LOCUS       sequence-227359-        6734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            flagellin promoter driven iRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2G_PflaB-iRFPBb
FEATURES             Location/Qualifiers
     source          1..6734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(959..976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(959..975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(968..990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1542..2075
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      2178..2766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2667..2686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6243..6264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6279..6284,6285..6302,6303..6309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6317..6333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6322..6344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6341..6357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6341..6357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atggctgaag gtagtgttgc taggcagcct gacttattaa cttgtgacga tgaacctatt
       61 catattcctg gtgctattca acctcatggt ttattattag ctttagctgc tgatatgact
      121 attgttgctg gtagtgataa tttacctgaa ttaactggtt tagctattgg tgctttaatt
      181 ggtagaagtg ctgctgatgt ttttgatagt gaaactcata atagattaac tattgcttta
      241 gctgaacctg gtgctgctgt tggtgctcct attactgttg gttttactat gagaaaagat
      301 gctggtttta ttggtagttg gcatagacat gatcaattaa tttttttaga attagaacct
      361 cctcaaagag atgttgctga acctcaagca ttttttagaa gaactaatag tgctattaga
      421 agattacaag ctgctgaaac tttagaaagt gcttgtgctg ctgctgctca agaagttaga
      481 aaaattactg gttttgatag agttatgatt tatagatttg ctagtgattt tagtggtgaa
      541 gttattgctg aagatagatg tgctgaagtt gaaagtaaat taggtttaca ttatcctgct
      601 agtactgttc ctgctcaagc tagaagatta tatactatta atcctgttag aattattcct
      661 gatattaatt atagacctgt tcctgttact cctgatttaa atcctgttac tggtagacct
      721 attgatttaa gttttgctat tttaagaagt gttagtcctg ttcatttaga atttatgaga
      781 aatattggta tgcatggtac tatgagtatt agtattttaa gaggtgaaag attatggggt
      841 ttaattgttt gtcatcatag aactccttat tatgttgatt tagatggtag acaagcatgt
      901 gaattagttg ctcaagtttt agcttggcaa attggtgtta tggaagaata agcttggcac
      961 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1021 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1081 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcgg ccgccctagg ttaatacccg
     1141 agcttcaagg aagatttcct attaaggttg aacttaagag cttaagcata gacgatttga
     1201 aaaaaatttt aaaacaaaca aaaaattctt taataaaaca atatgttgcg atgtttaagg
     1261 tttatgattt agatttaaag tttagtgagg aagctataga tagaattgca gagcttactt
     1321 ttaatatgaa tcttgagagt gaaaatcttg gtgccagaag acttcacggt gttatggaaa
     1381 tagtgcttgc agatcttttt tttgaagtgc ctggcagtaa gttgaaaaaa tttgaaataa
     1441 acttggacta tgttaataaa aaaatacaaa ttaacgaaca aaaagatttg aattattata
     1501 taatttagtt aaaagcaatt ttaaatgagg gaggtttcca tatgttacgc agcagcaacg
     1561 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca
     1621 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt
     1681 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc
     1741 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg
     1801 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct
     1861 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca
     1921 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag
     1981 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga
     2041 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct
     2101 tacgcgtcca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2821 cacatgttct ttcctgcact agatccccgg caagttcatc cttagggttt tcatatctct
     2881 taaaaagtca atttctgctt gattaatttc ttgtggatta ttgtttttgc ggtttttcat
     2941 tttttatctc cttgagtatt gaattttttc ttatatataa aatatagcaa aaactatttt
     3001 tgccaacttt tttacaaaaa attttttatt aaaacactta gggctttgct aaattctctt
     3061 ttaaaagaac ttagtaaagc cctatggatt taagaactga ttatacttac gtagtaaaaa
     3121 tactacagat attaatagta tagaccatat caaaatcata atcaatttat atttatctaa
     3181 aattgtggtc catacaaaat acaaaaaaat taggtttttc taaattctat tttaaaagaa
     3241 cttagtaaca cctaatctct aaattactta caaaattcta aaattagatt tttagttctt
     3301 catatttctt taaaagtttt ttaagaaaat ctttttgatt ttcaaaaatt tcatgcatca
     3361 taaaactagt aaatttggat ttttttttgt aaaaatcata actttcttgg gttttaagtt
     3421 gaaatcttaa tggttttatt ggattttgcc tagatttttt taacgccgga ctttctttat
     3481 cttttataaa attataagaa tttttaatac cattttctat taaataatct tcttttataa
     3541 gcccctcttg cagtgcagtt gctaatctta aatacttata cgcttgagtt tttgctacat
     3601 caaagtcttt tataaaatca agaaaagttt tataaccatc aaatttataa tattgattat
     3661 cttttatttc ttttaaaatt ttaatatttt ccaccctgtg gaatatttct tttttgaaat
     3721 tgtcatttaa cttttccttt agtgtcttgt aataagaaat tttactttca ttaatcatta
     3781 aattttgttc aacataagta tctatctttt ttgttaaaac tttaacaaca cgtttttttt
     3841 ccataaaaat tttcctttat ttttgcaaca ttttccttaa ttgatataat tgttactatt
     3901 ttagattttt taatatccac ttaagaatta atccctcact agaatatttt ttattataga
     3961 attcatatac atgtttttct aaatctataa atttatcaac aattgcttta ttatagggtg
     4021 tatctatctt ttcttttctt aataatcgtc tcatattctt taaataacaa caaacactgc
     4081 cggttttaaa cctaaattct atgtaatata tttttgaaaa agtatataca ttttcaaccc
     4141 cgtttatttc atactttaca aaaacattat ttattggttt tctataacca tagtaaatgc
     4201 ctaaaaattt gtccccgtct ttcataggat atagataaag tttttgaatc tctgatttat
     4261 ctaataattt ttcaaaaaaa attatacacc gcttttgttt gttactaact actctatgca
     4321 atgcctttaa tatttttgtg tgatatatat ttttatcacc ttttttttct ttcagcagga
     4381 agatactttc ttttggaaat ttaatttttt gttcatttat tcttgcagcc ttaagtccga
     4441 ttttcatatt taatccttat atagcctttc tatttaattc ttttaaactc aataaacttt
     4501 tttggttttt cattagttct aataattcgc aataatatgt gcaaaacact ttattgtatt
     4561 ctattttctt ttggttattc aaatattctt ttataattgg ctttagaagt tcaatattta
     4621 cttctttttt tagttgttca ataaggatat tgaaaatgtt tgtttttaaa tcttgataat
     4681 tttgttgaga attttctttt tttctttcaa ttgacttctc taatttacgc tttatattgt
     4741 ttaaatcgct atatttatga ctttcaataa taaaatgagg cttaaatttg taattttcgt
     4801 atattttttg gaaacttgtt tctaattgtt tagaattata ttcatttttt tcaaattctt
     4861 tttgagtgtt gcatagaatt tcctttaatt tttcttgttt ttctttcata caagatttag
     4921 taaaatgtat atttttattt tttagtaatt taatttctat tatttttatg attttaagta
     4981 ttttgattat attatcttta tcaatattta aattgaataa aattgtaaga aaaattgaaa
     5041 gaattttttt acataaaaag ttacatttat tgaagtaatt tcttacttga tacttttcta
     5101 tttcattaat ctttctttct tcttttatat tatttttatt atttaaacac tcctttaaat
     5161 ctacactccc atttttagta aatctatctt taaggcccct tgaaactcta ttttgaaatc
     5221 ttaaatcttt tttttcttga aagtatttat agattttttg gtaacattct ttttttgaaa
     5281 aattaagttg atagtatatt tcggtcccca aatttacccc taagtgtttg tgataattag
     5341 ttgtgacttt aaattctttt tctaatttgt aaaggtaatt ttgcagtgtt tttagtttaa
     5401 cgggtgtttg actatttctt ttaaggtttt cattaaagta atagagtatg tttttttgtg
     5461 tgtatttctt atatgtttta tttatgtacg ctagtgttga gataaggact actaatttat
     5521 actgtaattt attgtagcaa gttgtggttt tactggtttt gtttatatta gataagtttt
     5581 ccatttatag ctccttgaat accggatttt ttatctataa agtatatata tcacaatttt
     5641 gatttaaaag taaattcttt ttttaaaaat acaaaaattt tttataaatt atatagacca
     5701 tgatattgta ttaatactaa ttgattttaa tttaaggttg aactaaaata aacatagttt
     5761 tgtagaaaat aatttttcat tattttgcta cttgatattg gatcgtaaaa atattagggc
     5821 tttacaaagt tcttttaaaa gagaatttag taaagcccta agtcttttaa taaaaaattt
     5881 tttgtaaaaa agttggcaaa aatagttttt gctatatatt tatatataag aaaaaattca
     5941 atactcaagg agataaaaaa tggaaaactt aaacaataat ccacaaaaaa ttaatcaaat
     6001 aaataagcaa actaatttta ttggttttga aaattttata gaacttgata atcaagttaa
     6061 tcaaactaaa gaaaaaagta aattaagtaa gataggtaag aaattacccg gaataagcag
     6121 tcaagtctag tgcggccgcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6181 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6241 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6301 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
     6361 attacgaatt cgagctctgt ctgtcgcctc ttgtggcttc cggaggagtt atttataata
     6421 aaataaagaa ttacgacttg aaaattttta atcaaaaaaa taaagttgaa gattataaaa
     6481 agaagtacga agatagagag agaaaagtgg aaggtgaact taataccttg gattttaccg
     6541 ttaagcgcat gaaagatcaa gaaaatacat taaaggcttt tgattttaat caaagaaata
     6601 aataataata attattttta atgctattgc tatttgcgtt tctttttttt taatttttgt
     6661 gctattcttt ttaacaggca aaaggatttg ccaaagtcag aaatttaaat tttatcatgg
     6721 aggaatgagg atcc
//
