LOCUS       sequence-227709-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-1 chain (Col1a1) with 
            mApple replacing exon 2-3 retaining N-propeptide minor triple helix 
            and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-1(I)
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1012..1713
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELY"
     primer_bind     1422..1441
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6091..6108)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6097..6321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6367..6795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6454..6473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6664..6685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6804..6824)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6809..7138
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6989..7124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7051..7070
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7205..7999
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7259..7278)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7869..7888
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8173..8294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8210..8229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8264..8283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8343..8359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8343..8359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8356..8378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8367..8383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8391..8397,8398..8415,8416..8421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8574..8591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8745..9330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8825..8844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9501..10292,10293..10361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10124..10143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10362..10466)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgt ctagggtcta gacatgttca gctttgtgga cctccggctc
      961 ctgctcctct taggggccac tgccctcctg acgcatggcc aagaagatat cgtgagcaag
     1021 ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1081 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1141 gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1201 atcctgtccc ctcagttcat gtacggctcc aaggtctaca ttaagcaccc agccgacatc
     1261 cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc
     1321 gaggacggcg gcattattca cgttaaccag gactcctccc tgcaggacgg cgtgttcatc
     1381 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1441 accatgggct gggaggcctc cgaggagcgg atgtaccccg aggacggcgc cctgaagagc
     1501 gagatcaaga agaggctgaa gctgaaggac ggcggccact acgccgccga ggtcaagacc
     1561 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
     1621 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1681 cgccactcca ccggcggcat ggacgagctg tacgagtcag aagatgtagg agtcgaggga
     1741 cccaagggag accctggccc ccaaggccca aggggacccg ttggcccccc tggacgagat
     1801 ggcatccctg gacagcctgg acttcctggt cctcctggtc cccctgggcc ccccggaccc
     1861 cctggccttg gaggaaactt tgcttcccag atgtcctatg gctatgatga aaaatcagct
     1921 ggagtttccg tgcctggccc catgggtcct tctggtcctc gtggtctccc tggcccccct
     1981 ggtgcacctg gtccacaagg tttccaaggc ccccctggtg aacctggcga gcctggcggt
     2041 tcaggtccaa tgggtccccg aggtccccct ggccctcctg gcaagaatgg agatgatggg
     2101 gaagctggca agcccggccg tcctggtgag cgtggacctc ctggacctca gggtgctcgt
     2161 ggattgcctg gaacagctgg cctccctgga atgaagggac accgaggctt cagtggtttg
     2221 gatggtgcca aaggagatgc tggtcctgct ggtcctaagg gagagcccgg cagtcctggt
     2281 gaaaacggag ctcctggcca gatgggtccc cgaggtctgc ccggtgagag aggtcgccct
     2341 ggacctcctg gcactgctgg tgctcgcggt aacgatggtg ctgttggtgc tgctggaccc
     2401 cctggtccca ccggccccac tggccctcct ggcttccctg gtgcagttgg tgctaagggt
     2461 gaagctggtc cccaaggagc tagaggctct gaaggtcccc agggtgtgcg tggtgagccc
     2521 ggaccccctg gccctgctgg tgctgccggc cctgctggaa accctggtgc tgatggacaa
     2581 cctggcgcta aaggtgccaa tggtgctcct ggtattgctg gtgctcctgg cttccctggt
     2641 gcccgaggcc cctctggacc ccagggcccc agcggccctc caggtcccaa gggtaacagt
     2701 ggtgaacctg gtgctcctgg caacaaagga gacactggtg ccaaaggaga acccggtgct
     2761 actggagttc aaggtccccc aggccctgcc ggagaagaag gaaaacgagg agcccgtggt
     2821 gagcctggac cttccggact gcctggacct cctggcgagc gtggtggacc tggtagccgt
     2881 ggtttccctg gtgctgatgg tgttgctggc cccaagggtc cttccggtga acgtggtgct
     2941 cccggacctg ctggtcccaa aggttctcct ggtgaagctg gtcgccccgg tgaagctggt
     3001 ctccctggtg ccaagggtct cactggcagt cctggcagcc ctggtcctga tggcaaaacc
     3061 ggcccccctg gtcccgctgg tcaagatggt cgccctggac ccgcaggtcc tcctggagcc
     3121 cgtggccagg ctggtgtgat gggattccct ggacctaagg gtaccgctgg agaacctgga
     3181 aaggctggag agcgaggcct tcccggaccc cctggcgctg ttggtcctgc tggcaaagat
     3241 ggagaagctg gagctcaggg agcccctggc cctgctggtc ctgctggtga gagaggtgaa
     3301 caaggtcccg ctggctcccc tggattccag ggtcttcctg gtcctgccgg tcctcctggt
     3361 gaagcaggca agcctggtga acagggtgtt cctggagacc ttggtgcccc tggaccctct
     3421 ggcgcaagag gcgagagagg tttccctggt gaacgtggtg ttcaaggtcc cccaggtcct
     3481 gctggtcccc gaggaaacaa tggtgccccc ggcaacgatg gtgccaaggg tgatactggt
     3541 gcccccggag ctcccggtag ccagggtgcc cccggtcttc agggaatgcc tggtgaacgt
     3601 ggtgcagctg gtcttccagg tcctaagggt gacagaggtg atgctggtcc caaaggtgct
     3661 gatggttctc ctggtaaaga tggtgcccgt ggtctgactg gtcccattgg tcctcctggc
     3721 cctgctggtg cccctggtga caagggtgaa gctggtccca gtggtcctcc cggtcccacc
     3781 ggagcccgtg gtgctcccgg agaccgtggt gaggctggtc cccctggtcc tgctggcttt
     3841 gccggccccc ctggtgctga tggccaacct ggtgcgaaag gtgaacctgg tgatactggt
     3901 gttaaaggtg atgctggtcc tcctggccct gctggtcctg ctggaccccc cggccccatt
     3961 ggtaacgttg gtgctcctgg acccaaaggt cctcgtggtg ctgctggtcc ccctggtgct
     4021 actggcttcc ctggtgctgc tggccgtgtc ggtccccctg gtccctctgg aaatgctgga
     4081 ccccctggcc ctcccggtcc cgttggcaaa gaagggggca aaggtccccg tggtgagact
     4141 ggccctgctg gacgtcctgg tgaagttggt cccccaggtc cccccggtcc tgctggtgag
     4201 aaaggatctc ctggtgctga tggacctgct ggctctcctg gtacccctgg acctcagggt
     4261 attgctggac aacgtggtgt ggtcggtctt cccggtcaga gaggagaaag aggcttccct
     4321 ggtcttcctg gcccctctgg tgaacctggc aaacaaggtc cttctggatc aagtggtgaa
     4381 cgcggtcccc ctggccccat ggggccccct ggattggctg gtccccctgg tgaatctgga
     4441 cgtgagggat cccctggtgc tgaaggctcc cctggaaggg atggtgctcc cggggccaag
     4501 ggtgaccgtg gtgagactgg ccctgctggc ccccctggtg cccctggtgc tcccggtgct
     4561 cccggccctg ttggtcccgc tggcaagaat ggcgatcgtg gtgagactgg tcctgctggt
     4621 cctgctggtc ccattggccc tgctggtgcc cgtggccctg ctggacccca aggcccccgt
     4681 ggtgacaagg gtgagacagg cgaacaaggt gacagaggca taaagggtca tcgtggcttc
     4741 tctggtctcc agggtcctcc tggttctcct ggttctcctg gtgaacaagg cccctctgga
     4801 gcttcaggtc ctgcaggccc ccggggtccc cctggctctg ctggttctcc tggcaaagac
     4861 ggactcaacg gtctccctgg ccccattggt ccccctggtc ctcgaggtcg cactggtgac
     4921 agcggccctg ctggtccccc cggccctcct ggaccccctg gccctcctgg acctcccagt
     4981 ggcggttatg acttcagctt cctgcctcag ccacctcaag agaagtctca agatggtggc
     5041 cgctactacc gggccgatga tgctaacgtg gttcgtgacc gtgaccttga ggtggacacc
     5101 accctcaaga gcctgagtca gcagattgag aacatccgca gccccgaagg cagccgcaag
     5161 aaccctgccc gcacatgccg cgacctcaag atgtgccact ctgactggaa gagcggagag
     5221 tactggatcg accctaacca aggctgcaac ctggacgcca tcaaggtcta ctgcaacatg
     5281 gagacaggtc agacctgtgt gttccctact cagccgtctg tgcctcagaa gaactggtac
     5341 atcagcccga accccaagga aaagaagcac gtctggtttg gagagagcat gaccgatgga
     5401 ttcccgttcg agtacggaag cgagggctcc gaccccgccg atgtcgctat ccagctgacc
     5461 ttcctgcgcc taatgtccac cgaggcctcc cagaacatca cctatcactg caagaacagc
     5521 gtagcctaca tggaccagca gactggcaac ctcaagaagg ccctgctcct ccagggatcc
     5581 aacgagatcg agctcagagg cgaaggcaac agtcgcttca cctacagcac ccttgtggac
     5641 ggctgcacga gtcacaccgg aacttggggc aagacagtca tcgaatacaa aaccaccaag
     5701 acctcccgcc tgcccatcat cgatgtggct cccttggaca ttggtgcccc agaccaggaa
     5761 ttcggactag acattggccc tgcctgcttc gtgtaaactc cctccacccc aatctggttc
     5821 cctcccaccc agcccacttt tccccaaccc tggaaacaga cgaacaaccc aaactcaatt
     5881 tcccccaaaa gccaaaaata tgggagataa tttcacatgg actttggaaa acattttttt
     5941 tcctttgcat tcacctttca aacttagttt ttacctttga ccaactgaac gtgaccaaaa
     6001 accaaaagtg cattcaacct taccaaaaaa gaaaaaaaaa aaaaaagcgg ccgctcgagt
     6061 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6121 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6181 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6241 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6301 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6361 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6421 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6481 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6541 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6601 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6661 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6721 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6781 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6841 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6961 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7141 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     7201 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7261 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7321 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7381 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7441 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7501 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7561 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7621 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7681 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     7741 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7801 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7861 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7921 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7981 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     8041 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8161 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag
     9301 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9781 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
