LOCUS       sequence-227537-        7728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 683, 
            Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP24-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..7728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1512
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     protein_bind    3403..3414
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4477..4598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4514..4533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4568..4587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4605..5060)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4742..4763)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4954..4973
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5087..5191
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5193..5384
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5216..5236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5381..5515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5564..6358
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5564..6358
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5618..5637)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6228..6247
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6546..6565)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6590..6637
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6966..7554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7455..7474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgtcggt accgctgact acctcagcat tcggccacgc gtttctggct
      601 aactgtgaac gcgagcagat ccacctggcg ggctccattc agccgcacgg tatcctgctg
      661 gctgtgaaag agccggacaa cgtggtgatc caggcttcta ttaacgctgc ggagttcctg
      721 aacaccaact ctgttgttgg ccgtccgctg cgtgacctgg gcggcgatct gcctttgcag
      781 atcctgccgc acctgaacgg cccgctgcac ctggctccga tgaccctgcg ttgtaccgtg
      841 ggttctccgc cgcgtcgtgt ggactgtacc attcatcgtc cgtctaacgg cggcctgatc
      901 gtagaactgg aaccagcaac caagaccact aacattgcgc cggctctgga cggtgcgttt
      961 catcgtatca cttcttcatc ctccctgatg ggcctgtgtg acgaaaccgc gactattatc
     1021 cgtgagatta ctggctacga ccgtgtgatg gtagtacgtt tcgatgaaga gggtaatggc
     1081 gaaattctgt ccgaacgtcg tcgtgcggac ctggaagcgt tcctgggtaa ccgctacccg
     1141 gcgtctacta ttccgcagat cgctcgtcgc ctgtacgaac ataaccgtgt tcgcctgctg
     1201 gtagatgtga actatactcc ggttccgcta cagccgcgca tcagcccgct gaacggtcgt
     1261 gatctggata tgtccctgtc ttgcctgcgc tctatgtccc cgatccacca gaaatacatg
     1321 caggacatgg gcgttggcgc gaccctggtt tgctctctga tggtgtctgg tcgtctgtgg
     1381 ggtctgatcg cttgccacca ctacgaaccg cgcttcgttc cgttccacat tcgcgctgct
     1441 ggcgaagcgc tggcggaaac ttgtgcgatc cgcatcgcga cgctggagag ctttgcacag
     1501 tctcagtcca aaggtggcgg aagcggcggc ggaagcggtg gcggaagtgg tggcggaagc
     1561 ggcggcggaa gcggtggcgg aagttccgga ctcagatctg gcagcggtgg aggcttggag
     1621 gagccggagg ccgcgacgcg gacagccgcg gccgtggatt gcaaagacag acccgggttc
     1681 ccagtgaaga ggttaataca agcccgcttg cccttcaaac gcctgaacct tgttcccaag
     1741 gagaaagttg aggaagacac atcaccaaaa gcagctgtgg aaagcaaagt cccggatcta
     1801 caattgtctt tgggcacttt tgaaagccag tgtcacacag gctctcacgt aggcttgagt
     1861 acaaagttgg tcggtggcca gggccccatt gatagcttct tgagggcaac aatcaagcca
     1921 gtaccgagtg tggtcattat cgacctgacg gagaactgca gtgacattcc agacagccct
     1981 gagggccaca gtgaactaag tcctgacact gctggtgtgg tcaccacagt ggaaggagct
     2041 gccaagcagc aggagcactc tgcagcagag ctgtgtctcc tagagacccc ttcagacatc
     2101 acttgtcaca tggaagagga gcctgggagt ccaggagacc caaagaggac gggtgactgt
     2161 caggctggtt cactacagag ctgtccggag ctgaccccag gttctaggac atgccccacc
     2221 aaggagttat caagctggag caaggcaggg gatctcctat ttatagagaa agtgcctgtg
     2281 gtggtgctag aggacatcct ggccactaag ccctccattg cctctcttcc catgatgtcc
     2341 ctggatagga gtgtcacttc agagagtgaa atactggagt cctgccccga ggatgactcc
     2401 atactgagcc actcctccac caactcctct tctccaacca gctctcctga gggaccatcc
     2461 acacccccag aacatcgggg tgggagaagc tccccctcca ccccagcctg cagagttgcc
     2521 aagaactttg tcaaaggctc cacagagaag ggcaggagca aactgcacag agatagagaa
     2581 cagcagaggg aagagaagga aaagctgagg gaagagatac gacgagccaa ggaggaggca
     2641 cggaagaaga aggaggagga gaaggagctg aaggaaaagg agcggaggga aaagcgggag
     2701 aaagatgaga aggagaaggc ggagaagcag cggctaaagg aggaaaaacg caaggagcgg
     2761 caggaagctc tggaggctaa actggaggag aagaggaaga aggaggaaga gaagcggtta
     2821 cgggaggaag agaagcggtt acgggaagaa gagaagcgca ttaaggcaga aaaggcagag
     2881 atcactcgat tcttccagaa accgaagact ccgcaggctc ccaagaccct ggccggttcc
     2941 tgtggtaagt ttgccccctt tgaaatcaaa gaacacatgg tgctggctcc acggtgccga
     3001 gctgccttgg atcaagacct gtgcgatcag ctggaccagc tcctgcagca gcagagtgtg
     3061 gcaagcacct tcctcagtga cctaaaaagc cggctgccct tgagatcggg gcccacccgg
     3121 gtctgtggcc atgacacaga catcatgaac agggatgtgg tcattgtgga gagcagcaag
     3181 gtggacggtg tgtctgaaag gaagaagttt ggccgcatga agcttctgca gttctcagag
     3241 aaccaccggc cagcatactg ggggacgtgg aacaagaaga cagccatcat ccgcccccgg
     3301 aacccctggg cccaggacaa agaccttctt gactatgagg tagacagtga cgatgagtgg
     3361 gaagaggagg aacctgggga gtctttgtcc cacagtgagg gggatgagga tgacgatgtg
     3421 ggggaagatg aggatgagga cgatggcttc tttgtgcccc atgggtactt atctgaagat
     3481 gaaggcgtga ctgaggagtg tgcagaccct gagaaccaca aagtacacca gaagctgaag
     3541 gccaaggagt gggatgagct cctagccaag ggcaagcgct ttcgtgtgct gcagcctgtg
     3601 cacgtgggat gtgtatgggc atcagaggca gccaactgca cgagttccga cttgaagctg
     3661 ctgcagcagt tcactgcatg cctgctggat gtggcatccc ctgatgagcc agaacccggg
     3721 gcctccagga gggaaaagag ggaccagcat atcctggccc agttgcttcc gctgctacat
     3781 ggcaatgtga atgggagcaa ggtgatcatc catgaattcc aggagcaatg ccgcaggggc
     3841 ctgctgaccc ttccaagtcc caccccacac ctgcagatgc ccaacttgga ggatgctgtt
     3901 gctgtgccat ccaaggccag gctgaagcgc cttatctctg agaactctgc ctatgagaag
     3961 cggcccaact tccgtatgtg ctggtatgtg cacccagagg tgctcaagag ctttggccag
     4021 gagtgcctgc cagtgccctg ccagtggacc tacatcacca ccatgccctc agcacccagg
     4081 gaagacagtg gcagtgcttc tactgagggg cctggccaga gcacacctat gctactcaag
     4141 cggaagccag cggccaccat gtgcatcacg cagtttatga agaagcggcg ctatgatgga
     4201 caggttgggt ctggggacat ggatggcttc caggcagaca cagaagagga tgaagaggat
     4261 gacacagact gtatgatcat agatgtgcca gatgttggga gtgacgtgtc agaagctccc
     4321 atacctgctc ccacactttg caagtgagga tccaccggat ctagataact gatcataatc
     4381 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4441 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4561 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     4621 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4681 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4741 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4801 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4861 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4921 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4981 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5041 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5161 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     5221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5461 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5521 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5581 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5641 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5701 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5761 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5821 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5881 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5941 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6001 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6061 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6121 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6181 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6241 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6301 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6361 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6421 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6481 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6541 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6601 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6661 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6721 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6781 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6841 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6901 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6961 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7021 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7081 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7141 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7201 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7261 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7321 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7381 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7441 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7501 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7561 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7621 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     7681 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
