LOCUS       sequence-227363-        5843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2_2
FEATURES             Location/Qualifiers
     source          1..5843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..90)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(73..89)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..104)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             656..1465
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(723..742)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1634..2222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2123..2142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5699..5720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5735..5740,5741..5758,5759..5765)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5773..5789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5778..5800
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5797..5813
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5797..5813
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctcgaggac gtcgctagcg gatcccgggg taccgatcct ctagagtcga cctgcaggca
       61 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      121 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      181 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcggccgccc
      241 taggttaata cccgagcttc aaggaagatt tcctattaag gttgaactta agagcttaag
      301 catagacgat ttgaaaaaaa ttttaaaaca aacaaaaaat tctttaataa aacaatatgt
      361 tgcgatgttt aaggtttatg atttagattt aaagtttagt gaggaagcta tagatagaat
      421 tgcagagctt acttttaata tgaatcttga gagtgaaaat cttggtgcca gaagacttca
      481 cggtgttatg gaaatagtgc ttgcagatct tttttttgaa gtgcctggca gtaagttgaa
      541 aaaatttgaa ataaacttgg actatgttaa taaaaaaata caaattaacg aacaaaaaga
      601 tttgaattat tatataattt agttaaaagc aattttaaat gagggaggtt tccatatggg
      661 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
      721 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      781 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      841 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      901 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
      961 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1021 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1081 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     1141 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     1201 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1261 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     1321 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     1381 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1441 tttgatgctc gatgagtttt tctaatcaga ctcgagtggc cgaggagcag gactgacacg
     1501 tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact cttttttcga
     1741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2281 tgttctttcc tgcactagat ccccggcaag ttcatcctta gggttttcat atctcttaaa
     2341 aagtcaattt ctgcttgatt aatttcttgt ggattattgt ttttgcggtt tttcattttt
     2401 tatctccttg agtattgaat tttttcttat atataaatat atagcaaaaa ctatttttgc
     2461 caactttttt acaaaaaatt ttttattaaa acacttaggg ctttgctaaa ttctctttta
     2521 aaagaactta gtaaagccct atggatttaa gaactgatta tacttacgta gtaaaatact
     2581 acagatatta atagtataga ccatatcaaa atcataatca atttatattt atctaaaatt
     2641 gtagtccata caaaatacaa aaaaattagg tttttctaaa ttctatttta aaagaactta
     2701 gtaacaccta atctctaaat tacttacaaa attctaaaat tagattttta gttcttcata
     2761 tttctttaaa agttttttaa gaaaatcttt ttgattttca aaaatttcat gcatcataaa
     2821 actagtaaat ttggattttt ttttgtaaaa atcataactt tcttgggttt taagttgaaa
     2881 tcttaatggt tttattggat tttgcctaga tttttttaac gccggacttt ctttatcttt
     2941 tataaaatta taagaatttt taataccatt ttctattaaa taatcttctt ttataagccc
     3001 ctcttgcagt gcagttgcta atcttaaata cttatacgct tgagtttttg ctacatcaaa
     3061 gtcttttata aaatcaagaa aagttttata accatcaaat ttataatatt gattatcttt
     3121 tatttctttt aaaattttaa tattttccac cctgtggaat atttcttttt tgaaattgtc
     3181 atttaacttt tcctttagtg tcttgtaata agaaatttta ctttcattaa tcattaaatt
     3241 ttgttcaaca taagtatcta tcttttttgt taaaacttta acaacacgtt ttttttccat
     3301 aaaaattttc ctttattttt gcaacatttt ccttaattga tataattgtt actattttag
     3361 attttttaat atccacttaa gaattaatcc ctcactagaa tattttttat tatagaattc
     3421 atatacatgt ttttctaaat ctataaattt atcaacaatt gctttattat agggtgtatc
     3481 tatcttttct tttcttaata atcgtctcgt attctttaaa taacaacaaa cactgccggt
     3541 tttaaaccta aattctatgt aatatatttt tgaaaaagta tatacatttt caaccccgtt
     3601 tatttcatac tttacaaaaa cattatttat tggttttcta taaccatagt aaatgcctaa
     3661 aaatttgtcc ccgtctttca taggatatag ataaagtttt tgaatctctg atttatctaa
     3721 taatttttca aaaaaaatta tacaccgctt ttgtttgtta ctaactactc tatgcaatgc
     3781 ctttaatatt tttgtgtgat atatattttt atcacctttt ttttctttca gcaggaagat
     3841 actttctttt ggaaatttaa ttttttgttc atttattctt gcagccttaa gtccgatttt
     3901 catatttaat ccttatatag cctttctatt taattctttt aaactcaata aacttttttg
     3961 gtttttcatt agttctaata attcgcaata atatgtgcaa aacactttat tgtattctat
     4021 tttcttttgg ttattcaaat attcttttat aattggcttt agaagttcaa tatttacttc
     4081 tttttttagt tgttcaataa ggatattgaa aatgtttgtt tttaaatctt gataattttg
     4141 ttgagaattt tctttttttc tttcaattga cttctctaat ttacgcttta tattgtttaa
     4201 atcgctatat ttatgacttt caataataaa atgaggctta aatttgtaat tttcgtatat
     4261 tttttggaaa cttgtttcta attgtttaga attatattca tttttttcaa attctttttg
     4321 agtgttgcat agaatttcct ttaatttttc ttgtttttct ttcatacaag atttagtaaa
     4381 atgtatattt ttatttttta gtaatttaat ttctattatt tttatgattt taagtatttt
     4441 gattatatta tctttatcaa tatttaaatt gaataaaatt gtaagaaaaa ttgaaagaat
     4501 ttttttacat aaaaagttac atttattgaa gtaatttctt acttgatact tttctatttc
     4561 attaatcttt ctttcttctt ttatattatt tttattattt aaacactcct ttaaatctac
     4621 actcccattt ttagtaaatc tatctttaag gccccttgaa actctatttt gaaatcttaa
     4681 atcttttttt tcttgaaagt atttatagat tttttggtaa cattcttttt ttgaaaaatt
     4741 aagttgatag tatatttcgg tccccaaatt tacccctaag tgtttgtgat aattagttgt
     4801 gactttaaat tctttttcta atttgtaaag gtaattttgc agtgttttta gtttaacggg
     4861 tgtttgacta tttcttttaa ggttttcatt aaagtaatag agtatgtttt tttgtgtgta
     4921 tttcttatat gttttattta tgtacgctag tgttgagata aggactacta atttatactg
     4981 taatttattg tagcaagttg tggttttact ggttttgttt atattagata agttttccat
     5041 ttatagctcc ttgaataccg gattttttat ctataaagta tatatatcac aattttgatt
     5101 taaaagtaaa ttcttttttt aaaaatacaa aaatttttta taaattatat agaccatgat
     5161 attgtattaa tactaattga ttttaattta aggttgaact aaaataaaca tagttttgta
     5221 gaaaataatt tttcattatt ttgctacttg atattggatc gtaaaaatat tagggcttta
     5281 caaagttctt ttaaaagaga atttagtaaa gccctaagtc ttttaataaa aaattttttg
     5341 taaaaaagtt ggcaaaaata gtttttgcta tatatttata tataagaaaa aattcaatac
     5401 tcaaggagat aaaaaatgga aaacttaaac aataatccac aaaaaattaa tcaaataaat
     5461 aagcaaacta attttattgg ttttgaaaat tttatagaac ttgataatca agttaatcaa
     5521 actaaagaaa aaagtaaatt aagtaagata ggtaagaaat tacccggaat aagcagtcaa
     5581 gtctagtgcg gccgcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5641 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5701 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5761 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     5821 cgaattcgag ctcggtacac cgg
//
