LOCUS       sequence-227417-        6441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Pear WS, Miller JP, Xu L, Pui JC, Soffer B, Quackenbush RC, 
            Pendergast AM, Bronson R, Aster JC, Scott ML, Baltimore D
  TITLE     Efficient and rapid induction of a chronic myelogenous leukemia-like
            myeloproliferative disease in mice receiving P210 bcr/abl-transduced
            bone marrow.
  JOURNAL   Blood. 1998 Nov 15. 92(10):3780-92.
  PUBMED    9808572
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 1998
            Nov 15. 92(10):3780-92."
COMMENT     Sequence Label: MIGR1
FEATURES             Location/Qualifiers
     source          1..6441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    157..724
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    174..726
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(318..335)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     545..564
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(738..740,741..743,744..1457)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(783..804)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1044..1063)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1391..1412
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1559..2073
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2235..2251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2259..2265,2266..2283,2284..2289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2304..2325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2442..2459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2613..3201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2693..2712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3372..4163,4164..4232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3995..4014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4233..4337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4405..4423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4461..4483)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4583..4602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4796..4818
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4946..4965
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5165..5681
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    5745..6086
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(6153..6441,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatct ctcgaggtta acgaattccg cccccccccc ctaacgttac
      181 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
      241 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
      301 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
      361 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
      421 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
      481 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
      541 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
      601 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
      661 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
      721 ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      781 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg
      841 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      901 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      961 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1021 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1081 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1141 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1201 ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg
     1261 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1321 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1381 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1441 acgagctgta caagtaatga attaattaag aattatcaag cttatcgata ccgtcgacct
     1501 gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     1561 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     1621 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
     1681 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     1741 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc
     1801 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     1861 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     1921 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
     1981 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     2041 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
     2101 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
     2161 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
     2221 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2281 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2341 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2401 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2461 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4381 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4441 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4501 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4561 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4621 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     4681 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4741 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     4801 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
     4861 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     4921 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     4981 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
     5041 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     5101 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     5161 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     5221 aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag
     5281 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     5341 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag
     5401 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     5461 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     5521 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtattc
     5581 ccaataaagc ctcttgctgt ttgcatccga atcgtggact cgctgatcct tgggagggtc
     5641 tcctcagatt gattgactgc ccacctcggg ggtctttcat ttggaggttc caccgagatt
     5701 tggagacccc tgcccaggga ccaccgaccc ccccgccggg aggtaagctg gccagcggtc
     5761 gtttcgtgtc tgtctctgtc tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc
     5821 gtctgtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     5881 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     5941 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     6001 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     6061 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     6121 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     6181 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     6241 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     6301 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     6361 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     6421 ttgacccccc tccctgggtc a
//
