LOCUS       sequence-22753-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Soh JW, Weinstein IB
  TITLE     Roles of specific isoforms of protein kinase C in the 
            transcriptional control of cyclin D1 and related genes.
  JOURNAL   J Biol Chem. 2003 Sep 5. 278(36):34709-16.
  PUBMED    12794082
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 5. 278(36):34709-16."
COMMENT     Sequence Label: PKC beta II CAT
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cnggggncat tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agcttggtac cgagctcgga tcgaattctc
      121 gaggccacca tggcttatcc ttacgacgtg cctgactacg ccggatccga caacaatggc
      181 aacagggacc ggatgaaact gaccgatttt aacttcctga tggtgctggg gaaaggcagc
      241 tttggcaagg tcatgctctc agagcggaag ggtacagatg aactctatgc cgtgaagatc
      301 ctgaagaagg acgtggtgat ccaagatgac gatgtggagt gcaccatggt ggagaagcgg
      361 gtgctggccc tgcctgggaa gcccccgttc ctgactcagc tccattcctg cttccagacc
      421 atggaccgcc tgtactttgt gatggagtat gtgaacgggg gtgacctcat gtaccacatc
      481 caacaagttg gccgtttcaa ggagccccat gctgtatttt acgccgcaga gattgccatc
      541 ggtctgttct tcttgcagag caagggcatt atttaccgcg acctgaaact tgacaacgtg
      601 atgctggatt ctgaggggca catcaaaatc gctgactttg gcatgtgtaa ggagaacatc
      661 tgggatgggg tgacaaccaa gacattctgt ggcactccgg actacattgc tccagagatc
      721 attgcttatc agccnacggg agtctgtgga ctggtgggca tttgantctg ctgtatga
//
