LOCUS       sequence-227421-       12450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   He Y, Wertheim JA, Xu L, Miller JP, Karnell FG, Choi JK, Ren R, Pear
            WS.
  TITLE     The coiled-coil domain and Tyr177 of bcr are required to induce a 
            murine chronic myelogenous leukemia-like disease by bcr/abl.
  JOURNAL   Blood. 2002 Apr 15;99(8):2957-68.
  PUBMED    11929787
REFERENCE   2  (bases 1 to 12450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood."; 
            date: "2002-04-15"; volume: "99(8)"; pages: "2957-68"
COMMENT     Sequence Label: P210 pcDNA3
FEATURES             Location/Qualifiers
     source          1..12450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8003..8021)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8004..8021)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8041..8058)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8047..8271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8317..8745
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8404..8423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8614..8635
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8754..8774)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8759..9088
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8939..9074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9001..9020
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9155..9949
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9209..9228)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9819..9838
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10123..10244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10160..10179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10214..10233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10293..10309)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10293..10309)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10306..10328)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10317..10333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10341..10347,10348..10365,10366..10371))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10386..10407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10524..10541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10695..11283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10775..10794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11454..12245,12246..12314))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12077..12096
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12315..12419)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttccgcgggg ccatgggggc
      961 cgcccggcgc ccggggccgg gcctggcgag gccgccgcgc cgccgctgag acgggccccg
     1021 cgcgcagccc ggcggcgcag gtaaggccgg ccgcgccatg gtggacccgg tgggcttcgc
     1081 ggaggcgtgg aaggcgcagt tcccggactc agagcccccg cgcatggagc tgcgctcagt
     1141 gggcgacatc gagcaggagc tggagcgctg caaggcctcc attcggcgcc tggagcagga
     1201 ggtgaaccag gagcgcttcc gcatgatcta cctgcagacg ttgctggcca aggaaaagaa
     1261 gagctatgac cggcagcgat ggggcttccg gcgcgcggcg caggcccccg acggcgcctc
     1321 cgagccccga gcgtccgcgt cgcgcccgca gccagcgccc gccgacggag ccgacccgcc
     1381 gcccgccgag gagcccgagg cccggcccga cggcgagggt tctccgggta aggccaggcc
     1441 cgggaccgcc cgcaggcccg gggcagccgc gtcgggggaa cgggacgacc ggggaccccc
     1501 cgccagcgtg gcggcgctca ggtccaactt cgagcggatc cgcaagggcc atggccagcc
     1561 cggggcggac gccgagaagc ccttctacgt gaacgtcgag tttcaccacg agcgcggcct
     1621 ggtgaaggtc aacgacaaag aggtgtcgga ccgcatcagc tccctgggca gccaggccat
     1681 gcagatggag cgcaaaaagt cccagcacgg cgcgggctcg agcgtggggg atgcatccag
     1741 gcccccttac cggggacgct cctcggagag cagctgcggc gtcgacggcg actacgagga
     1801 cgccgagttg aacccccgct tcctgaagga caacctgatc gacgccaatg gcggtagcag
     1861 gcccccttgg ccgcccctgg agtaccagcc ctaccagagc atctacgtcg ggggcatgat
     1921 ggaaggggag ggcaagggcc cgctcctgcg cagccagagc acctctgagc aggagaagcg
     1981 ccttacctgg ccccgcaggt cctactcccc ccggagtttt gaggattgcg gaggcggcta
     2041 taccccggac tgcagctcca atgagaacct cacctccagc gaggaggact tctcctctgg
     2101 ccagtccagc cgcgtgtccc caagccccac cacctaccgc atgttccggg acaaaagccg
     2161 ctctccctcg cagaactcgc aacagtcctt cgacagcagc agtcccccca cgccgcagtg
     2221 ccataagcgg caccggcact gcccggttgt cgtgtccgag gccaccatcg tgggcgtccg
     2281 caagaccggg cagatctggc ccaacgatgg cgagggcgcc ttccatggag acgcagatgg
     2341 ctcgttcgga acaccacctg gatacggctg cgctgcagac cgggcagagg agcagcgccg
     2401 gcaccaagat gggctgccct acattgatga ctcgccctcc tcatcgcccc acctcagcag
     2461 caagggcagg ggcagccggg atgcgctggt ctcgggagcc ctggagtcca ctaaagcgag
     2521 tgagctggac ttggaaaagg gcttggagat gagaaaatgg gtcctgtcgg gaatcctggc
     2581 tagcgaggag acttacctga gccacctgga ggcactgctg ctgcccatga agcctttgaa
     2641 agccgctgcc accacctctc agccggtgct gacgagtcag cagatcgaga ccatcttctt
     2701 caaagtgcct gagctctacg agatccacaa ggagttctat gatgggctct tcccccgcgt
     2761 gcagcagtgg agccaccagc agcgggtggg cgacctcttc cagaagctgg ccagccagct
     2821 gggtgtgtac cgggccttcg tggacaacta cggagttgcc atggaaatgg ctgagaagtg
     2881 ctgtcaggcc aatgctcagt ttgcagaaat ctccgagaac ctgagagcca gaagcaacaa
     2941 agatgccaag gatccaacga ccaagaactc tctggaaact ctgctctaca agcctgtgga
     3001 ccgtgtgacg aggagcacgc tggtcctcca tgacttgctg aagcacactc ctgccagcca
     3061 ccctgaccac cccttgctgc aggacgccct ccgcatctca cagaacttcc tgtccagcat
     3121 caatgaggag atcacacccc gacggcagtc catgacggtg aagaagggag agcaccggca
     3181 gctgctgaag gacagcttca tggtggagct ggtggagggg gcccgcaagc tgcgccacgt
     3241 cttcctgttc accgacctgc ttctctgcac caagctcaag aagcagagcg gaggcaaaac
     3301 gcagcagtat gactgcaaat ggtacattcc gctcacggat ctcagcttcc agatggtgga
     3361 tgaactggag gcagtgccca acatccccct ggtgcccgat gaggagctgg acgctttgaa
     3421 gatcaagatc tcccagatca agagtgacat ccagagagag aagagggcga acaagggcag
     3481 caaggctacg gagaggctga agaagaagct gtcggagcag gagtcactgc tgctgcttat
     3541 gtctcccagc atggccttca gggtgcacag ccgcaacggc aagagttaca cgttcctgat
     3601 ctcctctgac tatgagcgtg cagagtggag ggagaacatc cgggagcagc agaagaagtg
     3661 tttcagaagc ttctccctga catccgtgga gctgcagatg ctgaccaact cgtgtgtgaa
     3721 actccagact gtccacagca ttccgctgac catcaataag gaagatgatg agtctccggg
     3781 gctctatggg tttctgaatg tcatcgtcca ctcagccact ggatttaagc agagttcaaa
     3841 agcccttcag cggccagtag catctgactt tgagcctcag ggtctgagtg aagccgctcg
     3901 ttggaactcc aaggaaaacc ttctcgctgg acccagtgaa aatgacccca accttttcgt
     3961 tgcactgtat gattttgtgg ccagtggaga taacactcta agcataacta aaggtgaaaa
     4021 gctccgggtc ttaggctata atcacaatgg ggaatggtgt gaagcccaaa ccaaaaatgg
     4081 ccaaggctgg gtcccaagca actacatcac gccagtcaac agtctggaga aacactcctg
     4141 gtaccatggg cctgtgtccc gcaatgccgc tgagtatctg ctgagcagcg ggatcaatgg
     4201 cagcttcttg gtgcgtgaga gtgagagcag tcctggccag aggtccatct cgctgagata
     4261 cgaagggagg gtgtaccatt acaggatcaa cactgcttct gatggcaagc tctacgtctc
     4321 ctccgagagc cgcttcaaca ccctggccga gttggttcat catcattcaa cggtggccga
     4381 cgggctcatc accacgctcc attatccagc cccaaagcgc aacaagccca ctgtctatgg
     4441 tgtgtccccc aactacgaca agtgggagat ggaacgcacg gacatcacca tgaagcacaa
     4501 gctgggcggg ggccagtacg gggaggtgta cgagggcgtg tggaagaaat acagcctgac
     4561 ggtggccgtg aagaccttga aggaggacac catggaggtg gaagagttct tgaaagaagc
     4621 tgcagtcatg aaagagatca aacaccctaa cctggtgcag ctccttgggg tctgcacccg
     4681 ggagcccccg ttctatatca tcactgagtt catgacctac gggaacctcc tggactacct
     4741 gagggagtgc aaccggcagg aggtgaacgc cgtggtgctg ctgtacatgg ccactcagat
     4801 ctcgtcagcc atggagtacc tggagaagaa aaacttcatc cacagagatc ttgctgcccg
     4861 aaactgcctg gtaggggaga accacttggt gaaggtagct gattttggcc tgagcaggtt
     4921 gatgacaggg gacacctaca cagcccatgc tggagccaag ttccccatca aatggactgc
     4981 acccgagagc ctggcctaca acaagttctc catcaagtcc gacgtctggg catttggagt
     5041 attgctttgg gaaattgcta cctatggcat gtccccttac ccgggaattg acctgtccca
     5101 ggtgtatgag ctgctagaga aggactaccg catggagcgc ccagaaggct gcccagagaa
     5161 ggtctatgaa ctcatgcgag catgttggca gtggaatccc tctgaccggc cctcctttgc
     5221 tgaaatccac caagcctttg aaacaatgtt ccaggaatcc agtatctcag acgaagtgga
     5281 aaaggagctg gggaaacaag gcgtccgtgg ggctgtgagt accttgctgc aggccccaga
     5341 gctgcccacc aagacgagga cctccaggag agctgcagag cacagagaca ccactgacgt
     5401 gcctgagatg cctcactcca agggccaggg agagagcgat cctctggacc atgagcctgc
     5461 cgtgtctcca ttgctccctc gaaaagagcg aggtcccccg gagggcggcc tgaatgaaga
     5521 tgagcgcctt ctccccaaag acaaaaagac caacttgttc agcgccttga tcaagaagaa
     5581 gaagaagaca gccccaaccc ctcccaaacg cagcagctcc ttccgggaga tggacggcca
     5641 gccggagcgc agaggggccg gcgaggaaga gggccgagac atcagcaacg gggcactggc
     5701 tttcaccccc ttggacacag ctgacccagc caagtcccca aagcccagca atggggctgg
     5761 ggtccccaat ggagccctcc gggagtccgg gggctcaggc ttccggtctc cccacctgtg
     5821 gaagaagtcc agcacgctga ccagcagccg cctagccacc ggcgaggagg agggcggtgg
     5881 cagctccagc aagcgcttcc tgcgctcttg ctccgcctcc tgcgttcccc atggggccaa
     5941 ggacacggag tggaggtcag tcacgctgcc tcgggacttg cagtccacgg gaagacagtt
     6001 tgactcgtcc acatttggag ggcacaaaag tgagaagccg gctctgcctc ggaagagggc
     6061 aggggagaac aggtctgacc aggtgacccg aggcacagta acgcctcccc ccaggctggt
     6121 gaaaaagaat gaggaagctg ctgatgaggt cttcaaagac atcatggagt ccagcccggg
     6181 ctccagcccg cccaacctga ctccaaaacc cctccggcgg caggtcaccg tggcccctgc
     6241 ctcgggcctc ccccacaagg aagaagctgg aaagggcagt gccttaggga cccctgctgc
     6301 agctgagcca gtgaccccca ccagcaaagc aggctcaggt gcaccagggg gcaccagcaa
     6361 gggccccgcc gaggagtcca gagtgaggag gcacaagcac tcctctgagt cgccagggag
     6421 ggacaagggg aaattgtcca ggctcaaacc tgccccgccg cccccaccag cagcctctgc
     6481 agggaaggct ggaggaaagc cctcgcagag cccgagccag gaggcggccg gggaggcagt
     6541 cctgggcgca aagacaaaag ccacgagtct ggttgatgct gtgaacagtg acgctgccaa
     6601 gcccagccag ccgggagagg gcctcaaaaa gcccgtgctc ccggccactc caaagccaca
     6661 gtccgccaag ccgtcgggga cccccatcag cccagccccc gttccctcca cgttgccatc
     6721 agcatcctcg gccctggcag gggaccagcc gtcttccacc gccttcatcc ctctcatatc
     6781 aacccgagtg tctcttcgga aaacccgcca gcctccagag cggatcgcca gcggcgccat
     6841 caccaagggc gtggtcctgg acagcaccga ggcgctgtgc ctcgccatct ctaggaactc
     6901 cgagcagatg gccagccaca gcgcagtgct ggaggccggc aaaaacctct actcgttctg
     6961 cgtgagctat gtggattcca tccagcaaat gaggaacaag tttgccttcc gagaggccat
     7021 caacaaactg gagaataatc tccgggagct tcagatctgc ccggcgacag caggcagtgg
     7081 tccagcggcc actcaggact tcagcaagct cctcagttcg gtgaaggaaa tcagtgacat
     7141 agtgcagagg tagcagcagt caggggtcag gtgtcaggcc cgtcggagct gcctgcagca
     7201 catgcgggct cgcccatacc cgtgacagtg gctgacaagg gactagtgag tcagcacctt
     7261 ggcccaggag ctctgcgcca ggcagagctg agggccctgt ggagtccagc tctactacct
     7321 acgtttgcac cgcctgccct cccgcacctt cctcctcccc gctccgtctc tgtcctcgaa
     7381 ttttatctgt ggagttcctg ctccgtggac tgcagtcggc atgccaggac ccgccagccc
     7441 cgctcccacc tagtgcccca gactgagctc tccaggccag gtgggaacgg ctgatgtgga
     7501 ctgtcttttt catttttttc tctctggagc ccctcctccc ccggctgggc ctccttcttc
     7561 cacttctcca agaatggaag cctgaactga ggccttgtgt gtcaggccct ctgcctgcac
     7621 tccctggcct tgcccgtcgt gtgctgaaga catgtttcaa gaaccgcatt tcgggaaggg
     7681 catgcacggg catgcacacg gctggtcact ctgccctctg ctgctgcccg gggtggggtg
     7741 cactcgccat ttcctcacgt gcaggacagc tcttgatttg ggtggaaaac agggtgctaa
     7801 agccaaccag cctttgggtc ctgggcaggt gggagctgaa aaggatcgag gcatggggca
     7861 tgtcctttcc atctgtccac atccccagag cccagctctt gctctcttgt gacgtgcact
     7921 gtgaatcctg gcaagaaagc tggaattctg cagatatcca tcacactggc ggccgctcga
     7981 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     8041 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8101 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8161 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8221 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8281 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     8341 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8401 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8461 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8521 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8581 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8641 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8701 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     8761 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8821 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8881 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9001 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     9061 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     9121 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     9181 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     9241 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     9301 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     9361 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     9421 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     9481 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     9541 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     9601 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     9661 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     9721 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9781 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9841 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9901 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     9961 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
    10021 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    10081 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
    10141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
    10261 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    10321 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    10381 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    10441 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    10501 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10561 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    11041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    11101 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    11161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    11221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    11281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    11341 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    11401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    11461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    11521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    11581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    11641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    11701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11761 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    12001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    12061 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    12121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    12181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    12241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    12301 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    12361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    12421 acatttcccc gaaaagtgcc acctgacgtc
//
