LOCUS       sequence-227372-        5469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Pu J, Zinkus-Boltz J, Dickinson BC
  TITLE     Evolution of a split RNA polymerase as a versatile biosensor 
            platform.
  JOURNAL   Nat Chem Biol. 2017 Apr;13(4):432-438. doi: 10.1038/nchembio.2299. 
            Epub 2017 Feb 13.
  PUBMED    28192413
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2299"; journalName: "Nat Chem Biol"; date: 
            "2017-04"; volume: "13"; issue: "4"; pages: "432-438"
COMMENT     Sequence Label: p5-39
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             join(335..337,338..658)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     terminator      2818..2849
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2865..3524)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      complement(3677..4415)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     terminator      4505..4550
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4565..5443)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga
      301 cgcgtacaac tcaagtctga cataaatgac cgctatggga gttcaggtag agacaattag
      361 tcctggggat ggccgcacat ttccgaaacg cggccaaacc tgtgtggtac actatacagg
      421 aatgttggaa gatggcaaaa agtttgattc cagtcgtgat cgcaacaagc cttttaaatt
      481 tatgttggga aaacaggagg taattcgcgg ttgggaggaa ggtgtcgcac aaatgtccgt
      541 gggtcaacgc gccaagttga cgatctcacc tgattacgct tacggagcca ccggccatcc
      601 tggcattatt cccccacatg ccactttggt ctttgatgtt gaacttttaa agttagaaac
      661 gtcgggtgga agcggtaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
      721 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt
      781 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
      841 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat
      901 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc
      961 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct
     1021 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc
     1081 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt
     1141 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc
     1201 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct
     1261 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg
     1321 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat
     1381 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc
     1441 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa
     1501 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc
     1561 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa
     1621 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt
     1681 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct
     1741 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt
     1801 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt
     1861 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt
     1921 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa
     1981 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt
     2041 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac
     2101 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc
     2161 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga
     2221 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa
     2281 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc
     2341 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca
     2401 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca
     2461 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac
     2521 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt
     2581 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga
     2641 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca
     2701 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga
     2761 catcttagag tcggacttcg cgttcgcgta aacttaatta acggcactcc tcagccaagt
     2821 caaaagcctc cgaccggagg cttttgacta catgcccatg gcgtttacgc cccgccctgc
     2881 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     2941 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     3001 ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     3061 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     3121 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     3181 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     3241 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     3301 atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     3361 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     3421 tggttatagg tacattgagt aactgactga aatgcctcaa aatgttcttt acgatgccat
     3481 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     3541 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     3601 aagttggaac ctcttacgtg ccaagccaaa taggccgtca ctcggtcgct acgctccggg
     3661 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
     3721 gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg
     3781 ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc
     3841 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     3901 accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     3961 atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact
     4021 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     4081 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
     4141 acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     4201 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     4261 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
     4321 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc
     4381 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt
     4441 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag cccaggagga agaggacatc
     4501 cggtcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta gacttaggga
     4561 ccctttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     4621 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     4681 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     4741 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     4801 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     4861 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     4921 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     4981 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     5041 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     5101 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     5161 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     5221 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     5281 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     5341 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     5401 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccac
     5461 cattcagag
//
