LOCUS       sequence-227551-        4238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant 
            fused to the GAL-4 DNA binding domain. This construct cannot undergo
            sumoylation on K294..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Hietakangas V, Anckar J, Blomster HA, Fujimoto M, Palvimo JJ, Nakai 
            A, Sistonen L
  TITLE     PDSM, a motif for phosphorylation-dependent SUMO modification.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 
            10.1073/pnas.0503698102. Epub 2005 Dec 21.
  PUBMED    16371476
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0503698102"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2006-01-3- 3"; volume: "103"; issue: "1"; pages: "45-50"
COMMENT     Sequence Label: Gal4-HSF4b K294R
FEATURES             Location/Qualifiers
     source          1..4238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..441
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    1466..1600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1503..1522)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1557..1576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1663..1682)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1947..1964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2118..2706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2198..2217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2877..3668,3669..3737))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3500..3519
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3738..3842)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3897..3917)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
       61 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
      121 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
      181 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt
      241 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
      301 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
      361 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
      421 caaagacagt tgactgtatc gccggaattc gggccgagca atgcaggagg caagagaaag
      481 ctgtccctga tgctggatga ggggagctca tgcccaacac ctgccaagtt caacacctgc
      541 cctctacctg gtgcccttct gcaggacccc tacttcatcc agtcgcctct cccagagaca
      601 aatttgggcc ttagccctca cagggccagg ggccccatca tctctgacat cccagaagac
      661 tctccatccc ctgaggggac caggctttct ccctccagtg atggcaggag ggagaagggc
      721 ctggcactgc tccgagaaga gccggccagt ccaggggggg atggcgaggc cgggctggcc
      781 ctggccccaa acgagtgtga cttctgcgtg acagcccccc cgccactgcc tgtggctgtg
      841 gtgcaggcca tcctggaagg gaaagggagc ttcagccccg atgggcccag gaatgcccaa
      901 cagcctgaac caggggatcc cagggagata cctgacaggg ggcctctggg cctggaaagc
      961 ggagacagga gcccagagag tctgctgcct ccgatgctgc ttcagccccc tcaagaaagt
     1021 gtggaacctg cagggcctct agatgtgctg ggccccagtc tccaagggcg agaatggacc
     1081 ctgatggact tggacatgga gctgtccttg atgcagccct tggttccaga gcggggtgag
     1141 cctgagctgg cggtcaaggg gttaaattct ccaagcccag ggaaggaccc cacgctcggg
     1201 gccccactcc tgctggatgt ccaggcggcc ttgggaggcc cagccctggg cctgcctggg
     1261 gctttaacca tttatagcac tcctgagagc cggactgcct cctacttggg cccggaagcc
     1321 agtccctccc cctaagaatt cgagctctag ataagtaatg atcataatca gccatatcac
     1381 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1441 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1501 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1561 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatcctctac gccggacgca
     1621 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     1681 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     1741 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     1801 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     1861 cgcataaggg agagcgtcga tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1921 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1981 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2521 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3001 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3061 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3181 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3241 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3301 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3361 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3421 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3481 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3541 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3601 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3661 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3721 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3781 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3841 cgcacatttc cccgaaaagt gccacctgac cttctgaggc ggaaagaacc agctgtggaa
     3901 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3961 catgcatctc aattagtcag caaggaaagt ccccaggctc cccagcaggc agaagtatgc
     4021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga agaggctttt
     4201 ttggaggaga tctaagcttg aagcaagcct cctgaaag
//
