LOCUS       sequence-227705-        8234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ADAR2 expression plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
  TITLE     Differential Binding of Three Major Human ADAR Isoforms to Coding 
            and Long Non-Coding Transcripts.
  JOURNAL   Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 
            10.3390/genes8020068.
  PUBMED    28208661
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2017-02-11"; volume: "8(2). pii: genes8020068. 
            doi"; pages: " 10.3390/genes8020068"
COMMENT     Sequence Label: pmGFP-ADAR2
FEATURES             Location/Qualifiers
     source          1..8234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        104..483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        484..687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     638..658
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     732..751
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        732..750
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             778..807
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(817..819,820..822,823..1533)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(817..819,820..822,823..1533)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(862..883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1123..1142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1470..1491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3694..3711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3700..3924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3970..4398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4057..4076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4267..4288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4407..4427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4412..4741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4592..4727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4654..4673
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4808..5602
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4862..4881)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5472..5491
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5776..5897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5813..5832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5867..5886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5946..5962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5946..5962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5959..5981)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5970..5986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5994..6000,6001..6018,6019..6024))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6039..6060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6177..6194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6348..6936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6428..6447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7107..7898,7899..7967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7730..7749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7968..8072)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8147..8166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
       61 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      481 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      541 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      601 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      661 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
      721 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc caccatggaa
      781 caaaaactca tctcagaaga ggatctgtcc ggcggcatgg tgagcaaggg cgaggagctg
      841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1021 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1261 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1321 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1381 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1441 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1501 gggatcactc tcggcatgga cgagctgtac aagtccggcg gcgaattcat ggatatagaa
     1561 gatgaagaaa acatgagttc cagcagcact gatgtgaagg aaaaccgcaa tctggacaac
     1621 gtgtccccca aggatggcag cacacctggg cctggcgagg gctctcagct ctccaatggg
     1681 ggtggtggtg gccccggcag aaagcggccc ctggaggagg gcagcaatgg ccactccaag
     1741 taccgcctga agaaaaggag gaaaacacca gggcccgtcc tccccaagaa cgccctgatg
     1801 cagctgaatg agatcaagcc tggtttgcag tacacactcc tgtcccagac tgggcccgtg
     1861 cacgcgcctt tgtttgtcat gtctgtggag gtgaatggcc aggtttttga gggctctggt
     1921 cccacaaaga aaaaggcaaa actccatgct gctgagaagg ccttgaggtc tttcgttcag
     1981 tttcctaatg cctctgaggc ccacctggcc atggggagga ccctgtctgt caacacggac
     2041 ttcacatctg accaggccga cttccctgac acgctcttca atggttttga aactcctgac
     2101 aaggcggagc ctccctttta cgtgggctcc aatggggatg actccttcag ttccagcggg
     2161 gacctcagct tgtctgcttc cccggtgcct gccagcctag cccagcctcc tctccctgtc
     2221 ttaccaccat tcccaccccc gagtgggaag aatcccgtga tgatcttgaa cgaactgcgc
     2281 ccaggactca agtatgactt cctctccgag agcggggaga gccatgccaa gagcttcgtc
     2341 atgtctgtgg tcgtggatgg tcagttcttt gaaggctcgg ggagaaacaa gaagcttgcc
     2401 aaggcccggg ctgcgcagtc tgccctggcc gccattttta acttgcactt ggatcagacg
     2461 ccatctcgcc agcctattcc cagtgagggt cttcagctgc atttaccgca ggttttagct
     2521 gacgctgtct cacgcctggt cctgggtaag tttggtgacc tgaccgacaa cttctcctcc
     2581 cctcacgctc gcagaaaagt gctggctgga gtcgtcatga caacaggcac agatgttaaa
     2641 gatgccaagg tgataagtgt ttctacagga acaaaatgta ttaatggtga atacatgagt
     2701 gatcgtggcc ttgcattaaa tgactgccat gcagaaataa tatctcggag atccttgctc
     2761 agatttcttt atacacaact tgagctttac ttaaataaca aagatgatca aaaaagatcc
     2821 atctttcaga aatcagagcg aggggggttt aggctgaagg agaatgtcca gtttcatctg
     2881 tacatcagca cctctccctg tggagatgcc agaatcttct caccacatga gccaatcctg
     2941 gaagaaccag cagatagaca cccaaatcgt aaagcaagag gacagctacg gaccaaaata
     3001 gagtctggtg aggggacgat tccagtgcgc tccaatgcga gcatccaaac gtgggacggg
     3061 gtgctgcaag gggagcggct gctcaccatg tcctgcagtg acaagattgc acgctggaac
     3121 gtggtgggca tccagggatc cctgctcagc attttcgtgg agcccattta cttctcgagc
     3181 atcatcctgg gcagccttta ccacggggac cacctttcca gggccatgta ccagcggatc
     3241 tccaacatag aggacctgcc acctctctac accctcaaca agcctttgct cagtggcatc
     3301 agcaatgcag aagcacggca gccagggaag gcccccaact tcagtgtcaa ctggacggta
     3361 ggcgactccg ctattgaggt catcaacgcc acgactggga aggatgagct gggccgcgcg
     3421 tcccgcctgt gtaagcacgc gttgtactgt cgctggatgc gtgtgcacgg caaggttccc
     3481 tcccacttac tacgctccaa gattaccaaa cccaacgtgt accatgagtc caagctggcg
     3541 gcaaaggagt accaggccgc caaggcgcgt ctgttcacag ccttcatcaa ggcggggctg
     3601 ggggcctggg tggagaagcc caccgagcag gaccagttct cactcacgcc ctgagcggcc
     3661 gctctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3721 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3781 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3841 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3901 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3961 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4021 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4081 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4141 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4201 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4261 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4321 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4381 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4561 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4621 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4681 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4741 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     4801 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4861 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4921 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4981 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5041 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5101 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5161 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5221 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5281 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5341 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5401 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5461 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5521 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5581 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5641 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5701 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5761 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5821 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5881 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5941 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6001 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6061 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6121 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6181 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6241 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6361 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6421 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6481 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6541 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6601 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6661 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6721 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6781 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6841 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6901 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6961 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7021 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7081 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7141 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7201 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7261 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7321 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7381 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7441 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7501 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7561 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7621 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7681 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7741 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7801 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7861 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7921 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7981 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8041 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8101 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     8161 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     8221 gcgcgagcaa aatt
//
