LOCUS       sequence-227453-       14953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhiC31 integration plasmid for tetO array with Neo selection 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, 
            Lomvardas S, Weiner OD
  TITLE     Live-cell imaging reveals enhancer-dependent Sox2 transcription in 
            the absence of enhancer proximity.
  JOURNAL   Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
  PUBMED    31124784
REFERENCE   2  (bases 1 to 14953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-24"; volume: "8. pii: 41769. doi"; pages: " 
            10.7554/eLife.41769"
COMMENT     Sequence Label: pDEST-tetOx224_PhiC31attB_FRT-PGKneo-FRT
FEATURES             Location/Qualifiers
     source          1..14953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    279..297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    390..408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    432..450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    474..492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    516..534
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    558..576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    join(653..671,672..694,695..713,714..736,737..755,756..778,
                     779..797,798..820,821..839,840..862,863..881,882..904,
                     905..923)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1000..1018,1019..1041,1042..1060,1061..1083,1084..1102,
                     1103..1125,1126..1144,1145..1167,1168..1186,1187..1209,
                     1210..1228,1229..1251,1252..1270)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1347..1365,1366..1388,1389..1407,1408..1430,1431..1449,
                     1450..1472,1473..1491,1492..1514,1515..1533,1534..1556,
                     1557..1575,1576..1598,1599..1617)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1694..1712,1713..1735,1736..1754,1755..1777,1778..1796,
                     1797..1819,1820..1838,1839..1861,1862..1880,1881..1903,
                     1904..1922,1923..1945,1946..1964)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2041..2059,2060..2082,2083..2101,2102..2124,2125..2143,
                     2144..2166,2167..2185,2186..2208,2209..2227,2228..2250,
                     2251..2269,2270..2292,2293..2311)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2388..2406,2407..2429,2430..2448,2449..2471,2472..2490,
                     2491..2513,2514..2532,2533..2555,2556..2574,2575..2597,
                     2598..2616,2617..2639,2640..2658)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2735..2753,2754..2776,2777..2795,2796..2818,2819..2837,
                     2838..2860,2861..2879,2880..2902,2903..2921,2922..2944,
                     2945..2963,2964..2986,2987..3005)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3082..3100,3101..3123,3124..3142,3143..3165,3166..3184,
                     3185..3207,3208..3226,3227..3249,3250..3268,3269..3291,
                     3292..3310,3311..3333,3334..3352)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3429..3447,3448..3470,3471..3489,3490..3512,3513..3531,
                     3532..3554,3555..3573,3574..3596,3597..3615,3616..3638,
                     3639..3657,3658..3680,3681..3699)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3776..3794,3795..3817,3818..3836,3837..3859,3860..3878,
                     3879..3901,3902..3920,3921..3943,3944..3962,3963..3985,
                     3986..4004,4005..4027,4028..4046)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4123..4141,4142..4164,4165..4183,4184..4206,4207..4225,
                     4226..4248,4249..4267,4268..4290,4291..4309,4310..4332,
                     4333..4351,4352..4374,4375..4393)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4470..4488,4489..4511,4512..4530,4531..4553,4554..4572,
                     4573..4595,4596..4614,4615..4637,4638..4656,4657..4679,
                     4680..4698,4699..4721,4722..4740)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4817..4835,4836..4858,4859..4877,4878..4900,4901..4919,
                     4920..4942,4943..4961,4962..4984,4985..5003,5004..5026,
                     5027..5045,5046..5068,5069..5087)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5164..5182,5183..5205,5206..5224,5225..5247,5248..5266,
                     5267..5289,5290..5308,5309..5331,5332..5350,5351..5373,
                     5374..5392,5393..5415,5416..5434)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5511..5529,5530..5552,5553..5571,5572..5594,5595..5613,
                     5614..5636,5637..5655,5656..5678,5679..5697,5698..5720,
                     5721..5739,5740..5762,5763..5781)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5858..5876,5877..5899,5900..5918,5919..5941,5942..5960,
                     5961..5983,5984..6002,6003..6025,6026..6044,6045..6067,
                     6068..6086,6087..6109,6110..6128)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6205..6223,6224..6246,6247..6265,6266..6288,6289..6307,
                     6308..6330,6331..6349,6350..6372,6373..6391,6392..6414,
                     6415..6433,6434..6456,6457..6475)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6552..6570,6571..6593,6594..6612,6613..6635,6636..6654,
                     6655..6677,6678..6696,6697..6719,6720..6738,6739..6761,
                     6762..6780,6781..6803,6804..6822)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6899..6917,6918..6940,6941..6959,6960..6982,6983..7001,
                     7002..7024,7025..7043,7044..7066,7067..7085,7086..7108,
                     7109..7127,7128..7150,7151..7169)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7246..7264,7265..7287,7288..7306,7307..7329,7330..7348,
                     7349..7371,7372..7390,7391..7413,7414..7432,7433..7455,
                     7456..7474,7475..7497,7498..7516)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7593..7611,7612..7634,7635..7653,7654..7676,7677..7695,
                     7696..7718,7719..7737,7738..7760,7761..7779,7780..7802,
                     7803..7821,7822..7844,7845..7863)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7940..7958,7959..7981,7982..8000,8001..8023,8024..8042,
                     8043..8065,8066..8084,8085..8107,8108..8126,8127..8149,
                     8150..8168,8169..8191,8192..8210)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8287..8305,8306..8328,8329..8347,8348..8370,8371..8389,
                     8390..8412,8413..8431,8432..8454,8455..8473,8474..8496,
                     8497..8515,8516..8538,8539..8557)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8634..8652,8653..8675,8676..8694,8695..8717,8718..8736,
                     8737..8759,8760..8778,8779..8801,8802..8820,8821..8843,
                     8844..8862,8863..8885,8886..8904)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8981..8999,9000..9022,9023..9041,9042..9064,9065..9083,
                     9084..9106,9107..9125,9126..9148,9149..9167,9168..9190,
                     9191..9209,9210..9232,9233..9251)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9328..9346,9347..9369,9370..9388,9389..9411,9412..9430,
                     9431..9453,9454..9472,9473..9495,9496..9514,9515..9537,
                     9538..9556,9557..9579,9580..9598)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(9631..9647)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9648..9672)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     9696..9712
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9749..9765)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9983..10052
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(10549..10566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(10664..10697)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(10758..11346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10838..10857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11517..12308,12309..12377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12140..12159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12378..12482)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12509..12964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12596..12615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12806..12827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13091..13113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13105..13122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13106..13122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    13192..13416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13405..13422
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(13454..14257)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13565..13584)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14175..14194
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(14276..14323)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(14335..14822)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(14383..14402)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     14764..14787
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(14886..14919)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    join(14953,1..24)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
       61 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      121 gggatcccga agaagtacgt agagaggaaa aggcgatcgc agaaggagaa aggctcttca
      181 agggatggag aagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc
      241 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      301 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt catcagtgat
      361 agagaaaagt gaaagtcgag tttaccactt ccctatcagt gatagagaaa agtgaaagtc
      421 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      481 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      541 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc
      601 cgggtcgaga tccgtatcac gaggcccttt cgtcttcact cgagtttacc actccctatc
      661 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      721 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      781 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      841 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      901 ccactcccta tcagtgatag agaaaagtga aagtcgggct cggttcccgg gtcgagatcc
      961 gtatcacgag gccctttcgt cttcactcga gtttaccact ccctatcagt gatagagaaa
     1021 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     1081 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     1141 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     1201 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     1261 gtgatagaga aaagtgaaag tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc
     1321 ctttcgtctt cactcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     1381 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     1441 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     1501 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     1561 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     1621 gtgaaagtcg ggctcggttc ccgggtcgag atccgtatca cgaggccctt tcgtcttcac
     1681 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     1741 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1801 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     1861 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     1921 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgggc
     1981 tcggttcccg ggtcgagatc cgtatcacga ggccctttcg tcttcactcg agtttaccac
     2041 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2101 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2161 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2221 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt
     2341 cgagatccgt atcacgaggc cctttcgtct tcactcgagt ttaccactcc ctatcagtga
     2401 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2461 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2521 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2581 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2641 ccctatcagt gatagagaaa agtgaaagtc gggctcggtt cccgggtcga gatccgtatc
     2701 acgaggccct ttcgtcttca ctcgagttta ccactcccta tcagtgatag agaaaagtga
     2761 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2821 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2881 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2941 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     3001 agagaaaagt gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg aggccctttc
     3061 gtcttcactc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     3121 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     3181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     3241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     3301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     3361 agtcgggctc ggttcccggg tcgagatccg tatcacgagg ccctttcgtc ttcactcgag
     3421 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     3481 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     3541 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     3601 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     3661 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgggctcggt
     3721 tcccgggtcg agatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct
     3781 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     3841 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3901 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3961 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     4021 ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga
     4081 tccgtatcac gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag
     4141 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     4201 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     4261 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     4321 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     4381 tcagtgatag agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag
     4441 gccctttcgt cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4501 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4741 aaagtgaaag tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt
     4801 cactcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     4861 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     4921 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     4981 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     5041 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     5101 ggctcggttc ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac
     5161 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     5221 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     5281 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     5341 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     5401 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg
     5461 ggtcgagatc cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag
     5521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     5641 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     5701 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     5761 actccctatc agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt
     5821 atcacgaggc cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag
     5881 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     5941 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     6001 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     6061 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     6121 gatagagaaa agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct
     6181 ttcgtcttca ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     6241 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     6301 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     6361 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     6421 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     6481 gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg aggccctttc gtcttcactc
     6541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     6601 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     6661 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     6721 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     6781 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc
     6841 ggttcccggg tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc
     6901 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     6961 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     7021 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     7081 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     7141 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg
     7201 agatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata
     7261 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     7321 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     7381 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     7441 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     7501 ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac
     7561 gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa
     7621 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     7681 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     7741 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     7801 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     7861 agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt
     7921 cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     7981 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     8041 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     8101 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     8161 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     8221 tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt
     8281 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     8341 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     8401 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     8461 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     8521 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc
     8581 ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat
     8641 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     8701 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     8761 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     8821 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     8881 accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc
     8941 cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa
     9001 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     9061 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     9121 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9181 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9241 agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc
     9301 cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     9361 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     9421 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     9481 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     9541 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     9601 agtgaaagtc gggctcggtt cccgggtcga gatccactag ttctagaacc cagctttctt
     9661 gtacaaagtg gttgatgggg atccgaattc gagctcgagg tcgacggtat cgataagctt
     9721 gatatcgaat tcctgcagcc cgggcaggga tccactagtt ctagaattcg agctcccggg
     9781 tactctaggt cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat
     9841 ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt
     9901 tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg
     9961 acccgttcat catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg
    10021 gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc gacgcggcca
    10081 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10141 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10201 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10261 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10321 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10381 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10441 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10501 ccaaatactg ttcttctagt gtagccgtat tgggcgctct tccgcttcct cgctcactga
    10561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10621 acggttatcc acagaatcag gggataacgc aggaaagaac atggaagttc ctatactttc
    10681 tagagaatag gaacttccat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10741 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11161 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    11221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11341 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11401 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11461 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11521 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11581 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11641 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11701 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11761 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11821 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11881 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11941 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    12001 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    12061 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    12121 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    12181 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    12241 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12301 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12361 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12421 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12481 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
    12541 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    12601 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    12661 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    12721 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    12781 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    12841 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    12901 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
    12961 atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    13021 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    13081 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtaca
    13141 gggttccgca agctctagtc gagccccagc tggttctttc cgcctcagaa gccatagagc
    13201 ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
    13261 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
    13321 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
    13381 ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag
    13441 agaattgatc ccctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
    13501 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
    13561 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
    13621 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
    13681 cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg cgccttgagc ctggcgaaca
    13741 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
    13801 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
    13861 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
    13921 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
    13981 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
    14041 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
    14101 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
    14161 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
    14221 ctgcgtgcaa tccatcttgt tcaatggccg atcccatggt ttagttcctc accttgtcgt
    14281 attatactat gccgatatac tatgccgatg attaattgtc aacaggctgc aggtcgaaag
    14341 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
    14401 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccccggaccc
    14461 accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt ggccgctgcc
    14521 ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga gactagtgag
    14581 acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg gggggaactt
    14641 cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg
    14701 gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt
    14761 gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac
    14821 ccggtagaat ttcgacgacc tgcagccaag ctagcttggc tggacgtaaa ctcctcttca
    14881 gacctgaagt tcctatactt tctagagaat aggaacttcg gaattcgata tcaagcgtac
    14941 cgggccccat caa
//
