ccatggctag ctgcagaacg gggtattgaa
     3421 ctggcagcta ataatagcgc gcatttttgc gtagtcgcgg gcgagcaggc ggccatttcc
     3481 cgtttgagca cacgcttagt cgagggcggg atacagcaca ggcgcctgaa aacctctcac
     3541 gcattccact cggccatgat gacgccgatg ctgcacgatt ttgcacagtt gctggggcaa
     3601 atcccgatgc acgcgccgca caagcgcttt atatctaatg taagcggtac atggattact
     3661 gaggagcaag ctacctcgcc ggattactgg gtgcagcaag tgcgcaacgc ggtgctgttt
     3721 agcgaaggtg cggcgcaact gttggtacaa cccacgctgt ttatcgaatg tgggccgggt
     3781 aatacgctct ctacctttat tcaaggacat aaccaataca gcgatcagcc gaccctgttg
     3841 acgctacgca aagccaacgc ggcgatcgat gatgagcaca tgttgcatcg tacgctggcg
     3901 gcgctgtggg tcagggggga gaatattgat tggagacgct ttaaccagac ggcactcggc
     3961 aagcacattc cattgccgga ttaccccttc gaacagactt attactatcg ctatggtgct
     4021 gcactttccg gttatcgcca gtatccaaat cctctgcgcc gtccgcaaga tgagtggctc
     4081 cagcgtgtgc tgtggcggat gcacgacaca tccttgcggg aggcgttcta tgcgccgggc
     4141 gaattgatca tcattattag tgctgacggc gacaagttac agcagacgct gatgagtagt
     4201 ggtgtcgaca gcatcacaat gccgctgccg atatcgtcag aggacgacgt gtgggataat
     4261 gaccgtattc tgacgcattt ccacgacatc tgcgcacttt tagcacacaa aacctaccga
     4321 cagttacatt gcttgtatgc tcccggtgcg gaggcaggat catcgttgac acagtcgctc
     4381 tcgggacttt atcgtgttgc tcgctggtgt atgcacagca ccacgccgct ggcgtcctta
     4441 acagtattga cccatggtgc gtttcgcgta caggaagagg ataatcccga accgacgctg
     4501 gcagcgttgt cgggcgcagt aaacgtgttt gcccaagagc tgcacccgac cgaagtacga
     4561 ttgatcgata tcgatgcgca gagcagtgat gagaatctga acttgctaac ccagcgtctg
     4621 gccccgaaac aagaaacggt aatggcgctc aggcagggga tgctttacct gcgacgcttt
     4681 atcccgacac ggctgttggc gcacttaccc cctcaaacag ggtgtatacc gggcaacgta
     4741 ctgtggatta tcggcggtga aaaggggatt ggccgcatga tcggcgaagc gcttgctcag
     4801 cgtgagggag tccgtgtggt actgagcagc cgcacaggtt atcaccatga agcggtgcag
     4861 caggacgcat tagacgttat ccactgcgac gtgacgcagg cggaggcggt tagagcttgt
     4921 ctggcaactc tgctcgaacg ctatggacgg ttggatggcg tgatttttgc tgccgacgct
     4981 accaccacat tgacactgca tcaattgagc gaatctgcgc tacgcgacac gctaacggtg
     5041 aaagaacggg gtactgctaa tgtgctgcat gcgttagcgc aacggaatct gctggatgag
     5101 cgtctgctac tgctgttctg taactcgttg gctgccgtga atgcggagat cggccagaca
     5161 ggctatgcta ccgccagcgc ctatttggat gcactggcac agcaactgcg tacccgctac
     5221 aaggtgaatg cgctcagtat cgggttggat gcattgcgtg agcagggcat gttgttggat
     5281 gctataaacg gcagtgaata cgatgtcttg cgtggactgc gcccattgat gacgggaacg
     5341 ttgctacagg cttataaaca acaaggggct gacaccagct actacgcgcg attatccccc
     5401 gagtccgatt ggctgctgga tgagcaccgg atatccggta tcgccacgct gccgggaacc
     5461 ggctatctgg cgctggcgta tgaagctctg cgccattact ttgtgcaaga ccaaatctgc
     5521 attgatgaat tggtcttttt ggcaccgttg actgtgatgg acaactgcag tgttgacgtt
     5581 tttgttgaca tttcacctaa cgggcaagga gttagtgtcg aggtgaaatc aatgacggag
     5641 cgctttagtg gcacgttaac aacccatgcc agaggcaggg cgacgcgtct gatggtagac
     5701 gataatgttg tgtgcgatct cacggggctg atgcgcgaga tgcacactat cactcctcca
     5761 acaaaggaat tatcgagcac gcacttccac tatgggccgc gctggcacag cgtacaacaa
     5821 ctgtatggca ataccgccca gactcaggtt ttcgcaacgc tggccctgcc caccgttgcc
     5881 gctaatgata cgatcgcact gcaccctgcg ctgttggaca tcgccagcag tgttgtcgaa
     5941 caactgcctg gttttcatac tgattcggta cctttccttt atcaggattt acgcctgtac
     6001 cgcccgttgc cgaacacctt acatgtggcg ctgactgtca atcggcacga tgaggagggt
     6061 gacagctacg ctttcacgct ctacgacatg gcaggcgaga tggttgcccg ctgtgcggca
     6121 atggtgaagc gcaaggtaca gctccacata caagatgtcg atgacgacac gcgactgcgc
     6181 gtgcccagtg ccgataacta ccaactgcgg ctggccgctg agggggaggg ggcaggaaag
     6241 ctagcgttgt gccctacgcc gcgcttagcg ctgggggatt cacaagtaga gattgaggta
     6301 ctggccaccg gactgaactt taaggatgtg ctgttcacca cgggattgct ccggcagcag
     6361 ccgggtgagg ctccgctgca attgggactg gagtgcgccg gacgcattac tcgcgtgggt
     6421 aaaaatgtca ctgaatttgc cccgggagag gatgttatgg cggtgctaaa cggtggtttt
     6481 gtccagtacg cacgggtaga aagcgattgc gtagtgagaa aaccagccca ttgtcgcatc
     6541 gaacaggcgg ctgcgctgcc tatcgcatac ctcaccgcct attacgcact ggtggtgcgc
     6601 gctaatttgc aacccggaga acgagtattg atccacagtg cggcgggggg cgttggcttg
     6661 gctgcgctac atattgccaa acgctgcgga gcacagattt tcgccacagc aggtagcgag
     6721 cagaaacgcg attacttgct ttcgctaggc gtacatgccg tagctgattc acacgacgaa
     6781 cagttcgctg ccactctgct gaccgcatcg gacggacagg ggatggatgt gatccttaac
     6841 tccctcacag gccgtctgct tgacgctagc ctcgcgctgc tggcaccgct gggccgtttt
     6901 cttgagctgg gcagcaagga cattgtggaa gacaaagcgc taccgatgcg tttcttcgcc
     6961 caaggcggca cctttattcc gattaatttt cacgcggcac atggtgcgtt tagtcgctac
     7021 ctgcaacaga ttgtcgcttg gatagatgat aacacgctac cgctccttcc atgcaaatcc
     7081 gtaccattgc ccgaggttgc acgcgccttc gccaccctga ccacgccgca gcatattggc
     7141 aaggtggtgg taacgcatcg cactgcggca ggcatggacc ggctgaacgc catgatagca
     7201 gaaaggcgcc tcggcggcta tgcgctcagc atgagcaatg ccgaggtgat gcgccaattg
     7261 tggccgatac tgaacactcg cagtccgtgg gcgcaactgc tgctctcacc tcgggcgatc
     7321 gatagattag cgcggggcaa ccgggtggat cgcggtgtac cgtctgccgc taacgatacg
     7381 attactcagc agacagtgaa aaagcggccc cgtccggaaa ttggcgtgcc ttacagcccc
     7441 gcgacacgtg aagtggaacg cgtgctctgc caaatcctgg aagagtatct gggtctggat
     7501 agagtaggca ttgacgacaa ctatgccgaa ttaggggcaa cctcactcga tatggtgcaa
     7561 ctgagtgggc aaatggcgcg tcactatccg caagtgagcg tggtgtcgct gtacaaccac
     7621 gccaccgtgc gccagctggc gacgttttgc cagcccccgg agggcgagtc aaatgcgcca
     7681 tcaccacagc ctgcagtaca gacgaatacg cgcgcgaatc agatagcaaa acgtgctttg
     7741 caaattgcga aaaatactgc gcgcagtcac acgtctttgc atctcgagca ccaccaccac
     7801 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     7861 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     7921 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
     7981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     8401 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8461 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
     8521 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     8581 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     8641 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     8701 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     8761 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     8821 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     8881 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     8941 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     9001 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     9061 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     9121 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     9181 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     9241 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     9301 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     9361 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     9601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10201 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    10261 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
    10321 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
    10381 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
    10441 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
    10501 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
    10561 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
    10621 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
    10681 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
    10741 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
    10801 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
    10861 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
    10921 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
    10981 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
    11041 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
    11101 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
    11161 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
    11221 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
    11281 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
    11341 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
    11401 cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
    11461 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11521 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    11581 gcaacagctg attgcccttc
//
