LOCUS       sequence-227699-        8965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with a 
            N-terminal 3xFLAG-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, 
            Berger B, Gingras AC, Lindquist S
  TITLE     A quantitative chaperone interaction network reveals the 
            architecture of cellular protein homeostasis pathways.
  JOURNAL   Cell. 2014 Jul 17;158(2):434-48. doi: 10.1016/j.cell.2014.05.039.
  PUBMED    25036637
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "434-48"
COMMENT     Sequence Label: pLenti6.2-3xFLAG-V5-ccdB
FEATURES             Location/Qualifiers
     source          1..8965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1882..2185
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2186..2389
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2340..2360
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2386..2410
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2479..2544
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2563..2604
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2602..2726
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2751..2756,2757..2774,2775..2781)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2835..3494
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2901..2920)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3836..4141
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4020..4039
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4182..4306)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4348..4389
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4422..4921
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4457..4480)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4854..4873
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4935..4982
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5001..5399
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5486..5719
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5791..5912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5828..5847)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5882..5901
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5952..6087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6107..6126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6108..6126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6136..6153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6136..6152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6145..6167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6294..6749
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6381..6400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6591..6612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6775..6879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6880..6948,6949..7740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7098..7117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7911..8499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8400..8419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8653..8670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8787..8808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8823..8828,8829..8846,8847..8853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8861..8877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8866..8888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8885..8901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8885..8901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8920..8940
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8922..8940
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatg
     1801 tcgacgttaa cgctagcgac tgggtctgcg atgtcgacgt taacgctagc gactgggtct
     1861 gcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1921 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1981 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2041 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2101 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2161 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2221 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2281 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2341 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     2401 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
     2461 ctagaggatc cagccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
     2521 gactacaagg atgacgatga caagggctcg ggctcgacct cgggtaagcc tatccctaac
     2581 cctctcctcg gtctcgattc tacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     2641 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     2701 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
     2761 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     2821 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     2881 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     2941 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     3001 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     3061 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     3121 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     3181 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     3241 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     3301 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     3361 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     3421 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     3481 agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt
     3541 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     3601 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     3661 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     3721 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     3781 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     3841 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     3901 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     3961 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     4021 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     4081 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     4141 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     4201 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     4261 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgct ctatagtaac
     4321 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     4381 gattctacgc gtaccggtta gtaatgacga tggtacctac cgggtagggg aggcgctttt
     4441 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     4501 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     4561 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     4621 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     4681 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     4741 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     4801 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     4861 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     4921 gactctagac acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     4981 acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     5041 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     5101 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     5161 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     5221 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     5281 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     5341 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     5401 cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5461 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5521 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5581 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5641 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5701 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     5761 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     5821 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5881 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     5941 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6001 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6061 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     6121 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6181 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6241 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     6301 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6361 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6421 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6481 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6541 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6601 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6661 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6721 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     7141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7561 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8041 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8221 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8281 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8341 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8401 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8461 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8521 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8581 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8641 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8701 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8761 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8821 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8881 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     8941 gaacaaaagc tggagctgca agctt
//
