LOCUS       sequence-227523-        4608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry clone for NRF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, 
            Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, 
            Cravatt BF, Clardy J, Kaelin WG Jr
  TITLE     Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 
            Senses Cysteine.
  JOURNAL   Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042.
  PUBMED    27368101
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-30- 
            30"; volume: "166"; issue: "1"; pages: "126-39"
COMMENT     Sequence Label: pDONR223 NRF2
FEATURES             Location/Qualifiers
     source          1..4608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(146..173)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(265..351)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     400..422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     414..431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     415..431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    447..546
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2365..2464)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2481..2500)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2482..2500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2505..2521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2505..2521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2952..3743
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3836..4424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4325..4344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4578..4595
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
       61 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
      121 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
      181 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
      241 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
      301 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      361 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
      421 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
      481 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
      541 caggcttcat gatggacttg gagctgccgc cgccgggact cccgtcccag caggacatgg
      601 atttgattga catactttgg aggcaagata tagatcttgg agtaagtcga gaagtatttg
      661 acttcagtca gcgacggaaa gagtatgagc tggaaaaaca gaaaaaactt gaaaaggaaa
      721 gacaagaaca actccaaaag gagcaagaga aagccttttt cgctcagtta caactagatg
      781 aagagacagg tgaatttctc ccaattcagc cagcccagca catccagtca gaaaccagtg
      841 gatctgccaa ctactcccag gttgcccaca ttcccaaatc agatgctttg tactttgatg
      901 actgcatgca gcttttggcg cagacattcc cgtttgtaga tgacaatgag gtttcttcgg
      961 ctacgtttca gtcacttgtt cctgatattc ccggtcacat cgagagccca gtcttcattg
     1021 ctactaatca ggctcagtca cctgaaactt ctgttgctca ggtagcccct gttgatttag
     1081 acggtatgca acaggacatt gagcaagttt gggaggagct attatccatt cctgagttac
     1141 agtgtcttaa tattgaaaat gacaagctgg ttgagactac catggttcca agtccagaag
     1201 ccaaactgac agaagttgac aattatcatt tttactcatc tataccctca atggaaaaag
     1261 aagtaggtaa ctgtagtcca cattttctta atgcttttga ggattccttc agcagcatcc
     1321 tctccacaga agaccccaac cagttgacag tgaactcatt aaattcagat gccacagtca
     1381 acacagattt tggtgatgaa ttttattctg ctttcatagc tgagcccagt atcagcaaca
     1441 gcatgccctc acctgctact ttaagccatt cactctctga acttctaaat gggcccattg
     1501 atgtttctga tctatcactt tgcaaagctt tcaaccaaaa ccaccctgaa agcacagcag
     1561 aattcaatga ttctgactcc ggcatttcac taaacacaag tcccagtgtg gcatcaccag
     1621 aacactcagt ggaatcttcc agctatggag acacactact tggcctcagt gattctgaag
     1681 tggaagagct agatagtgcc cctggaagtg tcaaacagaa tggtcctaaa acaccagtac
     1741 attcttctgg ggatatggta caacccttgt caccatctca ggggcagagc actcacgtgc
     1801 atgatgccca atgtgagaac acaccagaga aagaattgcc tgtaagtcct ggtcatcgga
     1861 aaaccccatt cacaaaagac aaacattcaa gccgcttgga ggctcatctc acaagagatg
     1921 aacttagggc aaaagctctc catatcccat tccctgtaga aaaaatcatt aacctccctg
     1981 ttgttgactt caacgaaatg atgtccaaag agcagttcaa tgaagctcaa cttgcattaa
     2041 ttcgggatat acgtaggagg ggtaagaata aagtggctgc tcagaattgc agaaaaagaa
     2101 aactggaaaa tatagtagaa ctagagcaag atttagatca tttgaaagat gaaaaagaaa
     2161 aattgctcaa agaaaaagga gaaaatgaca aaagccttca cctactgaaa aaacaactca
     2221 gcaccttata tctcgaagtt ttcagcatgc tacgtgatga agatggaaaa ccttattctc
     2281 ctagtgaata ctccctgcag caaacaagag atggcaatgt tttccttgtt cccaaaagta
     2341 agaagccaga tgttaagaaa aacaacccag ctttcttgta caaagttggc attataagaa
     2401 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2461 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2521 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2581 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2641 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2701 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2761 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2821 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2881 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2941 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3001 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3061 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3121 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     3181 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3241 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3301 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3361 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3421 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3481 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3541 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3601 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3661 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3721 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3781 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3841 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3901 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3961 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4381 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4441 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4501 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4561 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagc
//
