LOCUS       sequence-227498-       12899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 12899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW725 - pUFO>>
FEATURES             Location/Qualifiers
     source          1..12899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             6333..6677
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      6684..7325
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     protein_bind    7431..7450
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7483..7502
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7535..7554
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7587..7606
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7639..7658
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7691..7710
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        7734..7780
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(7865..7867,7868..7870,7871..8581)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7910..7931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8171..8190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8518..8539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9245..9625
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    10918..10942
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(11033..11621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11113..11132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11792..12580)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(12871..12899,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta
      601 attaaacacg gcgcgccaac agcgatcgca ccaggtacca cctgatatcg aattctctgt
      661 tttaattgcc ccacttctac aattgaacaa cacctccccc aaaaaaaaac tatcgctcat
      721 ctccacggtt ttaatttttt tctgcaacta tgatatccaa ttttcctcga ccatacataa
      781 ttataatctt tgctgatata ccaaaatttt agaatttttt tattaaatac agtttacagc
      841 gatgaagaca ttaattttgg catttgtatt ttgcgggaga atacaaacaa caaggtcagt
      901 catctacttc acatgcattc aaatttatat tcttttatgt tagacaatgt tataagcctt
      961 tgtttcttaa ttcgtaatat ggtgttattt tccaaatcta taatttggct tattagtttg
     1021 cagttgaaca agctagggca tgtatagatg tgtctttata gttattagcg tatacaaaga
     1081 acaaattgga cgatagatgt gtctttcaat aatttcttat tttttcctta caatgttttt
     1141 ttttgtcttt ctgtatttga tgttttcttc ataataattg cattttctat ccctctcgaa
     1201 aattagtacc tcaatcaaat tgatctcaag tcaagttctg gacacaacta ccttttatct
     1261 ttttgaattt aatgttaaat tatattcaaa ataaaaataa acgtttttct ttgaatttaa
     1321 ctctatttgt tttcttttgg aaagttgatg agtgattgtc ggtaactata ctaaaaatgg
     1381 ttttgcattt ttctataggt gtggtatgcc ttggtacgtg gaggaaacaa gttagacaca
     1441 taagcacata tgtactagac gcaaataatg tcacaaaaac ggtttaaaat atctggtttt
     1501 cagtttgcat gtcgtactta taaccgaacg tgtgtaaagt tctcatgtca tataccagtt
     1561 ctgttaaact aatgaaatat gttcagaggt ttaaccacta taactattag acccgatttc
     1621 aattaatcaa gaaatatcca aactttttaa tatatgcatt atgctttaca aaattgtttg
     1681 atcttctaat gatgtaaaga catttacaaa atataattat ttaattgatt ctaactaatt
     1741 tgtccgcgca taacgctggc cgtaaactag ttcttcttac aaattacaat atcaatttcc
     1801 actttgggcc ggaaatttat atccataact ctactacgta ccaattaaca catgtgtgta
     1861 aataaaacta cataatggca tgaatggagt ttcgaatcag ttaatgtggg cgacaaaata
     1921 aaatcagatt atgttttatt attaagttta gaaagagatg cttcattaaa agtttatgct
     1981 ttaatgttaa atcagactat ttattatggg tacgacttgt aagcgtaatt caaagttaaa
     2041 caaaacctag ttgggaaatg ggaagataga tgatttgata aaaacaagac actgtttggt
     2101 ttcagagact tcctttagca tcaaaacaca aacaaaagcg aagcctcttg agttactgca
     2161 aatagagaat attattaccc ttttgcgact tgtcagcttc agatattctc acttgtatta
     2221 ttattttcac ggtaaacaat gccttaaata agaaaccctg attggacttt tgatctgact
     2281 ctactcttca ctctttcttc ttctttattt tcagtcatga tgtctctcta accctaatct
     2341 caaaaaatcc aaactccttt tatttatttc taaaccttga ttatagctag caatgattaa
     2401 tataagaatt ttttttctgg ataaagaatt aattagaaat tgagttgtaa atgttttgtg
     2461 atgtctaaaa ttcttttgtt tgcaaattag atgtaaattg atatatttga gatttgtatg
     2521 aaagctagtt ttattttccc taaacaagaa gttcattatc ttggacttgg aggttttaga
     2581 gtttgaaaga gtttacaatt tataagaaaa aataatcact atatatatat atatagtgta
     2641 tataatgaat tgtttcacat taaattgcaa caacatcaaa taagggtaac atactaacat
     2701 atagtttgtt tgtttactct ttaaaaaaag gggataaact aagagctatt tctgctataa
     2761 tttaggaaca aactggatca catgacaaaa atgcatcata attcatatta aattttgtgt
     2821 atatctattt ctcatgttta gaaataacat tcttgtgtgt tatacatgtt atcagttttt
     2881 cttcctagat ggaagtttta ttgttggagt cttttaaaac catactcact atgttcctct
     2941 ttatttgatg ttttggattt tagatagaaa ttaataaaaa atatgttttc tatttttata
     3001 aaattatttt tttgttagtt tagtaatttt atttttcttt ttttattatt agtcacaagc
     3061 aaaaataata acaatatttt tataaaacat taattttggt cgaacaagta aaaataactc
     3121 aaaacatcaa ataattagaa actaaaaaag tagatattgt caaattttgt gttgagttcg
     3181 aataagataa tgtgctctcc tccaacaaaa ttatttagaa taaatgcact tctatgacat
     3241 tagagaacca actaatttat ttagaataaa acacacatat atattaacat ataaagtaat
     3301 tctaattggc ttgatataat atataagtaa aaaaatgatc ttaataatct ctagttttct
     3361 tgggttgatc tccacgagta caatttgact gaccattata gaagttgaga agcgtgcatg
     3421 taataaaagt tgtatattac aaattagaga ggaaaaagaa agaaagaaaa aagatttgaa
     3481 gatgtgatca agtgtgaaaa gtattggagt agtctccaaa ttaataattt cgatgctggg
     3541 cattgacaag ataactctga agctctcaac tttaagacca tcacttcctc tgccaccatt
     3601 ttcacgttta cccaaacaca cacatataca aacaaaattt tgttagtcaa taattatcac
     3661 caaactgggg ttataacaag gcttttggat acttgtgctt gttgatgttc taggttcgta
     3721 tgataacaaa gtacatccgt tatatatatt cgaaacacac tttaatatta aaaatatata
     3781 tccaattttc ttgtgaaatt tagattattt ggaattaaac ctatttctct tgtcttggcc
     3841 acttgaccgg tttagttttt tagacgtatt ttattatttc tgtttagaaa ataacaacat
     3901 atatcaactg attttttact tccaatctct ttttgtcagc acacaaatag aaaaacgtct
     3961 gtaagctaag ctatcaacta aaacattaac atatataatc ttttacgttg atagaaaata
     4021 aacataaatt tctgagttat tttttttttg gttggtgtgt cactacttac ttactactat
     4081 acctttttaa caataaagaa acactatttc tttttctatt caatataata tatgttttct
     4141 atttgtataa atccattacc tttgtttgtt ttataccaaa tgttctttat atataagtat
     4201 atgccacctt actctattga agtcaagaac atatcaaaaa cccatgcaag aagctcacag
     4261 agaaagacca aacacttttg tctctttctt caaaactttt acatatgatc tttgcctctt
     4321 ttcctacaat gggttttgca taactttcac caaaaccctc ctcaaaagcc cttcacatat
     4381 tcccaacaca agaaaataaa ctctaaatcc actttcacca aatcttttca tttttcagct
     4441 aaaataacac aatggctagt gaagctcgaa aaacaaagaa aaaaatcaaa gggattcagc
     4501 aagccactgc aggagtctca caagacactt cggaaaatcc taacaaaaca atagttcctg
     4561 cagcattacc acagctcacc cctaccttgg tgtcactgct ggaggtgatt gaacccgagg
     4621 tgttgtatgc aggatatgat agctctgttc cagattcagc atggagaatt atgaccacac
     4681 tcaacatgtt aggtgggcgt caagtgattg cagcagtgaa atgggcaaag gcgataccag
     4741 gcttcagaaa cttacacctg gatgaccaaa tgaccctgct acagtactca tggatgtttc
     4801 tcatggcatt tgccctgggt tggagatcat acagacaatc aagtggaaac ctgctctgct
     4861 ttgctcctga tctgattatt aatgagcaga gaatgtctct accctgcatg tatgaccaat
     4921 gtaaacacat gctgtttgtc tcctctgaat tacaaagatt gcaggtatcc tatgaagagt
     4981 atctctgtat gaaaacctta ctgcttctct cctcagttcc taaggaaggt ctgaagagcc
     5041 aagagttatt tgatgagatt cgaatgactt atatcaaaga gctaggaaaa gccatcgtca
     5101 aaagggaagg gaactccagt cagaactggc aacggtttta ccaactgaca aagcttctgg
     5161 actccatgca tgaggtggtt gagaatctcc ttacctactg cttccagaca tttttggata
     5221 agaccatgag tattgaattc ccagagatgt tagctgaaat catcactaat cagataccaa
     5281 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatctact agcaaaccgg
     5341 taacgttata cgacgtcgct gaatacgccg gcgtttctca tcaaaccgtt tctagagtgg
     5401 ttaaccaggc ttcacatgtt agcgctaaaa cccgggaaaa agttgaagct gccatggctg
     5461 agctcaacta catcccgaac cgtgttgcgc agcagctggc tggtaaacaa agcttgctga
     5521 tcggtgtcgc gacctcgagc ttggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     5581 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     5641 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     5701 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     5761 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     5821 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     5881 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     5941 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     6001 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     6061 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     6121 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     6181 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     6241 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     6301 tgaaaagaaa aaccactagt ggatcggaat tcgccaattt taatcaaagt gggaatattg
     6361 ctgatagctc attgtccttc actttcacta acagtagcaa cggtccgaac ctcataacaa
     6421 ctcaaacaaa ttctcaagcg ctttcacaac caattgcctc ctctaacgtt catgataact
     6481 tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg taataattca aaaccactgt
     6541 cacctggttg gacggaccaa actgcgtata acgcgtttgg aatcactaca gggatgttta
     6601 ataccactac aatggatgat gtatataact atctattcga tgatgaagat accccaccaa
     6661 acccaaaaaa agagtaactg caaagctttc gttcgtatca tcggtttcga caacgttcgt
     6721 caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt ttgagtatta
     6781 tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt
     6841 ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg
     6901 atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg
     6961 tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa
     7021 atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag ttgtaccatt
     7081 atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca aatgttactg
     7141 aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat gtaattttcc
     7201 agaatccttg tcagattcta atcattgctt tataattata gttatactca tggatttgta
     7261 gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt gacaacatgc
     7321 atcaatcgac tatgagtagg caagatgttc tgtaggggct atgatgcctg gtcacaacct
     7381 atgcatatgt cgagctcaag aaatctagaa agaagaaagg gaagagaaag aattgtgagc
     7441 gctcacaatt gaaagactag aaagaagaaa gggaagagaa agaattgtga gcgctcacaa
     7501 ttgaaagact agaaagaaga aagggaagag aaagaattgt gagcgctcac aattgaaaga
     7561 ctagaaagaa gaaagggaag agaaagaatt gtgagcgctc acaattgaaa gactagaaag
     7621 aagaaaggga agagaaagaa ttgtgagcgc tcacaattga aagactagaa agaagaaagg
     7681 gaagagaaag aattgtgagc gctcacaatt gaaagactag tggatcgatc ttcgcaagac
     7741 ccttcctcta tataaggaag ttcatttcat ttggagagga cgaacaatga aagccttcac
     7801 actcgctctc ttcttagctc tttccctcta tctcctgccc aatccagcca ctagtggctc
     7861 aacaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     7921 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     7981 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     8041 caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
     8101 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     8161 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     8221 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     8281 gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg acaagcagaa
     8341 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     8401 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     8461 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     8521 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     8581 gtcaggtcat gatgagcttt agctgcatct cgtctctgtt atgcttaaga agttcaatgt
     8641 ttcgtttcat gtaaaacttt ggtggtttgt gttttggggc cttgtataat ccctgatgaa
     8701 taagtgttct actatgtttc cgttcctgtt atctctttct ttctaatgac aagtcgaact
     8761 tcttctttat catcgcttcg tttttattat ctgtgcttct tttgtttaat acgcctgcaa
     8821 agtgactcga ctctgtttag tgcagttctg cgaaacttgt aaatagtcca attgttggcc
     8881 tctagtaata gatgtagcga aagtgttgag ctgttgggtt ctaaggatgg cttgaacatg
     8941 ttaatctttt aggttctgag tatgatgaac attcgttgtt gctaagaaat gcctgtaatg
     9001 tcccacaaat gtagaaaatg gttcgtacct ttgtccaagc attgatatgt ctgatgagag
     9061 gaaactgcaa gatactgagc ttggtttaac gaaggagagg cagtttcttc cttccaaagc
     9121 atttcatttg acaatgcctt gatcatctta agtagagttt ctgttgtgga aagtttgaaa
     9181 ctttgaagaa acgactctca agtaaattga tgatcacaag tgaaagtgta tgttactagg
     9241 atccttttca aatcagtgcg caagacgtga cgtaagtatc cgagtcagtt tttatttttc
     9301 tactaatttg gtcgtttatt tcggcgtgta ggacatggca accgggcctg aatttcgcgg
     9361 gtattctgtt tctattccaa ctttttcttg atccgcagcc attaacgact tttgaataga
     9421 tacgctgaca cgccaagcct cgctagtcaa aagtgtacca aacaacgctt tacagcaaga
     9481 acggaatgcg cgtgacgctc gcggtgacgc catttcgcct tttcagaaat ggataaatag
     9541 ccttgcttcc tattatatct tcccaaatta ccaatacatt acactagcat ctgaatttca
     9601 taaccaatct cgatacacca aatcgccatg gcttctatga tatcctcttc agctgtgact
     9661 acagtcagcc gtgcttctac ggtgcaatcg gccgcggtgg ctccattcgg cggcctcaaa
     9721 tccatgactg gattcccagt taagaaggtc aacactgaca ttacttccat tacaagcaat
     9781 ggtggaagag taaagtgcat ggtgacggtg ttcggcattc tgaatctcac cgaggactcc
     9841 ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc gatcgaaatg
     9901 ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg
     9961 cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc cctgtccgat
    10021 cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta tgcgctcaag
    10081 cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct ctatcccgat
    10141 attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga tggcatcgcc
    10201 acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg gttcttcgag
    10261 gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat cctcgatccg
    10321 gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct gtcgaacctt
    10381 caaaagctga agtcggcgtt ggggcttccg ctacttgtct cggtgtcgcg gaaatccttc
    10441 ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct tgcggcggaa
    10501 cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg agatctgcga
    10561 agcgcaatca ccatctcgga aaccctcgcg aaatttcgca gtcgcgacgc cagagacaga
    10621 gggttagatc atgcctagct tggctgcagg tcgacctgca ggcatgcccg ctgaaatcac
    10681 cagtctctct ctacaaatct atctctctct ataataatgt gtgagtagtt cccagataag
    10741 ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
    10801 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
    10861 aggggtaccg tattctagac caagcatgca cagctggcgt tctcagccag atccccttga
    10921 caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag
    10981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    11041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    11101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11521 gaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11761 aaagtatata tgtgtaacat tggtctagtg attatttgcc gactaccttg gtgatctcgc
    11821 ctttcacgta gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
    11881 gtccaagata agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
    11941 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
    12001 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
    12061 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
    12121 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
    12181 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
    12241 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
    12301 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
    12361 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
    12421 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
    12481 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
    12541 atagttgagt cgatacttcg gcgatcaccg cttccctcat aacacccctt gtattactgt
    12601 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
    12661 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
    12721 aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca
    12781 aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct
    12841 ggctggatga tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggct
//
