LOCUS       sequence-227530-       14706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 14706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL41 - pSMXL5>>
FEATURES             Location/Qualifiers
     source          1..14706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        complement(1850..2230)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             complement(join(2894..3604,3605..3607,3608..3610))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2936..2957)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3285..3304
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3544..3565
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3695..3741)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    3765..3784
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3817..3836
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3869..3888
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3921..3940
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             complement(4526..4870)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    12725..12749
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(12840..13428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12920..12939)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13599..14387)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(14678..14706,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ggatctggct gagaacgcca gctgtgcatg cttggtctag
      601 aatacggtac ccctggattt tggttttagg aattagaaat tttattgata gaagtatttt
      661 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
      721 agaaccctaa ttcccttatc tgggaactac tcacacatta ttatagagag agatagattt
      781 gtagagagag actggtgatt tcagcgggca tgcctgcagg tcgacctgca gccaagctag
      841 gcatgatcta accctctgtc tctggcgtcg cgactgcgaa atttcgcgag ggtttccgag
      901 atggtgattg cgcttcgcag atctccaggc gcgtgggtgc ggacgtagtc agcgccattg
      961 ccgatcgcgt gaagttccgc cgcaaggctc gctggaccca gatcctttac aggaaggcca
     1021 acggtggcgc ccaagaagga tttccgcgac accgagacaa gtagcggaag ccccaacgcc
     1081 gacttcagct tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc ggggctcaag
     1141 aaaaatccca tccccggatc gaggatgagc cggtcggcag cgaccccgct ccgtcgcaag
     1201 gcggaaaccc gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc gggtcgaagg
     1261 tgaccggtgc gggtggcgat gccatcccgc tgcgctgagt gcataaccac cagcctgcag
     1321 tccgcctcag caatatcggg atagagcgca gggtcaggaa atccttggat atcgttcagg
     1381 tagcccacgc cgcgcttgag cgcatagcgc tgggtttccg gttggaagct gtcgattgaa
     1441 acacggtgca tctgatcgga cagggcgtct aagagcggcg caatacgtct gatctcatcg
     1501 gccggcgata caggcctcgc gtccggatgg ctggcggccg gtccgacatc cacgacgtct
     1561 gatccgactc gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc tagccgccgg
     1621 ctctcatcga agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt caccatgcac
     1681 tttactcttc caccattgct tgtaatggaa gtaatgtcag tgttgacctt cttaactggg
     1741 aatccagtca tggatttgag gccgccgaat ggagccaccg cggccgattg caccgtagaa
     1801 gcacggctga ctgtagtcac agctgaagag gatatcatag aagccatggc gatttggtgt
     1861 atcgagattg gttatgaaat tcagatgcta gtgtaatgta ttggtaattt gggaagatat
     1921 aataggaagc aaggctattt atccatttct gaaaaggcga aatggcgtca ccgcgagcgt
     1981 cacgcgcatt ccgttcttgc tgtaaagcgt tgtttggtac acttttgact agcgaggctt
     2041 ggcgtgtcag cgtatctatt caaaagtcgt taatggctgc ggatcaagaa aaagttggaa
     2101 tagaaacaga atacccgcga aattcaggcc cggttgccat gtcctacacg ccgaaataaa
     2161 cgaccaaatt agtagaaaaa taaaaactga ctcggatact tacgtcacgt cttgcgcact
     2221 gatttgaaaa ggatcctagt aacatacact ttcacttgtg atcatcaatt tacttgagag
     2281 tcgtttcttc aaagtttcaa actttccaca acagaaactc tacttaagat gatcaaggca
     2341 ttgtcaaatg aaatgctttg gaaggaagaa actgcctctc cttcgttaaa ccaagctcag
     2401 tatcttgcag tttcctctca tcagacatat caatgcttgg acaaaggtac gaaccatttt
     2461 ctacatttgt gggacattac aggcatttct tagcaacaac gaatgttcat catactcaga
     2521 acctaaaaga ttaacatgtt caagccatcc ttagaaccca acagctcaac actttcgcta
     2581 catctattac tagaggccaa caattggact atttacaagt ttcgcagaac tgcactaaac
     2641 agagtcgagt cactttgcag gcgtattaaa caaaagaagc acagataata aaaacgaagc
     2701 gatgataaag aagaagttcg acttgtcatt agaaagaaag agataacagg aacggaaaca
     2761 tagtagaaca cttattcatc agggattata caaggcccca aaacacaaac caccaaagtt
     2821 ttacatgaaa cgaaacattg aacttcttaa gcataacaga gacgagatgc agctaaagct
     2881 catcatgacc tgacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     2941 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     3001 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     3061 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
     3121 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgcta aagtagttgt
     3181 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     3241 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     3301 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     3361 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg
     3421 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     3481 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     3541 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     3601 tgctcaccat tgttgagcca ctagtggctg gattgggcag gagatagagg gaaagagcta
     3661 agaagagagc gagtgtgaag gctttcattg ttcgtcctct ccaaatgaaa tgaacttcct
     3721 tatatagagg aagggtcttg cgaagatcga tccactagtc tttcaattgt gagcgctcac
     3781 aattctttct cttccctttc ttctttctag tctttcaatt gtgagcgctc acaattcttt
     3841 ctcttccctt tcttctttct agtctttcaa ttgtgagcgc tcacaattct ttctcttccc
     3901 tttcttcttt ctagtctttc aattgtgagc gctcacaatt ctttctcttc cctttcttct
     3961 ttctagattt cttgagctcg acatatgcat aggttgtgac caggcatcat agcccctaca
     4021 gaacatcttg cctactcata gttcttgtta actttccaaa atttgatttc aagttcgtta
     4081 tcgcgtttgc aatcgcatac tcgaaaatag cgttgcgttt ggtagattct atttcgtgtt
     4141 tatggcggtt ttaagcctat tgggcttttt ggcttcatac tcagcccaat atactattgc
     4201 tttttttaac caatttgtta cttttttttt tttttcgtaa ccaatctttg ttacgttgaa
     4261 acttcttttg taccattaac atctatgcaa atgttatttt gttccattac catagtcatt
     4321 aacagcatcc atgcaagatg ttatttttct tacataatcc taataaaaat attttcccaa
     4381 atttaaaacg catttatatt acattcgaag aagaaaagag ttacaccagt caaaccccca
     4441 cattagtaat ggaaatttta catattgaaa aagaaagaac gaaagaaaaa gaaacaaaag
     4501 aaatttaacc gaaagattct agcagttact ctttttttgg gtttggtggg gtatcttcat
     4561 catcgaatag atagttatat acatcatcca ttgtagtggt attaaacatc cctgtagtga
     4621 ttccaaacgc gttatacgca gtttggtccg tccaaccagg tgacagtggt tttgaattat
     4681 taccatcatc aattttacta gccgtgattt cattattcat gaagttatca tgaacgttag
     4741 aggaggcaat tggttgtgaa agcgcttgag aatttgtttg agttgttatg aggttcggac
     4801 cgttgctact gttagtgaaa gtgaaggaca atgagctatc agcaatattc ccactttgat
     4861 taaaattggc gaattccgat ccactagtgg tttttctttt caccagtgag acgggcaaca
     4921 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     4981 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     5041 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     5101 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     5161 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     5221 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     5281 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     5341 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     5401 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     5461 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     5521 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
     5581 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
     5641 gcgacggcgc gtgcagggcc aagctcgagg tcgcgacacc gatcagcaag ctttgtttac
     5701 cagccagctg ctgcgcaaca cggttcggga tgtagttgag ctcagccatg gcagcttcaa
     5761 ctttttcccg ggttttagcg ctaacatgtg aagcctggtt aaccactcta gaaacggttt
     5821 gatgagaaac gccggcgtat tcagcgacgt cgtataacgt taccggtttg ctagtagatt
     5881 tttgatgaaa cagaagcttt ttgatatttc catttgaata ttttggtatc tgattagtga
     5941 tgatttcagc taacatctct gggaattcaa tactcatggt cttatccaaa aatgtctgga
     6001 agcagtaggt aaggagattc tcaaccacct catgcatgga gtccagaagc tttgtcagtt
     6061 ggtaaaaccg ttgccagttc tgactggagt tcccttccct tttgacgatg gcttttccta
     6121 gctctttgat ataagtcatt cgaatctcat caaataactc ttggctcttc agaccttcct
     6181 taggaactga ggagagaagc agtaaggttt tcatacagag atactcttca taggatacct
     6241 gcaatctttg taattcagag gagacaaaca gcatgtgttt acattggtca tacatgcagg
     6301 gtagagacat tctctgctca ttaataatca gatcaggagc aaagcagagc aggtttccac
     6361 ttgattgtct gtatgatctc caacccaggg caaatgccat gagaaacatc catgagtact
     6421 gtagcagggt catttggtca tccaggtgta agtttctgaa gcctggtatc gcctttgccc
     6481 atttcactgc tgcaatcact tgacgcccac ctaacatgtt gagtgtggtc ataattctcc
     6541 atgctgaatc tggaacagag ctatcatatc ctgcatacaa cacctcgggt tcaatcacct
     6601 ccagcagtga caccaaggta ggggtgagct gtggtaatgc tgcaggaact attgttttgt
     6661 taggattttc cgaagtgtct tgtgagactc ctgcagtggc ttgctgaatc cctttgattt
     6721 ttttctttgt ttttcgagct tcactagcca ttgtgttaac ttgtaaaccc taaccctaac
     6781 cccccacccc cacgttttga aattgaaaaa aacctctctc gaaagctttc tgatttcact
     6841 gaaaaacttc taatcccaaa gctccctcct ttaaccacca ccccaaatag ataaacccac
     6901 aagagaacta aaacagaaga aacaaaagga acttcctttc ttatgtttga agactgaaga
     6961 aaagaagagt cttgagagag aactcaagaa atgagagaag agaagaagaa gataagtaag
     7021 agaagaaaga gagagaagag acagaggttt aagagagttt tagacagata aggaaattag
     7081 accaagctgg ttcctttata cttatttcat gctgtgtgtt ttctgttcct attgttgaat
     7141 tattttttaa gaaaaagttg gtttttattt ttaaattttt cgaagggttt tgatctcatt
     7201 ataatgcttt tactttatga tgggtttctt tttggacaaa tgagcagctt ttgtatgctt
     7261 ttgatttttt gcatttcact caactttttt gtcttctctc ttttctttgt tcctctcctc
     7321 tcatttggtt ttacagtaac agtgcagctt cagtgtactc atcatatctc ttttaatttt
     7381 tggtcattat tagcgaataa gagcataaaa aaaagctgtc tttttttcaa atatatacta
     7441 atttacacat taataacttg tggagaaaaa atcgttttta aaatgcatgc taattgaatt
     7501 ggacatttgt aaaaagtaaa aatttattca tttcttggtt aagagaagtt ttgtctcttt
     7561 ttgcattgat gtgcgtagga gtgaccaaac aaagatcttc ttgatctttt tatgtatatc
     7621 ttaggttggt cctaaaaagg ttatttagac tcgcatttgt ttaatctcca ataaaggcat
     7681 atttttatcg aaaattatag tatcattttt attgtaatag tgaaaaccaa ttaattacaa
     7741 gaaggacaaa agagatgaat gagactctct ctctctctct ctcactcact cacgccaatc
     7801 ctcggagaga gacagcattg cattcccgga atactgcaaa agcaaccttc tgtctcatct
     7861 ctccctcctt ttgtttatac cacataatca tttattattt tatataaact tcaatcaatt
     7921 ttggtcttaa gtgaaaggta tttgtcaaaa aaaaactctt gtagctcctc tggtctgcta
     7981 aggctaagtg ctaacgtgtg acgagaacga tcctcaagaa ttgcataaag agaagaagta
     8041 aaggaagaag gaaaacaaaa tgaaatgtgt tcggtacaaa caaaaaaggg ttaagattta
     8101 tttttgtgac agtaatattt cattttctta ctcataccta ttaaattcaa tttaatgtaa
     8161 atcttagtta taaacttata ataatcaatt aataaattga aacttaattt ggtattgcaa
     8221 ataattactt aaattgtgca aaaatcacaa aaacatatag tacttaagaa acagagggaa
     8281 tatatttttg agttgtctga tacaaaaatt cccaacaatg ttatttacca taaaaatccc
     8341 aacaacccac tttaatactt ctttttcccc actaactaaa agaactataa aaagaagcaa
     8401 aaacttagaa tgtcgcccaa aattagcttt agaatcatct ttgttaatac atgtttcgaa
     8461 ctttgcatat tggtaatgac gaactaacta ctgtaactat aaatattttg tatgtttcca
     8521 aaaaattctt aatattgatt gatttcatga aataaagaaa ctttaaaaga ttttgattca
     8581 aaaagcggct aaggggacca agattaaaat ggttgcccgc ggatatcgct cgaggattgg
     8641 tctatttcaa gcaatctctt gcttgcccac attagatatg ttgcatgtga aaatctccca
     8701 ccaaacattc ttaaacgaat tgtattgtaa gtttgtaaca tatagatagt ggacaaaaac
     8761 gctctttgtt ttcattacaa gaacttacat tgaagtttta ctataaatac ttaaatagat
     8821 atagtcttgt ggttcatcat aaaattaagt tgatttaagt gaagaacaat caaatgcgaa
     8881 tgtaattact caaaaagcta gaaaattagt gattaatctt gaatttgaat agatagtaat
     8941 tggtttgatc atggtgttaa tggactcctt aaactcaaaa taatgtgttt ttctctcgac
     9001 tattgtgcaa gcacactatc gtccacttgc cactcttgct ttatttccca ataacacttc
     9061 tcatctatat aacggatctt attgaatctc ttttcggtca aaattcgaaa aagaaacttc
     9121 atggtaaaag ggctttatta tattctcttc atcaacatat atgtctttat ccatgtgtat
     9181 aattaagtaa tttagtttaa aaccacgtgg taaaaggact ttggtgttaa ccccaccatg
     9241 catttgttgt ttgaccgata aaagaactca cagcaaaaaa aatgaaacgg attaacaaga
     9301 tagaagatta gtgtttgctt tagattacgt cgtctcaaag acgtagtttt tcattacaaa
     9361 ttctatatgc tgcattttct atcgtcggat aactcttttt tttttccaac agctgtagtt
     9421 cttcattaca aactataatc ctaaaacaaa tgtgctatac tacgtaccat aagattagta
     9481 atagtatagt catcaatgcg gtatcggcat tgttcgtctg tggctaaatt tttgcaatgg
     9541 atatattcgt gaaaacattt gtagtagagg tcgaagcctc aatcaataaa ttcgaagtgg
     9601 aagatgtaaa aatgcaaata tataggtgct acgaatgaaa attgtcatat acttttgaga
     9661 tttttatcac tgcgacttga ataacatttt aactggtaac agtactgcac ttttaaaagg
     9721 tttagtggat aagtctgtgt tttatttttt ggaattttct gatcatcgaa aacaaatata
     9781 gcaaacaaaa atatacgctt taccactgca tgaagaaatc taatctatca taattctttg
     9841 ctaatacaga taatcaagac atctttgtga tatcatactc gatcttgttt ctaaccttcg
     9901 aagtcaaaaa tgtcgtctag agttgaaatt aattcatgcc aaaactaata cacatcatgg
     9961 gttgcttttg gctaaactat ttaattaatt tgtagattat ataccctcat gattaatagc
    10021 aaacacagaa aaatgtttaa cattaagaaa agaaaaaagg caaagagaaa gctaaagatt
    10081 ttggttctac atgatattag ctaatgaata ttggagcact actttgctct tagtttagtg
    10141 taatgatcat aacttctaac ttttgtgtaa tcaaagaagc caagatgggt cttattatgg
    10201 tggagagagt ccaaatcatt ggggaataat attcccatag gacccatgaa atgagagaaa
    10261 gaccaaatat aaatatattt aattgagact actctatttg caaggaaagt atatactttt
    10321 agagaaagat tgatgaagaa aaatgcaaat gaagttttct ttaatactga taaggagcta
    10381 aagtggcaaa gtttggagtt ctaatgattt ggtttgcatt ttgccttaca tatatccagc
    10441 aatagtttgg aatacaaaga tgaccccatc attgcaaatg catcttgagt ctatatatta
    10501 aaagatatat aagcaacttc atcgaaaacg gaaatctatt ctaagtttga gatcaatcaa
    10561 tagaatcgtt aagacttaaa gcccatgagg tgtcataatc gattcggtgt cttgatagtg
    10621 cttttgacca ttattgttaa tatttgatat ttggacagtt ataattaata ttgaacttgg
    10681 aaattttaaa gaagaaaata ataaactaca cctatttctt tttctacctt gtgaagtatt
    10741 tacaaactct gaaactgaaa ctacatcaaa catcgcaacg atgattgtcg atatattata
    10801 atcaataaga aagaaaaata tcagagtttg aaatttttta tttattttaa taacgagttt
    10861 gaaagttaaa aacacgttta ggtttagcta gcataagtat atgatcagac caataatgcc
    10921 aaataaaaac acgggtttga ttttttttgt ttggatatat tggcgagaaa attaacaaag
    10981 gaaaaggttt acaaaattct aaaataaaaa attgaaaaag gtgaaaatgc atgggagtgt
    11041 gaaaatgaaa gcacataact ttacgttcca tattatacgg tttccactaa tttgtttatc
    11101 gcattaacga ataaaaggcg aacctttaat tttctgcaat actactactt acttcttatc
    11161 agagcaacat atatacattt aaaaaatata tatatataca ttatgcaaga tactttttgc
    11221 ctataaatgt ttgtgctagt taatttttgt acttatttat ttaaattatt attatctggt
    11281 gcagtaaaaa ttcaacttta attatagtgt tgacacatca atacctatat taactttaaa
    11341 tgtggatgta acacatccct cttgttaaag gtatatcaac gcaaagggta gacctatata
    11401 ttgttctatg atatttacga atatggatct aaatcagtgt ctcgcaaact agtatagacg
    11461 ggccgattat gtattgtcat ttgtgataga acttacgtgc ttaatattac taatatatgt
    11521 atgtcatcgt gaaaaactta aaaagtattt atactttgtg ttacgtatcg ctgcgaaaaa
    11581 tatatgtacg cattaatttt gaggtttagt gtttataata ataaattatt caaaaagata
    11641 ttagctaggt atgagactgt agaatagata tgaaattaga aagaagaaaa aacgcatgtt
    11701 aattttatat ggaaaagggc tttttggtgt tcatcggatc taaaggggac aggagtgatc
    11761 attaaagtat taaaggagaa ggggaaaaaa aggtaaagag agaaggttaa atccaaggga
    11821 taaaaaaagg caaaaaaaaa aaaagagtgt taattatttt ctttaccttt ttttcaaatg
    11881 ttaatgcttt tcttcttttg catggaaaag ataaagaaac taatcatcat tactctctct
    11941 tttcttctct aatcccaatg aagcatgaat ctcgatcgat caagctttta tgaaaattga
    12001 gtctacaaag gaacccaccg gatcataaag agggtttgat cttttgagaa tagctgagat
    12061 acttgtaaga aatgactcag attttagtgt tcgggtttaa gtgtgggaga aactcggtga
    12121 aagagagggt tcgaagtctg ttgtgattca agcgtaaccc gtgaggcgag ctgactaaag
    12181 tcataacacg tgaggcgagc tgactaaagt cataacacgt gaggcgagct gactaaagtc
    12241 ataacaagct tgagggcgtt ttggtctttt cgcatgagct atgtaactat atcttcttct
    12301 tcttctctgt aattccgtta gatcgggata agagtttgtt gagatcaaag agagagagga
    12361 gtgtagtgag agaaagcttg aaagcattgt aacagagact aagtttccaa gttctagtgg
    12421 atttccggag aggtttctcc ggcgagacgt agcgttccgg tttggaacgt gaactcgtta
    12481 aattctctgt gttcagcttt tcttttactt tctttcagtt ctgatcaatt cgattcttag
    12541 tgctgaagtt gattcgtgtg tttgtgagat tgaagtgaag ttagatcttt acaaattggt
    12601 atcagagctc acggttgttg agcgagtgcg aatctaagag gtggtacctg gtgcgatcgc
    12661 tgttggcgcg ccgtgtttaa ttaaggttgc ggccgcttac ttcgtccgag cctagttcga
    12721 gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc
    12781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    12841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    12901 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12961 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    13021 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    13081 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    13141 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    13201 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    13261 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    13321 ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    13381 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    13441 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    13501 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    13561 tcaatctaaa gtatatatgt gtaacattgg tctagtgatt atttgccgac taccttggtg
    13621 atctcgcctt tcacgtagtg aacaaattct tccaactgat ctgcgcgcga ggccaagcga
    13681 tcttcttgtc caagataagc ctgcctagct tcaagtatga cgggctgata ctgggccggc
    13741 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
    13801 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
    13861 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
    13921 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
    13981 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
    14041 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
    14101 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
    14161 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
    14221 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
    14281 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
    14341 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcataac accccttgta
    14401 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
    14461 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
    14521 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
    14581 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
    14641 actttctggc tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag
    14701 cgggct
//
