LOCUS       sequence-227452-       15058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhiC31 integration plasmid for tetO array with GFP expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15058)
  AUTHORS   Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, 
            Lomvardas S, Weiner OD
  TITLE     Live-cell imaging reveals enhancer-dependent Sox2 transcription in 
            the absence of enhancer proximity.
  JOURNAL   Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
  PUBMED    31124784
REFERENCE   2  (bases 1 to 15058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-24"; volume: "8. pii: 41769. doi"; pages: " 
            10.7554/eLife.41769"
COMMENT     Sequence Label: pDEST-tetOx224_PhiC31attB_FRT-EF1aGFP-FRT
FEATURES             Location/Qualifiers
     source          1..15058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(166..184,185..207,208..226,227..249,250..268,269..291,
                     292..310,311..333,334..352,353..375,376..394,395..417,
                     418..436)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(513..531,532..554,555..573,574..596,597..615,616..638,
                     639..657,658..680,681..699,700..722,723..741,742..764,
                     765..783)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(860..878,879..901,902..920,921..943,944..962,963..985,
                     986..1004,1005..1027,1028..1046,1047..1069,1070..1088,
                     1089..1111,1112..1130)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1207..1225,1226..1248,1249..1267,1268..1290,1291..1309,
                     1310..1332,1333..1351,1352..1374,1375..1393,1394..1416,
                     1417..1435,1436..1458,1459..1477)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1554..1572,1573..1595,1596..1614,1615..1637,1638..1656,
                     1657..1679,1680..1698,1699..1721,1722..1740,1741..1763,
                     1764..1782,1783..1805,1806..1824)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1901..1919,1920..1942,1943..1961,1962..1984,1985..2003,
                     2004..2026,2027..2045,2046..2068,2069..2087,2088..2110,
                     2111..2129,2130..2152,2153..2171)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2248..2266,2267..2289,2290..2308,2309..2331,2332..2350,
                     2351..2373,2374..2392,2393..2415,2416..2434,2435..2457,
                     2458..2476,2477..2499,2500..2518)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2595..2613,2614..2636,2637..2655,2656..2678,2679..2697,
                     2698..2720,2721..2739,2740..2762,2763..2781,2782..2804,
                     2805..2823,2824..2846,2847..2865)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2942..2960,2961..2983,2984..3002,3003..3025,3026..3044,
                     3045..3067,3068..3086,3087..3109,3110..3128,3129..3151,
                     3152..3170,3171..3193,3194..3212)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3289..3307,3308..3330,3331..3349,3350..3372,3373..3391,
                     3392..3414,3415..3433,3434..3456,3457..3475,3476..3498,
                     3499..3517,3518..3540,3541..3559)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3636..3654,3655..3677,3678..3696,3697..3719,3720..3738,
                     3739..3761,3762..3780,3781..3803,3804..3822,3823..3845,
                     3846..3864,3865..3887,3888..3906)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3983..4001,4002..4024,4025..4043,4044..4066,4067..4085,
                     4086..4108,4109..4127,4128..4150,4151..4169,4170..4192,
                     4193..4211,4212..4234,4235..4253)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4330..4348,4349..4371,4372..4390,4391..4413,4414..4432,
                     4433..4455,4456..4474,4475..4497,4498..4516,4517..4539,
                     4540..4558,4559..4581,4582..4600)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4677..4695,4696..4718,4719..4737,4738..4760,4761..4779,
                     4780..4802,4803..4821,4822..4844,4845..4863,4864..4886,
                     4887..4905,4906..4928,4929..4947)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5024..5042,5043..5065,5066..5084,5085..5107,5108..5126,
                     5127..5149,5150..5168,5169..5191,5192..5210,5211..5233,
                     5234..5252,5253..5275,5276..5294)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5371..5389,5390..5412,5413..5431,5432..5454,5455..5473,
                     5474..5496,5497..5515,5516..5538,5539..5557,5558..5580,
                     5581..5599,5600..5622,5623..5641)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5718..5736,5737..5759,5760..5778,5779..5801,5802..5820,
                     5821..5843,5844..5862,5863..5885,5886..5904,5905..5927,
                     5928..5946,5947..5969,5970..5988)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6065..6083,6084..6106,6107..6125,6126..6148,6149..6167,
                     6168..6190,6191..6209,6210..6232,6233..6251,6252..6274,
                     6275..6293,6294..6316,6317..6335)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6412..6430,6431..6453,6454..6472,6473..6495,6496..6514,
                     6515..6537,6538..6556,6557..6579,6580..6598,6599..6621,
                     6622..6640,6641..6663,6664..6682)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6759..6777,6778..6800,6801..6819,6820..6842,6843..6861,
                     6862..6884,6885..6903,6904..6926,6927..6945,6946..6968,
                     6969..6987,6988..7010,7011..7029)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7106..7124,7125..7147,7148..7166,7167..7189,7190..7208,
                     7209..7231,7232..7250,7251..7273,7274..7292,7293..7315,
                     7316..7334,7335..7357,7358..7376)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7453..7471,7472..7494,7495..7513,7514..7536,7537..7555,
                     7556..7578,7579..7597,7598..7620,7621..7639,7640..7662,
                     7663..7681,7682..7704,7705..7723)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7800..7818,7819..7841,7842..7860,7861..7883,7884..7902,
                     7903..7925,7926..7944,7945..7967,7968..7986,7987..8009,
                     8010..8028,8029..8051,8052..8070)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8147..8165,8166..8188,8189..8207,8208..8230,8231..8249,
                     8250..8272,8273..8291,8292..8314,8315..8333,8334..8356,
                     8357..8375,8376..8398,8399..8417)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8494..8512,8513..8535,8536..8554,8555..8577,8578..8596,
                     8597..8619,8620..8638,8639..8661,8662..8680,8681..8703,
                     8704..8722,8723..8745,8746..8764)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8841..8859,8860..8882,8883..8901,8902..8924,8925..8943,
                     8944..8966,8967..8985,8986..9008,9009..9027,9028..9050,
                     9051..9069,9070..9092,9093..9111)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(9144..9160)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9161..9185)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     9209..9225
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9262..9278)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9496..9565
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(10062..10079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(10177..10210)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(10271..10859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10351..10370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11030..11821,11822..11890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11653..11672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11891..11995)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12022..12477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12109..12128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12319..12340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12604..12626
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12618..12635
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12619..12635
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    12705..12929
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12918..12935
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(12965..13678,13679..13681,13682..13684))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(12968..13678,13679..13681,13682..13684))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13010..13031)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     13359..13378
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13618..13639
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      13681..13690
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(13697..14874)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          13706..14643
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(13729..13749)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(14991..15024)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    join(15058,1..24)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
       61 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      121 ggatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      361 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      421 ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac
      481 gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa
      541 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      601 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      661 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      721 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      781 agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt
      841 cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      901 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      961 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     1021 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     1081 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     1141 tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt
     1201 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1261 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1321 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     1381 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1441 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc
     1501 ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat
     1561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     1621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     1681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     1741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     1801 accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc
     1861 cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa
     1921 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     1981 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2041 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2101 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2161 agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc
     2221 cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2281 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2341 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2401 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2461 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2521 agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct ttcgtcttca
     2581 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2641 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2701 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2761 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2821 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcggg
     2881 ctcggttccc gggtcgagat ccgtatcacg aggccctttc gtcttcactc gagtttacca
     2941 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3001 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     3061 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     3121 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     3181 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc ggttcccggg
     3241 tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc cctatcagtg
     3301 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     3361 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     3421 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     3481 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     3541 tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat
     3601 cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg
     3661 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     3721 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     3781 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3841 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     3901 tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt
     3961 cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     4021 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     4081 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     4141 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     4201 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     4261 aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga
     4321 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     4381 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     4441 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     4501 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     4561 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg
     4621 ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc
     4681 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     4741 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     4801 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     4861 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     4921 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag
     4981 atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat cagtgataga
     5041 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     5101 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     5161 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     5221 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     5281 atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga
     5341 ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     5401 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     5461 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     5521 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     5581 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     5641 aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc cctttcgtct
     5701 tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5761 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5821 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5881 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     5941 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     6001 gggctcggtt cccgggtcga gatccgtatc acgaggccct ttcgtcttca ctcgagttta
     6061 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     6121 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     6181 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     6241 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     6301 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcggg ctcggttccc
     6361 gggtcgagat ccgtatcacg aggccctttc gtcttcactc gagtttacca ctccctatca
     6421 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     6481 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     6541 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     6601 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     6661 cactccctat cagtgataga gaaaagtgaa agtcgggctc ggttcccggg tcgagatccg
     6721 tatcacgagg ccctttcgtc ttcactcgag tttaccactc cctatcagtg atagagaaaa
     6781 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     6841 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6901 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     6961 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     7021 tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat cacgaggccc
     7081 tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     7141 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     7201 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     7261 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     7321 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     7381 tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt cgtcttcact
     7441 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     7501 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     7561 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     7621 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     7681 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgggct
     7741 cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga gtttaccact
     7801 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     7861 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     7921 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     7981 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     8041 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg ttcccgggtc
     8101 gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc tatcagtgat
     8161 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     8221 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     8281 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     8341 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     8401 cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag atccgtatca
     8461 cgaggccctt tcgtcttcac tcgagtttac cactccctat cagtgataga gaaaagtgaa
     8521 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     8581 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     8641 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     8701 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     8761 gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga ggccctttcg
     8821 tcttcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     8881 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     8941 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     9001 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     9061 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     9121 gtcgggctcg gttcccgggt cgagatccac tagttctaga acccagcttt cttgtacaaa
     9181 gtggttgatg gggatccgaa ttcgagctcg aggtcgacgg tatcgataag cttgatatcg
     9241 aattcctgca gcccgggcag ggatccacta gttctagaat tcgagctccc gggtactcga
     9301 ggtcgacatg cccgccgtga ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc
     9361 cgccgacgcc gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg
     9421 cgagggcttc ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt
     9481 catcatgatg gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc
     9541 ctggcacccg caccgcggct tcgagaccgt gacctacatc gtcgacgcgg ccagatggta
     9601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10021 ctgttcttct agtgtagccg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10081 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10141 tccacagaat caggggataa cgcaggaaag aacatggaag ttcctatact ttctagagaa
    10201 taggaacttc catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    10741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    11041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    11101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    11161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    11221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    11281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11341 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11641 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    12001 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    12061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    12121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    12181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    12241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    12301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    12361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    12421 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc
    12481 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    12541 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    12601 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acagggttcc
    12661 gcaagctcta gtcgagcccc agctggttct ttccgcctca gaagccatag agcccaccgc
    12721 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
    12781 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
    12841 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac
    12901 aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgagctc tagagtcgac
    12961 ttaattactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
    13021 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
    13081 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
    13141 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
    13201 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
    13261 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
    13321 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
    13381 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
    13441 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
    13501 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
    13561 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
    13621 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
    13681 ccatggtggc cgtacgtcac gacacctgaa atggaagaaa aaaactttga accactgtct
    13741 gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag gagaattaca
    13801 tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga aactccatcg
    13861 cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagctcgag aactaatcga
    13921 ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct gaggacggaa
    13981 aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc
    14041 attttgagct ccctgcagca gggccgggaa gcggccatct ttccgctcac gcaactggtg
    14101 ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg aggccaggca
    14161 ccagagcagg ccggccagct tgagactacc cccgtccgat tctcggtggc cgcgctcgca
    14221 ggccccgcct cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc
    14281 caaaaaccga aataccagtg tgcagatctt ggcccgcatt tacaagacta tcttgccaga
    14341 aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc tagagactta tcgaaagcag
    14401 cgagacaggc gcgaaggtgc caccagattc gcacgcggcg gccccagcgc ccaggccagg
    14461 cctcaactca agcacgaggc gaaggggctc cttaagcgca aggcctcgaa ctctcccacc
    14521 cacttccaac ccgaagctcg ggatcaagaa tcacgtactg cagccagtgg aagtaattca
    14581 aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac acacggcact
    14641 tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact
    14701 tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc
    14761 ccagtttacc ccgcgccacc ttctctaggc accggatcaa ttgccgaccc ctccccccaa
    14821 cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg agccaattcc
    14881 cactcctttc aagacctaga aggtccatta gctgcaaaga ttcctctctg tttaaaactt
    14941 tatccatctt tgcagctagc ttggctggac gtaaactcct cttcagacct gaagttccta
    15001 tactttctag agaataggaa cttcggaatt cgatatcaag cgtaccgggc cccatcaa
//
