LOCUS       sequence-227710-       10506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-1 chain (Col1a1) with eGFP 
            replacing exon 2-3 retaining N-propeptide minor triple helix and 
            N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: eGFP-pro-alpha-1(I)
FEATURES             Location/Qualifiers
     source          1..10506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1012..1014,1015..1722)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(1012..1014,1015..1722)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(1054..1075)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1315..1334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1662..1683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6100..6117)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6106..6330
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6376..6804
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6463..6482)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6673..6694
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6813..6833)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6818..7147
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6998..7133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7060..7079
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7214..8008
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7268..7287)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7878..7897
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8182..8303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8219..8238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8273..8292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8352..8368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8352..8368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8365..8387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8376..8392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8400..8406,8407..8424,8425..8430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8445..8466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8583..8600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8754..9339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8834..8853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9510..10301,10302..10370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10133..10152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10371..10475)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgt ctagggtcta gacatgttca gctttgtgga cctccggctc
      961 ctgctcctct taggggccac tgccctcctg acgcatggcc aagaagatat cgtgagcaag
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acgagtcaga agatgtagga
     1741 gtcgagggac ccaagggaga ccctggcccc caaggcccaa ggggacccgt tggcccccct
     1801 ggacgagatg gcatccctgg acagcctgga cttcctggtc ctcctggtcc ccctgggccc
     1861 cccggacccc ctggccttgg aggaaacttt gcttcccaga tgtcctatgg ctatgatgaa
     1921 aaatcagctg gagtttccgt gcctggcccc atgggtcctt ctggtcctcg tggtctccct
     1981 ggcccccctg gtgcacctgg tccacaaggt ttccaaggcc cccctggtga acctggcgag
     2041 cctggcggtt caggtccaat gggtccccga ggtccccctg gccctcctgg caagaatgga
     2101 gatgatgggg aagctggcaa gcccggccgt cctggtgagc gtggacctcc tggacctcag
     2161 ggtgctcgtg gattgcctgg aacagctggc ctccctggaa tgaagggaca ccgaggcttc
     2221 agtggtttgg atggtgccaa aggagatgct ggtcctgctg gtcctaaggg agagcccggc
     2281 agtcctggtg aaaacggagc tcctggccag atgggtcccc gaggtctgcc cggtgagaga
     2341 ggtcgccctg gacctcctgg cactgctggt gctcgcggta acgatggtgc tgttggtgct
     2401 gctggacccc ctggtcccac cggccccact ggccctcctg gcttccctgg tgcagttggt
     2461 gctaagggtg aagctggtcc ccaaggagct agaggctctg aaggtcccca gggtgtgcgt
     2521 ggtgagcccg gaccccctgg ccctgctggt gctgccggcc ctgctggaaa ccctggtgct
     2581 gatggacaac ctggcgctaa aggtgccaat ggtgctcctg gtattgctgg tgctcctggc
     2641 ttccctggtg cccgaggccc ctctggaccc cagggcccca gcggccctcc aggtcccaag
     2701 ggtaacagtg gtgaacctgg tgctcctggc aacaaaggag acactggtgc caaaggagaa
     2761 cccggtgcta ctggagttca aggtccccca ggccctgccg gagaagaagg aaaacgagga
     2821 gcccgtggtg agcctggacc ttccggactg cctggacctc ctggcgagcg tggtggacct
     2881 ggtagccgtg gtttccctgg tgctgatggt gttgctggcc ccaagggtcc ttccggtgaa
     2941 cgtggtgctc ccggacctgc tggtcccaaa ggttctcctg gtgaagctgg tcgccccggt
     3001 gaagctggtc tccctggtgc caagggtctc actggcagtc ctggcagccc tggtcctgat
     3061 ggcaaaaccg gcccccctgg tcccgctggt caagatggtc gccctggacc cgcaggtcct
     3121 cctggagccc gtggccaggc tggtgtgatg ggattccctg gacctaaggg taccgctgga
     3181 gaacctggaa aggctggaga gcgaggcctt cccggacccc ctggcgctgt tggtcctgct
     3241 ggcaaagatg gagaagctgg agctcaggga gcccctggcc ctgctggtcc tgctggtgag
     3301 agaggtgaac aaggtcccgc tggctcccct ggattccagg gtcttcctgg tcctgccggt
     3361 cctcctggtg aagcaggcaa gcctggtgaa cagggtgttc ctggagacct tggtgcccct
     3421 ggaccctctg gcgcaagagg cgagagaggt ttccctggtg aacgtggtgt tcaaggtccc
     3481 ccaggtcctg ctggtccccg aggaaacaat ggtgcccccg gcaacgatgg tgccaagggt
     3541 gatactggtg cccccggagc tcccggtagc cagggtgccc ccggtcttca gggaatgcct
     3601 ggtgaacgtg gtgcagctgg tcttccaggt cctaagggtg acagaggtga tgctggtccc
     3661 aaaggtgctg atggttctcc tggtaaagat ggtgcccgtg gtctgactgg tcccattggt
     3721 cctcctggcc ctgctggtgc ccctggtgac aagggtgaag ctggtcccag tggtcctccc
     3781 ggtcccaccg gagcccgtgg tgctcccgga gaccgtggtg aggctggtcc ccctggtcct
     3841 gctggctttg ccggcccccc tggtgctgat ggccaacctg gtgcgaaagg tgaacctggt
     3901 gatactggtg ttaaaggtga tgctggtcct cctggccctg ctggtcctgc tggacccccc
     3961 ggccccattg gtaacgttgg tgctcctgga cccaaaggtc ctcgtggtgc tgctggtccc
     4021 cctggtgcta ctggcttccc tggtgctgct ggccgtgtcg gtccccctgg tccctctgga
     4081 aatgctggac cccctggccc tcccggtccc gttggcaaag aagggggcaa aggtccccgt
     4141 ggtgagactg gccctgctgg acgtcctggt gaagttggtc ccccaggtcc ccccggtcct
     4201 gctggtgaga aaggatctcc tggtgctgat ggacctgctg gctctcctgg tacccctgga
     4261 cctcagggta ttgctggaca acgtggtgtg gtcggtcttc ccggtcagag aggagaaaga
     4321 ggcttccctg gtcttcctgg cccctctggt gaacctggca aacaaggtcc ttctggatca
     4381 agtggtgaac gcggtccccc tggccccatg gggccccctg gattggctgg tccccctggt
     4441 gaatctggac gtgagggatc ccctggtgct gaaggctccc ctggaaggga tggtgctccc
     4501 ggggccaagg gtgaccgtgg tgagactggc cctgctggcc cccctggtgc ccctggtgct
     4561 cccggtgctc ccggccctgt tggtcccgct ggcaagaatg gcgatcgtgg tgagactggt
     4621 cctgctggtc ctgctggtcc cattggccct gctggtgccc gtggccctgc tggaccccaa
     4681 ggcccccgtg gtgacaaggg tgagacaggc gaacaaggtg acagaggcat aaagggtcat
     4741 cgtggcttct ctggtctcca gggtcctcct ggttctcctg gttctcctgg tgaacaaggc
     4801 ccctctggag cttcaggtcc tgcaggcccc cggggtcccc ctggctctgc tggttctcct
     4861 ggcaaagacg gactcaacgg tctccctggc cccattggtc cccctggtcc tcgaggtcgc
     4921 actggtgaca gcggccctgc tggtcccccc ggccctcctg gaccccctgg ccctcctgga
     4981 cctcccagtg gcggttatga cttcagcttc ctgcctcagc cacctcaaga gaagtctcaa
     5041 gatggtggcc gctactaccg ggccgatgat gctaacgtgg ttcgtgaccg tgaccttgag
     5101 gtggacacca ccctcaagag cctgagtcag cagattgaga acatccgcag ccccgaaggc
     5161 agccgcaaga accctgcccg cacatgccgc gacctcaaga tgtgccactc tgactggaag
     5221 agcggagagt actggatcga ccctaaccaa ggctgcaacc tggacgccat caaggtctac
     5281 tgcaacatgg agacaggtca gacctgtgtg ttccctactc agccgtctgt gcctcagaag
     5341 aactggtaca tcagcccgaa ccccaaggaa aagaagcacg tctggtttgg agagagcatg
     5401 accgatggat tcccgttcga gtacggaagc gagggctccg accccgccga tgtcgctatc
     5461 cagctgacct tcctgcgcct aatgtccacc gaggcctccc agaacatcac ctatcactgc
     5521 aagaacagcg tagcctacat ggaccagcag actggcaacc tcaagaaggc cctgctcctc
     5581 cagggatcca acgagatcga gctcagaggc gaaggcaaca gtcgcttcac ctacagcacc
     5641 cttgtggacg gctgcacgag tcacaccgga acttggggca agacagtcat cgaatacaaa
     5701 accaccaaga cctcccgcct gcccatcatc gatgtggctc ccttggacat tggtgcccca
     5761 gaccaggaat tcggactaga cattggccct gcctgcttcg tgtaaactcc ctccacccca
     5821 atctggttcc ctcccaccca gcccactttt ccccaaccct ggaaacagac gaacaaccca
     5881 aactcaattt cccccaaaag ccaaaaatat gggagataat ttcacatgga ctttggaaaa
     5941 catttttttt cctttgcatt cacctttcaa acttagtttt tacctttgac caactgaacg
     6001 tgaccaaaaa ccaaaagtgc attcaacctt accaaaaaag aaaaaaaaaa aaaaagcggc
     6061 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6121 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6181 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6241 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6301 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6361 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6421 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6481 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6541 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6601 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6661 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6721 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6781 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6901 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6961 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7021 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7081 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7141 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     7201 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7261 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7321 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7381 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7441 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7501 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7561 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7621 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7681 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7741 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7801 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7861 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7921 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7981 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     8041 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8101 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8161 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8221 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8281 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8341 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8401 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8461 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8521 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8641 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8941 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9181 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
     9301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9361 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10501 gacgtc
//
