LOCUS       sequence-227515-        4296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry clone for SLC7A11 insensitive to hairpin TRCN0000288926 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, 
            Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, 
            Cravatt BF, Clardy J, Kaelin WG Jr
  TITLE     Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 
            Senses Cysteine.
  JOURNAL   Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042.
  PUBMED    27368101
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-30- 
            30"; volume: "166"; issue: "1"; pages: "126-39"
COMMENT     Sequence Label: pDONR223-SLC7A11 sh926R
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(116..202)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     251..273
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     265..282
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     266..282
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    298..397
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1904..2003)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2020..2039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2021..2039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2044..2060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2044..2060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2491..3282
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3375..3963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3864..3883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4117..4134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(join(4293..4296,1..24))
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
       61 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
      121 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
      181 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
      241 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
      301 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
      361 ttttttataa tgccaacttt gtacaaaaaa gcaggcttca tggtcagaaa gcctgttgtg
      421 tccaccatct ccaaaggagg ttacctgcag ggaaatgtta acgggaggct gccttccctg
      481 ggcaacaagg agccacctgg gcaggagaaa gtgcagctga agaggaaagt cactttactg
      541 aggggagtct ccattatcat tggcaccatc attggagcag gaatcttcat ctctcctaag
      601 ggcgtgctcc agaacacggg cagcgtgggc atgtctctga ccatctggac ggtgtgtggg
      661 gtcctgtcac tatttggagc tttgtcttat gctgaattgg gaacaactat aaagaaatct
      721 ggaggtcatt acacatatat tttggaagtc tttggtccat taccagcttt tgtacgagtc
      781 tgggtggaac tcctcataat acgccctgca gctactgctg tgatatccct ggcatttgga
      841 cgctacattc tggaaccatt ttttattcaa tgtgaaatcc ctgaacttgc gatcaagctc
      901 attacagctg tgggcataac tgtagtgatg gtcctaaata gcatgagtgt cagctggagc
      961 gcccggatcc agattttctt aaccttttgc aagctcacag caattctgat aattatagtg
     1021 cccggcgtga tgcaattgat taaaggtcaa acgcagaact ttaaagacgc cttttcagga
     1081 agagattcaa gtattacgcg gttgccactg gctttttatt atggaatgta tgcatatgct
     1141 ggctggtttt acctcaactt tgttactgaa gaagtagaaa accctgaaaa aaccattccc
     1201 cttgcaatat gtatatccat ggccattgtc accattggct atgtgctgac aaatgtggcc
     1261 tactttacga ccattaatgc tgaggagctg ctgctttcaa atgcagtggc agtgaccttt
     1321 tctgagcggc tactgggaaa tttctcatta gcagttccga tctttgttgc cctctcctgc
     1381 tttggctcca tgaacggtgg tgtgtttgct gtctccaggt tattctatgt tgcgtctcga
     1441 gagggtcacc ttccagaaat cctctccatg attcatgtcc gcaagcacac tcctctacca
     1501 gctgttattg ttttgcaccc tttgacaatg ataatgctct tctctggaga cctcgacagt
     1561 cttttgaatt tcctcagttt tgccaggtgg ctttttattg ggctggcagt tgctgggctg
     1621 atttatcttc gatacaaatg cccagatatg catcgtcctt tcaaggtgcc actgttcatc
     1681 ccagctttgt tttccttcac atgcctcttc atggttgccc tttccctcta ttcggaccca
     1741 tttagtacag ggattggctt cgtcatcact ctgactggag tccctgcgta ttatctcttt
     1801 attatatggg acaagaaacc caggtggttt agaataatgt cagagaaaat aaccagaaca
     1861 ttacaaataa tactggaagt tgtaccagaa gaagataagt taaacccagc tttcttgtac
     1921 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1981 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     2041 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2101 gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg
     2161 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     2221 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     2281 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2341 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     2401 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     2461 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
     2521 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     2581 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     2641 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     2701 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     2761 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     2821 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     2881 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     2941 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     3001 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     3061 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     3121 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     3181 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     3241 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     3301 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4081 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4141 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4201 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4261 accgctagcc aggaagagtt tgtagaaacg caaaaa
//
