LOCUS       sequence-227486-       10580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW683 - pAHP6>>
FEATURES             Location/Qualifiers
     source          1..10580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             4118..4462
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      4469..5110
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     protein_bind    5216..5235
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5268..5287
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5320..5339
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5372..5391
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        5415..5461
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(5546..5548,5549..5551,5552..6262)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5591..5612)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5852..5871)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6199..6220
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6926..7306
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    8599..8623
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8714..9302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8794..8813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9473..10261)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(10552..10580,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta
      601 attaaacacg gcgcgccaac agcgatcgca ccaggtacca cctcacgggg cgcaaagaag
      661 catgacatac gaatgagaca atttcagttt tcgtatataa taattccata atcataattt
      721 aatcactagt tttaattggt aacataacct taaaaaagca tccactcatt atgcgctcca
      781 ctttactact tactctgata acatgaaaaa gtatatgttt aatactagtt gtttgttccg
      841 tttccaagca cgctctttct tcttttcagt catttttaat ccgcatttac aataatattt
      901 agctattttc atatatcatt ctatactagc tcacaataat tacatatgag cacgcttttt
      961 tgtgttcaaa tattataaat cttcgttagt taccgctgct ttttataatt atgtatactt
     1021 tctggttcca ggagactaat taaggaagat tattagaggc ttcttatacg tgccatatga
     1081 ttatttattt atgtactgtc atggttcata tatagcgatg tgataccaat ataccaatca
     1141 ccaaagttat gagaaaatca cgtaaccaga ggtgttggta catacatttt ctgttaaaaa
     1201 tagttaagag gacttgtaat cttttgtgtt taactattga taatttttta ctttattcgg
     1261 tgaaacaatt tacgcatgtt tctgtgttgt gcttgtgttt cttgtattct tcttgtgtat
     1321 agctttcctt ataagaaaga aaaagaagaa gattaattga ttagatcgag aaagagaaag
     1381 gccttttgag atagaaatgg agaaaggatg gattgaattt gaagcataga gaatagcgaa
     1441 cactcactgc aaagttagtg ggaaagtcgc gcgtcatacg agggagcaac aaagtgtggg
     1501 cttttctttt acagtctcag aaactaatgc atgctcacat ttataaaaaa aaaaaaaaac
     1561 aattcttgtg ttaaatcaag agtggattaa ttatgagata atctaatgta cattactgtt
     1621 atatgattat aacttgatag accaaataat catcttaatc agacctgata aaagaaaaga
     1681 gattaatttc aacagtgaga aattaaagat agaaagtaca tattattggt gtaaaagttg
     1741 ttgatttaat ttaaggcaga tgtattaagt taaatatgta aatgtagagg ctgcgggaag
     1801 ggtgaccagc tggtctgaca gggtacgccg gttgtcggga ggaagaacgc cgctattggc
     1861 agagtcacgc ggggtaaccg gctggcggct gtagccggtc acagagtgta ttaatatggt
     1921 cgtacaatac ttgtagagtg ctttttcctg tgacggtccc gtttattccc cacaccctcc
     1981 tcgattcttt cagttaaaag tgggggaaaa aggtaacctg actcttagaa aatttgaata
     2041 caattgtggg tcacctcatc tcatgtagtc atgtctatac aattataaat acctctcaac
     2101 aaacccaaaa tcgaattata agccttctct agctagagtt tgaagcaaga cctgttcaca
     2161 ttagtaaaaa caaatatata gaaagaggaa agattgaagc ggaaaacggg acaagacggg
     2221 tgtgccgttg aacacaatgg ctagtgaagc tcgaaaaaca aagaaaaaaa tcaaagggat
     2281 tcagcaagcc actgcaggag tctcacaaga cacttcggaa aatcctaaca aaacaatagt
     2341 tcctgcagca ttaccacagc tcacccctac cttggtgtca ctgctggagg tgattgaacc
     2401 cgaggtgttg tatgcaggat atgatagctc tgttccagat tcagcatgga gaattatgac
     2461 cacactcaac atgttaggtg ggcgtcaagt gattgcagca gtgaaatggg caaaggcgat
     2521 accaggcttc agaaacttac acctggatga ccaaatgacc ctgctacagt actcatggat
     2581 gtttctcatg gcatttgccc tgggttggag atcatacaga caatcaagtg gaaacctgct
     2641 ctgctttgct cctgatctga ttattaatga gcagagaatg tctctaccct gcatgtatga
     2701 ccaatgtaaa cacatgctgt ttgtctcctc tgaattacaa agattgcagg tatcctatga
     2761 agagtatctc tgtatgaaaa ccttactgct tctctcctca gttcctaagg aaggtctgaa
     2821 gagccaagag ttatttgatg agattcgaat gacttatatc aaagagctag gaaaagccat
     2881 cgtcaaaagg gaagggaact ccagtcagaa ctggcaacgg ttttaccaac tgacaaagct
     2941 tctggactcc atgcatgagg tggttgagaa tctccttacc tactgcttcc agacattttt
     3001 ggataagacc atgagtattg aattcccaga gatgttagct gaaatcatca ctaatcagat
     3061 accaaaatat tcaaatggaa atatcaaaaa gcttctgttt catcaaaaat ctactagcaa
     3121 accggtaacg ttatacgacg tcgctgaata cgccggcgtt tctcatcaaa ccgtttctag
     3181 agtggttaac caggcttcac atgttagcgc taaaacccgg gaaaaagttg aagctgccat
     3241 ggctgagctc aactacatcc cgaaccgtgt tgcgcagcag ctggctggta aacaaagctt
     3301 gctgatcggt gtcgcgacct cgagcttggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
     3361 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
     3421 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
     3481 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
     3541 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
     3601 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
     3661 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
     3721 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
     3781 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
     3841 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     3901 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     3961 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     4021 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     4081 actggtgaaa agaaaaacca ctagtggatc ggaattcgcc aattttaatc aaagtgggaa
     4141 tattgctgat agctcattgt ccttcacttt cactaacagt agcaacggtc cgaacctcat
     4201 aacaactcaa acaaattctc aagcgctttc acaaccaatt gcctcctcta acgttcatga
     4261 taacttcatg aataatgaaa tcacggctag taaaattgat gatggtaata attcaaaacc
     4321 actgtcacct ggttggacgg accaaactgc gtataacgcg tttggaatca ctacagggat
     4381 gtttaatacc actacaatgg atgatgtata taactatcta ttcgatgatg aagatacccc
     4441 accaaaccca aaaaaagagt aactgcaaag ctttcgttcg tatcatcggt ttcgacaacg
     4501 ttcgtcaagt tcaatgcatc agtttcattg cgcacacacc agaatcctac tgagtttgag
     4561 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
     4621 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
     4681 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
     4741 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
     4801 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgta
     4861 ccattatgct tattcactag gcaacaaata tattttcaga cctagaaaag ctgcaaatgt
     4921 tactgaatac aagtatgtcc tcttgtgttt tagacattta tgaactttcc tttatgtaat
     4981 tttccagaat ccttgtcaga ttctaatcat tgctttataa ttatagttat actcatggat
     5041 ttgtagttga gtatgaaaat attttttaat gcattttatg acttgccaat tgattgacaa
     5101 catgcatcaa tcgactatga gtaggcaaga tgttctgtag gggctatgat gcctggtcac
     5161 aacctatgca tatgtcgagc tcaagaaatc tagaaagaag aaagggaaga gaaagaattg
     5221 tgagcgctca caattgaaag actagaaaga agaaagggaa gagaaagaat tgtgagcgct
     5281 cacaattgaa agactagaaa gaagaaaggg aagagaaaga attgtgagcg ctcacaattg
     5341 aaagactaga aagaagaaag ggaagagaaa gaattgtgag cgctcacaat tgaaagacta
     5401 gtggatcgat cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     5461 acgaacaatg aaagccttca cactcgctct cttcttagct ctttccctct atctcctgcc
     5521 caatccagcc actagtggct caacaatggt gagcaagggc gaggagctgt tcaccggggt
     5581 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5641 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5701 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
     5761 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5821 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5881 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5941 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta
     6001 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     6061 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6121 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     6181 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6241 cggcatggac gagctgtaca agtcaggtca tgatgagctt tagctgcatc tcgtctctgt
     6301 tatgcttaag aagttcaatg tttcgtttca tgtaaaactt tggtggtttg tgttttgggg
     6361 ccttgtataa tccctgatga ataagtgttc tactatgttt ccgttcctgt tatctctttc
     6421 tttctaatga caagtcgaac ttcttcttta tcatcgcttc gtttttatta tctgtgcttc
     6481 ttttgtttaa tacgcctgca aagtgactcg actctgttta gtgcagttct gcgaaacttg
     6541 taaatagtcc aattgttggc ctctagtaat agatgtagcg aaagtgttga gctgttgggt
     6601 tctaaggatg gcttgaacat gttaatcttt taggttctga gtatgatgaa cattcgttgt
     6661 tgctaagaaa tgcctgtaat gtcccacaaa tgtagaaaat ggttcgtacc tttgtccaag
     6721 cattgatatg tctgatgaga ggaaactgca agatactgag cttggtttaa cgaaggagag
     6781 gcagtttctt ccttccaaag catttcattt gacaatgcct tgatcatctt aagtagagtt
     6841 tctgttgtgg aaagtttgaa actttgaaga aacgactctc aagtaaattg atgatcacaa
     6901 gtgaaagtgt atgttactag gatccttttc aaatcagtgc gcaagacgtg acgtaagtat
     6961 ccgagtcagt ttttattttt ctactaattt ggtcgtttat ttcggcgtgt aggacatggc
     7021 aaccgggcct gaatttcgcg ggtattctgt ttctattcca actttttctt gatccgcagc
     7081 cattaacgac ttttgaatag atacgctgac acgccaagcc tcgctagtca aaagtgtacc
     7141 aaacaacgct ttacagcaag aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc
     7201 ttttcagaaa tggataaata gccttgcttc ctattatatc ttcccaaatt accaatacat
     7261 tacactagca tctgaatttc ataaccaatc tcgatacacc aaatcgccat ggcttctatg
     7321 atatcctctt cagctgtgac tacagtcagc cgtgcttcta cggtgcaatc ggccgcggtg
     7381 gctccattcg gcggcctcaa atccatgact ggattcccag ttaagaaggt caacactgac
     7441 attacttcca ttacaagcaa tggtggaaga gtaaagtgca tggtgacggt gttcggcatt
     7501 ctgaatctca ccgaggactc cttcttcgat gagagccggc ggctagaccc cgccggcgct
     7561 gtcaccgcgg cgatcgaaat gctgcgagtc ggatcagacg tcgtggatgt cggaccggcc
     7621 gccagccatc cggacgcgag gcctgtatcg ccggccgatg agatcagacg tattgcgccg
     7681 ctcttagacg ccctgtccga tcagatgcac cgtgtttcaa tcgacagctt ccaaccggaa
     7741 acccagcgct atgcgctcaa gcgcggcgtg ggctacctga acgatatcca aggatttcct
     7801 gaccctgcgc tctatcccga tattgctgag gcggactgca ggctggtggt tatgcactca
     7861 gcgcagcggg atggcatcgc cacccgcacc ggtcaccttc gacccgaaga cgcgctcgac
     7921 gagattgtgc ggttcttcga ggcgcgggtt tccgccttgc gacggagcgg ggtcgctgcc
     7981 gaccggctca tcctcgatcc ggggatggga tttttcttga gccccgcacc ggaaacatcg
     8041 ctgcacgtgc tgtcgaacct tcaaaagctg aagtcggcgt tggggcttcc gctacttgtc
     8101 tcggtgtcgc ggaaatcctt cttgggcgcc accgttggcc ttcctgtaaa ggatctgggt
     8161 ccagcgagcc ttgcggcgga acttcacgcg atcggcaatg gcgctgacta cgtccgcacc
     8221 cacgcgcctg gagatctgcg aagcgcaatc accatctcgg aaaccctcgc gaaatttcgc
     8281 agtcgcgacg ccagagacag agggttagat catgcctagc ttggctgcag gtcgacctgc
     8341 aggcatgccc gctgaaatca ccagtctctc tctacaaatc tatctctctc tataataatg
     8401 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     8461 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     8521 taattcctaa aaccaaaatc caggggtacc gtattctaga ccaagcatgc acagctggcg
     8581 ttctcagcca gatccccttg acaggatata ttggcgggta aactaagtcg cyrtatgtgt
     8641 ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9121 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9181 tgaagccagt taccttcgga araagagttg gtagctcttg atccggcaaa caaaccaccg
     9241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9421 aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattatttgc
     9481 cgactacctt ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc
     9541 gcgaggccaa gcgatcttct tgtccaagat aagcctgcct agcttcaagt atgacgggct
     9601 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     9661 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     9721 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     9781 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     9841 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     9901 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     9961 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
    10021 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
    10081 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
    10141 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
    10201 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
    10261 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
    10321 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
    10381 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
    10441 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca
    10501 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac
    10561 agcccgccgt cgagcgggct
//
