LOCUS       sequence-227522-        8814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of shRNA1 against human HIF1a 
            (Dox-inducible).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, 
            Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, 
            Cravatt BF, Clardy J, Kaelin WG Jr
  TITLE     Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 
            Senses Cysteine.
  JOURNAL   Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042.
  PUBMED    27368101
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-30- 
            30"; volume: "166"; issue: "1"; pages: "126-39"
COMMENT     Sequence Label: pLKO.1-TetON-Puro-HIF1a 3809
FEATURES             Location/Qualifiers
     source          1..8814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        287..381
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     298..317
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    335..353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    480..597
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        646..1156
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1075..1093
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          1165..1737
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1745..1764
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1792..1810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1821..2441
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1894..1913)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2476..3049
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2643..2660)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2870..2889
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3069..3668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3069..3088)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3565..3585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3796..4029
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4101..4222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4138..4157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4192..4211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4262..4397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4418..4436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4446..4463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4446..4462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4455..4477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4604..5059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4691..4710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4901..4922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5085..5189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5190..5258,5259..6050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5408..5427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6221..6809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6710..6729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6963..6980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7097..7118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7133..7138,7139..7156,7157..7163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7171..7187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7176..7198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7195..7211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7195..7211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7230..7250
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7232..7250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7278..7504
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7505..7685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7732..7857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8350..8583
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8768..8812
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
       61 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
      121 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
      181 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
      241 cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgcaatat ttgcatgtcg
      301 ctatgtgttc tgggaaatca ccataaacgt gaaatcccta tcagtgatag agacttataa
      361 gttccctatc agtgatagag acaccggcag ttatgattgt gaagttactc gagtaacttc
      421 acaatcataa ctggttttta attctcgacc tcgagacaaa tggcagtatt catccacaat
      481 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      541 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      601 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
      661 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
      721 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
      781 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
      841 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
      901 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
      961 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     1021 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     1081 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     1141 cgacctctct ccccaggggg atctgtgagt ttggggaccc ttgattgttc tttctttttc
     1201 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
     1261 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
     1321 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
     1381 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
     1441 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
     1501 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
     1561 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc
     1621 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
     1681 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1741 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gtaatacgac
     1801 tcactatagg gcgaattgat atgtctagat tagataaaag taaagtgatt aacagcgcat
     1861 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     1921 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     1981 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     2041 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     2101 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     2161 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     2221 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     2281 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     2341 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     2401 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg
     2461 agttctaggg atctgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     2521 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     2581 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     2641 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     2701 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     2761 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     2821 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     2881 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     2941 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     3001 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg
     3061 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     3121 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     3181 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     3241 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     3301 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     3361 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     3421 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     3481 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     3541 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     3601 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     3661 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     3721 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3781 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3841 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3901 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3961 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4021 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4081 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4201 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4261 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4321 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4381 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4441 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4801 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4921 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4981 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5041 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5101 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5401 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5701 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6901 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6961 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7021 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7081 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7141 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7201 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7261 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7321 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7381 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7441 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7501 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7561 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7621 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7681 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7741 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7801 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7861 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7921 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7981 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8041 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8101 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8161 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8221 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8281 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8341 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8401 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8461 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8521 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8581 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8641 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8701 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8761 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtg
//
