LOCUS       sequence-227364-        6623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            bb0026 promoter driven mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2_P0026-mCherryBb
FEATURES             Location/Qualifiers
     source          1..6623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(900..917)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(900..916)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(909..931)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1483..2292
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1550..1569)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2461..3049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2950..2969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6526..6547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6562..6567,6568..6585,6586..6592)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6600..6616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6605..6623,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgacatg attacgaatt cgagctctat tctccattct tttaaaatta
       61 ttatccttgt ttattatagt ttaaaataaa tttaattaca atttgaaatt tggtcgtttg
      121 actttcctta tattgacaat ctaagtataa tattaaggta atcttaggat ccaggaggtt
      181 catggttagt aaaggtgaag aagataatat ggctattatt aaagaattta tgagatttaa
      241 agttcacatg gaaggtagtg ttaatggtca tgaatttgaa attgaaggtg aaggtgaagg
      301 tagaccttat gaaggtactc aaactgctaa attaaaagtt actaaaggtg gtcctttacc
      361 ttttgcttgg gatattttaa gtcctcaatt tatgtatggt agtaaagcat acgttaaaca
      421 tcctgctgat attcctgatt atttaaaatt aagttttcct gaaggtttta aatgggaaag
      481 agttatgaat tttgaagatg gtggtgttgt tactgttact caagatagta gtttacaaga
      541 tggtgaattt atttataaag ttaaattaag aggtactaat tttcctagtg atggtcctgt
      601 tatgcaaaaa aaaactatgg gttgggaagc tagtagtgaa agaatgtatc ctgaagatgg
      661 tgctttaaaa ggtgaaatta aacaaagatt aaaattaaaa gatggtggtc attatgatgc
      721 tgaagttaaa actacttata aagctaaaaa acctgttcaa ttacctggtg cttataatgt
      781 taatattaaa ttagatatta cttcgcataa tgaagattat actattgttg aacaatatga
      841 aagagctgaa ggtagacata gtacaggtgg tatggatgaa ttatataaat aagcttggca
      901 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      961 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1021 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg gccgccctag gttaataccc
     1081 gagcttcaag gaagatttcc tattaaggtt gaacttaaga gcttaagcat agacgatttg
     1141 aaaaaaattt taaaacaaac aaaaaattct ttaataaaac aatatgttgc gatgtttaag
     1201 gtttatgatt tagatttaaa gtttagtgag gaagctatag atagaattgc agagcttact
     1261 tttaatatga atcttgagag tgaaaatctt ggtgccagaa gacttcacgg tgttatggaa
     1321 atagtgcttg cagatctttt ttttgaagtg cctggcagta agttgaaaaa atttgaaata
     1381 aacttggact atgttaataa aaaaatacaa attaacgaac aaaaagattt gaattattat
     1441 ataatttagt taaaagcaat tttaaatgag ggaggtttcc atatgggcca tattcaacgg
     1501 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1561 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1621 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1681 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1741 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1801 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1861 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1921 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1981 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2041 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2101 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2161 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2221 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2281 gagtttttct aatcagactc gagtggccga ggagcaggac tgacacgtgc taaaacttca
     2341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2521 agcggtggtt tgtttgccgg atcaagagct accaactctt ttttcgaagg taactggctt
     2581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3121 actagatccc cggcaagttc atccttaggg ttttcatatc tcttaaaaag tcaatttctg
     3181 cttgattaat ttcttgtgga ttattgtttt tgcggttttt cattttttat ctccttgagt
     3241 attgaatttt ttcttatata taaatatata gcaaaaacta tttttgccaa cttttttaca
     3301 aaaaattttt tattaaaaca cttagggctt tgctaaattc tcttttaaaa gaacttagta
     3361 aagccctatg gatttaagaa ctgattatac ttacgtagta aaatactaca gatattaata
     3421 gtatagacca tatcaaaatc ataatcaatt tatatttatc taaaattgta gtccatacaa
     3481 aatacaaaaa aattaggttt ttctaaattc tattttaaaa gaacttagta acacctaatc
     3541 tctaaattac ttacaaaatt ctaaaattag atttttagtt cttcatattt ctttaaaagt
     3601 tttttaagaa aatctttttg attttcaaaa atttcatgca tcataaaact agtaaatttg
     3661 gatttttttt tgtaaaaatc ataactttct tgggttttaa gttgaaatct taatggtttt
     3721 attggatttt gcctagattt ttttaacgcc ggactttctt tatcttttat aaaattataa
     3781 gaatttttaa taccattttc tattaaataa tcttctttta taagcccctc ttgcagtgca
     3841 gttgctaatc ttaaatactt atacgcttga gtttttgcta catcaaagtc ttttataaaa
     3901 tcaagaaaag ttttataacc atcaaattta taatattgat tatcttttat ttcttttaaa
     3961 attttaatat tttccaccct gtggaatatt tcttttttga aattgtcatt taacttttcc
     4021 tttagtgtct tgtaataaga aattttactt tcattaatca ttaaattttg ttcaacataa
     4081 gtatctatct tttttgttaa aactttaaca acacgttttt tttccataaa aattttcctt
     4141 tatttttgca acattttcct taattgatat aattgttact attttagatt ttttaatatc
     4201 cacttaagaa ttaatccctc actagaatat tttttattat agaattcata tacatgtttt
     4261 tctaaatcta taaatttatc aacaattgct ttattatagg gtgtatctat cttttctttt
     4321 cttaataatc gtctcgtatt ctttaaataa caacaaacac tgccggtttt aaacctaaat
     4381 tctatgtaat atatttttga aaaagtatat acattttcaa ccccgtttat ttcatacttt
     4441 acaaaaacat tatttattgg ttttctataa ccatagtaaa tgcctaaaaa tttgtccccg
     4501 tctttcatag gatatagata aagtttttga atctctgatt tatctaataa tttttcaaaa
     4561 aaaattatac accgcttttg tttgttacta actactctat gcaatgcctt taatattttt
     4621 gtgtgatata tatttttatc accttttttt tctttcagca ggaagatact ttcttttgga
     4681 aatttaattt tttgttcatt tattcttgca gccttaagtc cgattttcat atttaatcct
     4741 tatatagcct ttctatttaa ttcttttaaa ctcaataaac ttttttggtt tttcattagt
     4801 tctaataatt cgcaataata tgtgcaaaac actttattgt attctatttt cttttggtta
     4861 ttcaaatatt cttttataat tggctttaga agttcaatat ttacttcttt ttttagttgt
     4921 tcaataagga tattgaaaat gtttgttttt aaatcttgat aattttgttg agaattttct
     4981 ttttttcttt caattgactt ctctaattta cgctttatat tgtttaaatc gctatattta
     5041 tgactttcaa taataaaatg aggcttaaat ttgtaatttt cgtatatttt ttggaaactt
     5101 gtttctaatt gtttagaatt atattcattt ttttcaaatt ctttttgagt gttgcataga
     5161 atttccttta atttttcttg tttttctttc atacaagatt tagtaaaatg tatattttta
     5221 ttttttagta atttaatttc tattattttt atgattttaa gtattttgat tatattatct
     5281 ttatcaatat ttaaattgaa taaaattgta agaaaaattg aaagaatttt tttacataaa
     5341 aagttacatt tattgaagta atttcttact tgatactttt ctatttcatt aatctttctt
     5401 tcttctttta tattattttt attatttaaa cactccttta aatctacact cccattttta
     5461 gtaaatctat ctttaaggcc ccttgaaact ctattttgaa atcttaaatc ttttttttct
     5521 tgaaagtatt tatagatttt ttggtaacat tctttttttg aaaaattaag ttgatagtat
     5581 atttcggtcc ccaaatttac ccctaagtgt ttgtgataat tagttgtgac tttaaattct
     5641 ttttctaatt tgtaaaggta attttgcagt gtttttagtt taacgggtgt ttgactattt
     5701 cttttaaggt tttcattaaa gtaatagagt atgttttttt gtgtgtattt cttatatgtt
     5761 ttatttatgt acgctagtgt tgagataagg actactaatt tatactgtaa tttattgtag
     5821 caagttgtgg ttttactggt tttgtttata ttagataagt tttccattta tagctccttg
     5881 aataccggat tttttatcta taaagtatat atatcacaat tttgatttaa aagtaaattc
     5941 tttttttaaa aatacaaaaa ttttttataa attatataga ccatgatatt gtattaatac
     6001 taattgattt taatttaagg ttgaactaaa ataaacatag ttttgtagaa aataattttt
     6061 cattattttg ctacttgata ttggatcgta aaaatattag ggctttacaa agttctttta
     6121 aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa aaaagttggc
     6181 aaaaatagtt tttgctatat atttatatat aagaaaaaat tcaatactca aggagataaa
     6241 aaatggaaaa cttaaacaat aatccacaaa aaattaatca aataaataag caaactaatt
     6301 ttattggttt tgaaaatttt atagaacttg ataatcaagt taatcaaact aaagaaaaaa
     6361 gtaaattaag taagataggt aagaaattac ccggaataag cagtcaagtc tagtgcggcc
     6421 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6481 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6541 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6601 tgtgagcgga taacaatttc aca
//
