LOCUS       sequence-227425-        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Offterdinger M, Bastiaens PI
  TITLE     Prolonged EGFR signaling by ERBB2-mediated sequestration at the 
            plasma membrane.
  JOURNAL   Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13.
  PUBMED    17956594
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2008-01"; volume: "9(1)"; pages: "147-55. Epub 2007 Nov 13"
COMMENT     Sequence Label: perbB2-EGFP
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4714..4716,4717..4719,4720..5430)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4714..4716,4717..4719,4720..5430)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4759..4780)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5020..5039)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5367..5388
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5477..5494)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5483..5707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5753..6181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5840..5859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6050..6071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6190..6210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6195..6524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6375..6510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6437..6456
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6591..7385
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6645..6664)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7255..7274
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7559..7680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7596..7615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7650..7669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7729..7745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7729..7745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7742..7764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7753..7769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7777..7783,7784..7801,7802..7807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7822..7843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7960..7977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8131..8719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8211..8230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8890..9681,9682..9750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9513..9532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9751..9855)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggagc tggcggcctt gtgccgctgg gggctcctcc tcgccctctt gccccccgga
      961 gccgcgagca cccaagtgtg caccggcaca gacatgaagc tgcggctccc tgccagtccc
     1021 gagacccacc tggacatgct ccgccacctc taccagggct gccaggtggt gcagggaaac
     1081 ctggaactca cctacctgcc caccaatgcc agcctgtcct tcctgcagga tatccaggag
     1141 gtgcagggct acgtgctcat cgctcacaac caagtgaggc aggtcccact gcagaggctg
     1201 cggattgtgc gaggcaccca gctctttgag gacaactatg ccctggccgt gctagacaat
     1261 ggagacccgc tgaacaatac cacccctgtc acaggggcct ccccaggagg cctgcgggag
     1321 ctgcagcttc gaagcctcac agagatcttg aaaggagggg tcttgatcca gcggaacccc
     1381 cagctctgct accaggacac gattttgtgg aaggacatct tccacaagaa caaccagctg
     1441 gctctcacac tgatagacac caaccgctct cgggcctgcc acccctgttc tccgatgtgt
     1501 aagggctccc gctgctgggg agagagttct gaggattgtc agagcctgac gcgcactgtc
     1561 tgtgccggtg gctgtgcccg ctgcaagggg ccactgccca ctgactgctg ccatgagcag
     1621 tgtgctgccg gctgcacggg ccccaagcac tctgactgcc tggcctgcct ccacttcaac
     1681 cacagtggca tctgtgagct gcactgccca gccctggtca cctacaacac agacacgttt
     1741 gagtccatgc ccaatcccga gggccggtat acattcggcg ccagctgtgt gactgcctgt
     1801 ccctacaact acctttctac ggacgtggga tcctgcaccc tcgtctgccc cctgcacaac
     1861 caagaggtga cagcagagga tggaacacag cggtgtgaga agtgcagcaa gccctgtgcc
     1921 cgagtgtgct atggtctggg catggagcac ttgcgagagg tgagggcagt taccagtgcc
     1981 aatatccagg agtttgctgg ctgcaagaag atctttggga gcctggcatt tctgccggag
     2041 agctttgatg gggacccagc ctccaacact gccccgctcc agccagagca gctccaagtg
     2101 tttgagactc tggaagagat cacaggttac ctatacatct cagcatggcc ggacagcctg
     2161 cctgacctca gcgtcttcca gaacctgcaa gtaatccggg gacgaattct gcacaatggc
     2221 gcctactcgc tgaccctgca agggctgggc atcagctggc tggggctgcg ctcactgagg
     2281 gaactgggca gtggactggc cctcatccac cataacaccc acctctgctt cgtgcacacg
     2341 gtgccctggg accagctctt tcggaacccg caccaagctc tgctccacac tgccaaccgg
     2401 ccagaggacg agtgtgtggg cgagggcctg gcctgccacc agctgtgcgc ccgagggcac
     2461 tgctggggtc cagggcccac ccagtgtgtc aactgcagcc agttccttcg gggccaggag
     2521 tgcgtggagg aatgccgagt actgcagggg ctccccaggg agtatgtgaa tgccaggcac
     2581 tgtttgccgt gccaccctga gtgtcagccc cagaatggct cagtgacctg ttttggaccg
     2641 gaggctgacc agtgtgtggc ctgtgcccac tataaggacc ctcccttctg cgtggcccgc
     2701 tgccccagcg gtgtgaaacc tgacctctcc tacatgccca tctggaagtt tccagatgag
     2761 gagggcgcat gccagccttg ccccatcaac tgcacccact cctgtgtgga cctggatgac
     2821 aagggctgcc ccgccgagca gagagccagc cctctgacgt ccatcatctc tgcggtggtt
     2881 ggcattctgc tggtcgtggt cttgggggtg gtctttggga tcctcatcaa gcgacggcag
     2941 cagaagatcc ggaagtacac gatgcggaga ctgctgcagg aaacggagct ggtggagccg
     3001 ctgacaccta gcggagcgat gcccaaccag gcgcagatgc ggatcctgaa agagacggag
     3061 ctgaggaagg tgaaggtgct tggatctggc gcttttggca cagtctacaa gggcatctgg
     3121 atccctgatg gggagaatgt gaaaattcca gtggccatca aagtgttgag ggaaaacaca
     3181 tcccccaaag ccaacaaaga aatcttagac gaagcatacg tgatggctgg tgtgggctcc
     3241 ccatatgtct cccgccttct gggcatctgc ctgacatcca cggtgcagct ggtgacacag
     3301 cttatgccct atggctgcct cttagaccat gtccgggaaa accgcggacg cctgggctcc
     3361 caggacctgc tgaactggtg tatgcagatt gccaagggga tgagctacct ggaggatgtg
     3421 cggctcgtac acagggactt ggccgctcgg aacgtgctgg tcaagagtcc caaccatgtc
     3481 aaaattacag acttcgggct ggctcggctg ctggacattg acgagacaga gtaccatgca
     3541 gatgggggca aggtgcccat caagtggatg gcgctggagt ccattctccg ccggcggttc
     3601 acccaccaga gtgatgtgtg gagttatggt gtgactgtgt gggagctgat gacttttggg
     3661 gccaaacctt acgatgggat cccagcccgg gagatccctg acctgctgga aaagggggag
     3721 cggctgcccc agccccccat ctgcaccatt gatgtctaca tgatcatggt caaatgttgg
     3781 atgattgact ctgaatgtcg gccaagattc cgggagttgg tgtctgaatt ctcccgcatg
     3841 gccagggacc cccagcgctt tgtggtcatc cagaatgagg acttgggccc agccagtccc
     3901 ttggacagca ccttctaccg ctcactgctg gaggacgatg acatggggga cctggtggat
     3961 gctgaggagt atctggtacc ccagcagggc ttcttctgtc cagaccctgc cccgggcgct
     4021 gggggcatgg tccaccacag gcaccgcagc tcatctacca ggagtggcgg tggggacctg
     4081 acactagggc tggagccctc tgaagaggag gcccccaggt ctccactggc accctccgaa
     4141 ggggctggct ccgatgtatt tgatggtgac ctgggaatgg gggcagccaa ggggctgcaa
     4201 agcctcccca cacatgaccc cagccctcta cagcggtaca gtgaggaccc cacagtaccc
     4261 ctgccctctg agactgatgg ctacgttgcc cccctgacct gcagccccca gcctgaatat
     4321 gtgaaccagc cagatgttcg gccccagccc ccttcgcccc gagagggccc tctgcctgct
     4381 gcccgacctg ctggtgccac tctggaaagg cccaagactc tctccccagg gaagaatggg
     4441 gtcgtcaaag acgtttttgc ctttgggggt gccgtggaga accccgagta cttgacaccc
     4501 cagggaggag ctgcccctca gccccaccct cctcctgcct tcagcccagc cttcgacaac
     4561 ctctattact gggaccagga cccaccagag cggggggctc cacccagcac cttcaaaggg
     4621 acacctacgg cagagaaccc agagtacctg ggtctggacg tgccagtgac cggttctggt
     4681 gggtcaggag ggggcggagg taagcttggt accatggtga gcaagggcga ggagctgttc
     4741 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     4801 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     4861 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     4921 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4981 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5041 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5101 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5161 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5221 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5281 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5341 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5401 atcactctcg gcatggacga gctgtacaag gaattctaac tcgagtctag agggcccgtt
     5461 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5521 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5581 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5641 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5701 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5761 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5821 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5881 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5941 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6001 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6061 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6121 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6181 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6241 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6541 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     6601 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6661 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6721 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6781 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6841 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6901 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6961 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7021 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7081 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7141 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7201 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7261 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7321 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7381 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7441 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7501 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7681 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7741 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7801 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7861 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8821 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8881 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9001 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9721 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9781 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9841 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
