LOCUS       sequence-227451-       10125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1 integration plasmid for cuO array with Puro selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, 
            Lomvardas S, Weiner OD
  TITLE     Live-cell imaging reveals enhancer-dependent Sox2 transcription in 
            the absence of enhancer proximity.
  JOURNAL   Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
  PUBMED    31124784
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-24"; volume: "8. pii: 41769. doi"; pages: " 
            10.7554/eLife.41769"
COMMENT     Sequence Label: pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
FEATURES             Location/Qualifiers
     source          1..10125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    83..108
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    119..144
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    155..180
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    191..216
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    227..252
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    298..323
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    334..359
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    370..395
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    406..431
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    442..467
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    478..503
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    514..539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    551..576
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    587..612
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    623..648
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    659..684
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    695..720
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    731..756
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    767..792
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    803..828
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    839..864
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    875..900
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    946..971
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    982..1007
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1018..1043
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1054..1079
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1090..1115
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1126..1151
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1162..1187
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1199..1224
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1235..1260
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1271..1296
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1307..1332
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1343..1368
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1379..1404
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1415..1440
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1451..1476
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1487..1512
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1523..1548
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1594..1619
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1630..1655
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1666..1691
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1702..1727
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1738..1763
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1774..1799
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1810..1835
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1847..1872
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1883..1908
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1919..1944
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1955..1980
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1991..2016
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2027..2052
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2063..2088
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2099..2124
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2135..2160
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2171..2196
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2242..2267
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2278..2303
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2314..2339
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2350..2375
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2386..2411
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2422..2447
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2458..2483
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2495..2520
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2531..2556
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2567..2592
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2603..2628
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2639..2664
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2718..2743
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2754..2779
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2790..2815
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2826..2851
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2862..2887
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2933..2958
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2969..2994
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3005..3030
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3041..3066
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3077..3102
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3113..3138
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3149..3174
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3186..3211
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3222..3247
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3258..3283
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3294..3319
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3330..3355
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3366..3391
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3402..3427
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3438..3463
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3474..3499
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3510..3535
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3581..3606
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3617..3642
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3653..3678
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3689..3714
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3725..3750
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3761..3786
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3797..3822
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3834..3859
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3870..3895
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3906..3931
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3942..3967
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3978..4003
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4014..4039
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4050..4075
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4086..4111
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4122..4147
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4158..4183
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4229..4254
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4265..4290
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4301..4326
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4337..4362
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4373..4398
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4409..4434
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4445..4470
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4482..4507
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4518..4543
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4554..4579
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4590..4615
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4626..4651
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4662..4687
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4698..4723
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4734..4759
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4770..4795
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4806..4831
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4877..4902
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4913..4938
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4949..4974
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4985..5010
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5021..5046
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5057..5082
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5093..5118
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5130..5155
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5166..5191
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5202..5227
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5238..5263
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5274..5299
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    complement(5317..5341)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     5364..5380
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5365..5381
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5434..5483
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    complement(5488..5521)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6029..6046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6200..6788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6280..6299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6959..7750,7751..7819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7582..7601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7820..7924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7951..8406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8038..8057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8248..8269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8530..8547
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8531..8547
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8616..9115
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8651..8674)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9048..9067
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9127..9174
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9193..9792
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9193..9212)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9689..9709
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(9824..9841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9830..10054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(10075..10108)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatcacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg
       61 gtaccgggcc ccccctcgag aaagaaacaa accaacctgt ctgtattatc aagcctgata
      121 atacagacag gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct
      181 ttaagcctga taatacagac aggttggttt gtttcttttt ctcagataat acagacaggt
      241 tggtttgttt ctttaagcct gataatacag acagttggtt tgtttctttt tctcagataa
      301 tacagacagg ttggtttgtt tctttaagcc tgataataca gacaggttgg tttgtttctt
      361 tttctcagat aatacagaca ggttggtttg tttctttaag cctgataata cagacaggtt
      421 ggtttgtttc tttttctcag ataatacaga caggttggtt tgtttcttta agcctgataa
      481 tacagacagg ttggtttgtt tctttttctc agataataca gacaggttgg tttgtttctt
      541 ttaagcctga taatacagac aggttggttt gtttcttttt ctcagataat acagacaggt
      601 tggtttgttt ctttaagcct gataatacag acaggttggt ttgtttcttt ttctcagata
      661 atacagacag gttggtttgt ttctttaagc ctgataatac agacaggttg gtttgtttct
      721 ttctcgacaa agaaacaaac caacctgtct gtattatcaa gcctgataat acagacaggt
      781 tggtttgttt ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata
      841 atacagacag gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct
      901 ttaagcctga taatacagac agttggtttg tttctttttc tcagataata cagacaggtt
      961 ggtttgtttc tttaagcctg ataatacaga caggttggtt tgtttctttt tctcagataa
     1021 tacagacagg ttggtttgtt tctttaagcc tgataataca gacaggttgg tttgtttctt
     1081 tttctcagat aatacagaca ggttggtttg tttctttaag cctgataata cagacaggtt
     1141 ggtttgtttc tttttctcag ataatacaga caggttggtt tgtttctttt aagcctgata
     1201 atacagacag gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct
     1261 ttaagcctga taatacagac aggttggttt gtttcttttt ctcagataat acagacaggt
     1321 tggtttgttt ctttaagcct gataatacag acaggttggt ttgtttcttt ctcgacaaag
     1381 aaacaaacca acctgtctgt attatcaagc ctgataatac agacaggttg gtttgtttct
     1441 ttttctcaga taatacagac aggttggttt gtttctttaa gcctgataat acagacaggt
     1501 tggtttgttt ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata
     1561 atacagacag ttggtttgtt tctttttctc agataataca gacaggttgg tttgtttctt
     1621 taagcctgat aatacagaca ggttggtttg tttctttttc tcagataata cagacaggtt
     1681 ggtttgtttc tttaagcctg ataatacaga caggttggtt tgtttctttt tctcagataa
     1741 tacagacagg ttggtttgtt tctttaagcc tgataataca gacaggttgg tttgtttctt
     1801 tttctcagat aatacagaca ggttggtttg tttcttttaa gcctgataat acagacaggt
     1861 tggtttgttt ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata
     1921 atacagacag gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct
     1981 ttaagcctga taatacagac aggttggttt gtttctttct cgacaaagaa acaaaccaac
     2041 ctgtctgtat tatcaagcct gataatacag acaggttggt ttgtttcttt ttctcagata
     2101 atacagacag gttggtttgt ttctttaagc ctgataatac agacaggttg gtttgtttct
     2161 ttttctcaga taatacagac aggttggttt gtttctttaa gcctgataat acagacagtt
     2221 ggtttgtttc tttttctcag ataatacaga caggttggtt tgtttcttta agcctgataa
     2281 tacagacagg ttggtttgtt tctttttctc agataataca gacaggttgg tttgtttctt
     2341 taagcctgat aatacagaca ggttggtttg tttctttttc tcagataata cagacaggtt
     2401 ggtttgtttc tttaagcctg ataatacaga caggttggtt tgtttctttt tctcagataa
     2461 tacagacagg ttggtttgtt tcttttaagc ctgataatac agacaggttg gtttgtttct
     2521 ttttctcaga taatacagac aggttggttt gtttctttaa gcctgataat acagacaggt
     2581 tggtttgttt ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata
     2641 atacagacag gttggtttgt ttctttgtcg acctagtcta gagcggccgc ctagcgtacc
     2701 gggccccccc tcgagaaaga aacaaaccaa cctgtctgta ttatcaagcc tgataataca
     2761 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
     2821 cctgataata cagacaggtt ggtttgtttc tttttctcag ataatacaga caggttggtt
     2881 tgtttcttta agcctgataa tacagacagt tggtttgttt ctttttctca gataatacag
     2941 acaggttggt ttgtttcttt aagcctgata atacagacag gttggtttgt ttctttttct
     3001 cagataatac agacaggttg gtttgtttct ttaagcctga taatacagac aggttggttt
     3061 gtttcttttt ctcagataat acagacaggt tggtttgttt ctttaagcct gataatacag
     3121 acaggttggt ttgtttcttt ttctcagata atacagacag gttggtttgt ttcttttaag
     3181 cctgataata cagacaggtt ggtttgtttc tttttctcag ataatacaga caggttggtt
     3241 tgtttcttta agcctgataa tacagacagg ttggtttgtt tctttttctc agataataca
     3301 gacaggttgg tttgtttctt taagcctgat aatacagaca ggttggtttg tttctttctc
     3361 gacaaagaaa caaaccaacc tgtctgtatt atcaagcctg ataatacaga caggttggtt
     3421 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     3481 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
     3541 cctgataata cagacagttg gtttgtttct ttttctcaga taatacagac aggttggttt
     3601 gtttctttaa gcctgataat acagacaggt tggtttgttt ctttttctca gataatacag
     3661 acaggttggt ttgtttcttt aagcctgata atacagacag gttggtttgt ttctttttct
     3721 cagataatac agacaggttg gtttgtttct ttaagcctga taatacagac aggttggttt
     3781 gtttcttttt ctcagataat acagacaggt tggtttgttt cttttaagcc tgataataca
     3841 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
     3901 cctgataata cagacaggtt ggtttgtttc tttttctcag ataatacaga caggttggtt
     3961 tgtttcttta agcctgataa tacagacagg ttggtttgtt tctttctcga caaagaaaca
     4021 aaccaacctg tctgtattat caagcctgat aatacagaca ggttggtttg tttctttttc
     4081 tcagataata cagacaggtt ggtttgtttc tttaagcctg ataatacaga caggttggtt
     4141 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     4201 gacagttggt ttgtttcttt ttctcagata atacagacag gttggtttgt ttctttaagc
     4261 ctgataatac agacaggttg gtttgtttct ttttctcaga taatacagac aggttggttt
     4321 gtttctttaa gcctgataat acagacaggt tggtttgttt ctttttctca gataatacag
     4381 acaggttggt ttgtttcttt aagcctgata atacagacag gttggtttgt ttctttttct
     4441 cagataatac agacaggttg gtttgtttct tttaagcctg ataatacaga caggttggtt
     4501 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     4561 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
     4621 cctgataata cagacaggtt ggtttgtttc tttctcgaca aagaaacaaa ccaacctgtc
     4681 tgtattatca agcctgataa tacagacagg ttggtttgtt tctttttctc agataataca
     4741 gacaggttgg tttgtttctt taagcctgat aatacagaca ggttggtttg tttctttttc
     4801 tcagataata cagacaggtt ggtttgtttc tttaagcctg ataatacaga cagttggttt
     4861 gtttcttttt ctcagataat acagacaggt tggtttgttt ctttaagcct gataatacag
     4921 acaggttggt ttgtttcttt ttctcagata atacagacag gttggtttgt ttctttaagc
     4981 ctgataatac agacaggttg gtttgtttct ttttctcaga taatacagac aggttggttt
     5041 gtttctttaa gcctgataat acagacaggt tggtttgttt ctttttctca gataatacag
     5101 acaggttggt ttgtttcttt taagcctgat aatacagaca ggttggtttg tttctttttc
     5161 tcagataata cagacaggtt ggtttgtttc tttaagcctg ataatacaga caggttggtt
     5221 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     5281 gacaggttgg tttgtttctt tgtcgaccta gtctagaccc agctttcttg tacaaagtgg
     5341 ttgatgggga tccgaattcg agctcgaggt cgacggtatc gataagcttg atatcgaatt
     5401 cctgcagccc gggcagggat ccactagttc tagtcggccg gcttgtcgac gacggcggtc
     5461 tccgtcgtca ggatcatccg ggcctagata acttcgtata gcatacatta tacgaagtta
     5521 tgcggcttcg agaccgtgac ctacatcgtc gacgcggcca gatggtaagc cctcccgtat
     5581 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5641 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6001 gtagccgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6601 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7921 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     7981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8341 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     8401 caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8461 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8521 cgccagggtt gtaaaacgac ggccagtgaa ttcgagctcg gtacgagttt acgtccagcc
     8581 aagctagctt ggctgcaggt cgtcgaaatt ctaccgggta ggggaggcgc ttttcccaag
     8641 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     8701 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     8761 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     8821 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     8881 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     8941 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     9001 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     9061 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     9121 cagcctgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     9181 aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     9241 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     9301 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     9361 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     9421 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     9481 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     9541 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     9601 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     9661 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     9721 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9781 cccggtgcct gaggggatca attctctaga gctcgctgat cagcctcgac tgtgccttct
     9841 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9901 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9961 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    10021 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccataact
    10081 tcgtatagca tacattatac gaagttatgt accgggcccc atcaa
//
