LOCUS       sequence-22743-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p404GAL1
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     400..423
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     444..460
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     444..460
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(494..510)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(495..511)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(529..547)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg
       61 gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct
      121 acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac
      181 cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc
      241 ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa
      301 atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt
      361 cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac
      421 gtcaaggaga aaaaaccccg gattctagaa ctagtggatc ccccgggctg caggaattcg
      481 atatcaagct tatcgatacc gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca
      541 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
      601 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
      661 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
      721 gcttga
//
