LOCUS       sequence-227366-        6836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            flagellin promoter driven mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2_PflaB-mCherryBb
FEATURES             Location/Qualifiers
     source          1..6836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    282..303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(318..323,324..341,342..348)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    356..372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     361..383
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     380..396
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     380..396
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1492..1509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1492..1508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1501..1523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2075..2884
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2142..2161)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3053..3641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3542..3561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa ctaattttat
       61 tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag aaaaaagtaa
      121 attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt gcggccgcgc
      181 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      241 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      301 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      361 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc gagctctgtc
      421 tgtcgcctct tgtggcttcc ggaggagtta tttataataa aataaagaat tacgacttga
      481 aaatttttaa tcaaaaaaat aaagttgaag attataaaaa gaagtacgaa gatagagaga
      541 gaaaagtgga aggtgaactt aataccttgg attttaccgt taagcgcatg aaagatcaag
      601 aaaatacatt aaaggctttt gattttaatc aaagaaataa ataataataa ttatttttaa
      661 tgctattgct atttgcgttt cttttttttt aatttttgtg ctattctttt taacaggcaa
      721 aaggatttgc caaagtcaga aatttaaatt ttatcatgga ggaatgagga tccatggtta
      781 gtaaaggtga agaagataat atggctatta ttaaagaatt tatgagattt aaagttcaca
      841 tggaaggtag tgttaatggt catgaatttg aaattgaagg tgaaggtgaa ggtagacctt
      901 atgaaggtac tcaaactgct aaattaaaag ttactaaagg tggtccttta ccttttgctt
      961 gggatatttt aagtcctcaa tttatgtatg gtagtaaagc atacgttaaa catcctgctg
     1021 atattcctga ttatttaaaa ttaagttttc ctgaaggttt taaatgggaa agagttatga
     1081 attttgaaga tggtggtgtt gttactgtta ctcaagatag tagtttacaa gatggtgaat
     1141 ttatttataa agttaaatta agaggtacta attttcctag tgatggtcct gttatgcaaa
     1201 aaaaaactat gggttgggaa gctagtagtg aaagaatgta tcctgaagat ggtgctttaa
     1261 aaggtgaaat taaacaaaga ttaaaattaa aagatggtgg tcattatgat gctgaagtta
     1321 aaactactta taaagctaaa aaacctgttc aattacctgg tgcttataat gttaatatta
     1381 aattagatat tacttcgcat aatgaagatt atactattgt tgaacaatat gaaagagctg
     1441 aaggtagaca tagtacaggt ggtatggatg aattatataa ataagcttgg cactggccgt
     1501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1621 acagttgcgc agcctgaatg gcgaatggcg cggccgccct aggttaatac ccgagcttca
     1681 aggaagattt cctattaagg ttgaacttaa gagcttaagc atagacgatt tgaaaaaaat
     1741 tttaaaacaa acaaaaaatt ctttaataaa acaatatgtt gcgatgttta aggtttatga
     1801 tttagattta aagtttagtg aggaagctat agatagaatt gcagagctta cttttaatat
     1861 gaatcttgag agtgaaaatc ttggtgccag aagacttcac ggtgttatgg aaatagtgct
     1921 tgcagatctt ttttttgaag tgcctggcag taagttgaaa aaatttgaaa taaacttgga
     1981 ctatgttaat aaaaaaatac aaattaacga acaaaaagat ttgaattatt atataattta
     2041 gttaaaagca attttaaatg agggaggttt ccatatgggc catattcaac gggaaacgtc
     2101 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2161 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2221 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2281 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2341 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2401 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2461 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2521 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2581 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2641 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2701 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2761 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2821 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2881 ctaatcagac tcgagtggcc gaggagcagg actgacacgt gctaaaactt catttttaat
     2941 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3001 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3061 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3121 tttgtttgcc ggatcaagag ctaccaactc ttttttcgaa ggtaactggc ttcagcagag
     3181 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcactagatc
     3721 cccggcaagt tcatccttag ggttttcata tctcttaaaa agtcaatttc tgcttgatta
     3781 atttcttgtg gattattgtt tttgcggttt ttcatttttt atctccttga gtattgaatt
     3841 ttttcttata tataaatata tagcaaaaac tatttttgcc aactttttta caaaaaattt
     3901 tttattaaaa cacttagggc tttgctaaat tctcttttaa aagaacttag taaagcccta
     3961 tggatttaag aactgattat acttacgtag taaaatacta cagatattaa tagtatagac
     4021 catatcaaaa tcataatcaa tttatattta tctaaaattg tagtccatac aaaatacaaa
     4081 aaaattaggt ttttctaaat tctattttaa aagaacttag taacacctaa tctctaaatt
     4141 acttacaaaa ttctaaaatt agatttttag ttcttcatat ttctttaaaa gttttttaag
     4201 aaaatctttt tgattttcaa aaatttcatg catcataaaa ctagtaaatt tggatttttt
     4261 tttgtaaaaa tcataacttt cttgggtttt aagttgaaat cttaatggtt ttattggatt
     4321 ttgcctagat ttttttaacg ccggactttc tttatctttt ataaaattat aagaattttt
     4381 aataccattt tctattaaat aatcttcttt tataagcccc tcttgcagtg cagttgctaa
     4441 tcttaaatac ttatacgctt gagtttttgc tacatcaaag tcttttataa aatcaagaaa
     4501 agttttataa ccatcaaatt tataatattg attatctttt atttctttta aaattttaat
     4561 attttccacc ctgtggaata tttctttttt gaaattgtca tttaactttt cctttagtgt
     4621 cttgtaataa gaaattttac tttcattaat cattaaattt tgttcaacat aagtatctat
     4681 cttttttgtt aaaactttaa caacacgttt tttttccata aaaattttcc tttatttttg
     4741 caacattttc cttaattgat ataattgtta ctattttaga ttttttaata tccacttaag
     4801 aattaatccc tcactagaat attttttatt atagaattca tatacatgtt tttctaaatc
     4861 tataaattta tcaacaattg ctttattata gggtgtatct atcttttctt ttcttaataa
     4921 tcgtctcgta ttctttaaat aacaacaaac actgccggtt ttaaacctaa attctatgta
     4981 atatattttt gaaaaagtat atacattttc aaccccgttt atttcatact ttacaaaaac
     5041 attatttatt ggttttctat aaccatagta aatgcctaaa aatttgtccc cgtctttcat
     5101 aggatataga taaagttttt gaatctctga tttatctaat aatttttcaa aaaaaattat
     5161 acaccgcttt tgtttgttac taactactct atgcaatgcc tttaatattt ttgtgtgata
     5221 tatattttta tcaccttttt tttctttcag caggaagata ctttcttttg gaaatttaat
     5281 tttttgttca tttattcttg cagccttaag tccgattttc atatttaatc cttatatagc
     5341 ctttctattt aattctttta aactcaataa acttttttgg tttttcatta gttctaataa
     5401 ttcgcaataa tatgtgcaaa acactttatt gtattctatt ttcttttggt tattcaaata
     5461 ttcttttata attggcttta gaagttcaat atttacttct ttttttagtt gttcaataag
     5521 gatattgaaa atgtttgttt ttaaatcttg ataattttgt tgagaatttt ctttttttct
     5581 ttcaattgac ttctctaatt tacgctttat attgtttaaa tcgctatatt tatgactttc
     5641 aataataaaa tgaggcttaa atttgtaatt ttcgtatatt ttttggaaac ttgtttctaa
     5701 ttgtttagaa ttatattcat ttttttcaaa ttctttttga gtgttgcata gaatttcctt
     5761 taatttttct tgtttttctt tcatacaaga tttagtaaaa tgtatatttt tattttttag
     5821 taatttaatt tctattattt ttatgatttt aagtattttg attatattat ctttatcaat
     5881 atttaaattg aataaaattg taagaaaaat tgaaagaatt tttttacata aaaagttaca
     5941 tttattgaag taatttctta cttgatactt ttctatttca ttaatctttc tttcttcttt
     6001 tatattattt ttattattta aacactcctt taaatctaca ctcccatttt tagtaaatct
     6061 atctttaagg ccccttgaaa ctctattttg aaatcttaaa tctttttttt cttgaaagta
     6121 tttatagatt ttttggtaac attctttttt tgaaaaatta agttgatagt atatttcggt
     6181 ccccaaattt acccctaagt gtttgtgata attagttgtg actttaaatt ctttttctaa
     6241 tttgtaaagg taattttgca gtgtttttag tttaacgggt gtttgactat ttcttttaag
     6301 gttttcatta aagtaataga gtatgttttt ttgtgtgtat ttcttatatg ttttatttat
     6361 gtacgctagt gttgagataa ggactactaa tttatactgt aatttattgt agcaagttgt
     6421 ggttttactg gttttgttta tattagataa gttttccatt tatagctcct tgaataccgg
     6481 attttttatc tataaagtat atatatcaca attttgattt aaaagtaaat tcttttttta
     6541 aaaatacaaa aattttttat aaattatata gaccatgata ttgtattaat actaattgat
     6601 tttaatttaa ggttgaacta aaataaacat agttttgtag aaaataattt ttcattattt
     6661 tgctacttga tattggatcg taaaaatatt agggctttac aaagttcttt taaaagagaa
     6721 tttagtaaag ccctaagtct tttaataaaa aattttttgt aaaaaagttg gcaaaaatag
     6781 tttttgctat atatttatat ataagaaaaa attcaatact caaggagata aaaaat
//
