LOCUS       sequence-227550-       10634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to 
            firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, 
            Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, 
            Cravatt BF, Clardy J, Kaelin WG Jr
  TITLE     Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 
            Senses Cysteine.
  JOURNAL   Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042.
  PUBMED    27368101
REFERENCE   2  (bases 1 to 10634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-30- 
            30"; volume: "166"; issue: "1"; pages: "126-39"
COMMENT     Sequence Label: pLenti HRE mutant-Luc pGK Hygro
FEATURES             Location/Qualifiers
     source          1..10634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    337..454
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        798..860
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             924..2576
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(990..1008)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        2671..3170
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2706..2729)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3103..3122
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3188..4213
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3188..4213
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4244..4277)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4333..4921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4386..4406)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4804..4815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4924..4940)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4925..4941)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5130..5363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5435..5556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5472..5491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5526..5545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5596..5731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5751..5770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5752..5770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5793..6151)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5833..5854)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6045..6064
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6178..6282
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6284..6641
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6284..6475
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6307..6327)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6492..6627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6676..7470
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6676..7470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6730..6749)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7340..7359
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7658..7677)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7702..7749
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8078..8666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8567..8586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8841..9144
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9146..9344
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9299..9319
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9362..9542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9589..9714
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10211..10444
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(10629..10634,1..39)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
       61 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
      121 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
      181 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
      241 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
      301 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      361 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      421 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      481 tccagtttgg ttagtaccgg gcccgctcta gccctagatt tgggctcgaa gccctaccag
      541 ctgtctcaca cagcctgtct gacctctcga cctaccggcc gttcgaagcc ctaccagctg
      601 tctcacacag ccttctgatc tcgacctacc ggccgttcga agccctacca gctgtctcac
      661 acagcctgtc tgacctctcg acctaccggc cgttcgagtc gacagctgta ctgcagagat
      721 ccggccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg
      781 cgcggtccga ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga
      841 ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatctccaa gcttggcatt
      901 ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc
      961 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat
     1021 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt
     1081 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
     1141 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
     1201 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
     1261 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt
     1321 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg
     1381 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
     1441 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg
     1501 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata
     1561 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
     1621 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
     1681 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
     1741 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct
     1801 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
     1861 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc
     1921 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac
     1981 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
     2041 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga
     2101 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
     2161 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
     2221 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg
     2281 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc
     2341 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
     2401 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
     2461 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
     2521 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct
     2581 agactgccgg atcgaattgt cgacgaattc ggatccgcgg ccgcgtttaa acttaattaa
     2641 ggccggccct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     2701 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2761 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2821 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2881 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2941 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3001 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcnggctc aggggcgggc
     3061 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     3121 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc
     3181 cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     3241 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     3301 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     3361 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     3421 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     3481 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     3541 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     3601 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     3661 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     3721 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     3781 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     3841 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     3901 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     3961 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     4021 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     4081 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     4141 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     4201 ggcaaaggaa tagtgtacaa gtaggaattc gtcgagggac ctaataactt cgtatagcat
     4261 acattatacg aagttataca tgtttaaggg ttccggttcc actaggtaca attcgatatc
     4321 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4381 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4441 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4501 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4561 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4621 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4681 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4741 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4801 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4861 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4921 catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa
     4981 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     5041 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5101 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5161 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5341 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     5401 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     5461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     5581 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5641 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5701 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     5761 agtcgtatta gcatgtgagc aaaaggccag cataaatcaa aagaatagac cgagataggg
     5821 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     5881 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     5941 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6001 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6061 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     6121 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     6181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     6301 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6361 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6421 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6481 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6541 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     6661 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6721 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6781 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6841 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6901 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6961 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     7021 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     7081 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     7141 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     7201 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7261 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7321 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7381 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7441 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     7501 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7561 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7621 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     7681 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     7741 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     7801 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     7861 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     7921 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8761 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
     8821 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     8881 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     8941 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9001 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     9061 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     9121 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     9181 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     9241 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     9301 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
     9361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9541 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     9601 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     9661 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     9721 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     9781 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     9841 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     9901 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     9961 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    10021 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
    10081 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10141 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10201 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10261 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10321 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    10381 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    10441 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    10501 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
    10561 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    10621 agcaagaaaa gaat
//
