LOCUS       sequence-227718-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            mApple between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-2(I)
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1039..1743
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1449..1468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5737..5754)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5743..5967
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6013..6441
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6100..6119)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6310..6331
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6450..6470)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6455..6784
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6635..6770
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6697..6716
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6851..7645
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6905..6924)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7515..7534
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7819..7940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7856..7875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7910..7929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7989..8005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7989..8005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8002..8024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8013..8029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8037..8043,8044..8061,8062..8067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8082..8103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8220..8237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8391..8976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8471..8490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9147..9938,9939..10007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9770..9789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10008..10112)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgta
      961 gacatgctca gctttgtgga tacgcggact ctgttgctgc ttgcagtaac ttcgtgccta
     1021 gcaacatgcc aagatatcgt gagcaagggc gaggagaata acatggccat catcaaggag
     1081 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     1141 ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa ggtgaccaag
     1201 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1261 gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt ccccgagggc
     1321 ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt taaccaggac
     1381 tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1441 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga ggagcggatg
     1501 taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct gaaggacggc
     1561 ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1621 ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc
     1681 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1741 aaggatatct attctgacaa aggagtttca tctggccctg gaccaatggg tttaatggga
     1801 cccagaggcc ctcctggtgc tgttggagcc cctggccctc aaggtttcca aggacctgct
     1861 ggtgaacctg gtgagcctgg tcaaacgggt cctgcaggtc cccgtggtcc agctggctct
     1921 cctggcaagg ctggtgagga tggtcaccct ggaaaacccg gaagacctgg ggagagagga
     1981 gtcgttggac cacagggtgc tcgtggtttc cccgggactc ctggacttcc tggcttcaaa
     2041 ggcgtgaaag gacacagtgg tatggatgga ttaaagggac agcctggtgc acagggtgtg
     2101 aagggtgaac ccggtgcccc tggagagaat ggaactccag gtcaagcagg agcccgaggg
     2161 cttcctggtg agagagggcg tgtcggagct cctggtccag ctggtgcccg aggcagtgat
     2221 ggaagcgttg gccccgtcgg ccctgctggt cccattgggt ctgctggacc ccctggtttc
     2281 ccaggtgctc ctggtcccaa gggtgaactt gggcccgttg gtaaccctgg ccctgctggt
     2341 cctgctggtc cccgtggtga agtgggtctt ccaggtcttt ctggccctgt tggacctcct
     2401 ggcaaccctg gaacaaatgg gctcactggc gccaagggtg ctactggact ccctggtgtc
     2461 gctggggctc ccggtctccc tggcccccgt ggtattcctg gccccgctgg tgctgctggt
     2521 gctacaggtg ccagaggact tgttggtgag cctggtcctg ctggctccaa aggagaatcc
     2581 ggtaacaagg gtgagcctgg ctctgttgga gcccaaggtc ctcctggtcc cagcggtgaa
     2641 gaaggaaaga gagggtctcc cggagaagct ggatctgctg gccctgcagg gcctccaggg
     2701 cttagaggca gccctggttc tcgaggtctt cctggagctg atggcagagc tggtgtaatg
     2761 ggtcctcctg gcaatcgtgg ttcaaccggc cctgctggaa tccgaggtcc taatggagat
     2821 gccggtcgac ctggggaacc tggtctcatg ggacccagag gtcttcctgg gtctcctgga
     2881 aatgttggcc catctggtaa agaaggccct gtgggtctcc ctggcattga tggcagacct
     2941 ggcccaatcg gcccagctgg accaagaggt gaagctggca acattggatt ccctggaccc
     3001 aaaggcccct ctggtgatcc tggcaaacct ggtgagagag gacaccccgg tcttgctggt
     3061 gctcggggag ctccaggacc cgatggcaac aatggagctc agggcccccc tggaccccag
     3121 ggtgttcaag gtggcaaagg tgaacaaggc cctgctggtc ctcctggctt ccagggtctc
     3181 cctggtccct caggtactac tggagaagtt ggcaagcccg gagaaagggg tcttcctggt
     3241 gaattcggtc tccctggtcc tgctggtcca agaggagaac gtggtacccc gggtgagagt
     3301 ggagctgctg gcccttctgg tcctattgga agccgaggtc ccagtggagc cccagggcct
     3361 gatggaaaca agggtgaagc tggtgcagtc ggtgctccag gcagtgctgg tgcctctggg
     3421 cctggtgggc ttccaggaga gaggggtgct gctggcatac ctgggggcaa aggagaaaag
     3481 ggtgaaactg gtctccgagg tgacactggc aacactggta gagatggtgc tcgtggcatt
     3541 cctggtgctg taggtgcccc tggtcctgct ggggcctcag gtgaccgggg tgaagctggt
     3601 gctgccggtc cttctggccc agctggtcct cggggtagcc ctggtgaacg tggtgaagtt
     3661 ggccctgctg gccccaatgg atttgctggt cctgctggtg ctgctggcca acccggtgct
     3721 aaaggagaga agggaaccaa agggcctaag ggtgaaaatg gcattgttgg tccaaccggg
     3781 tctgttggag ctgctggccc atctggtcct aatggcccac ctggtcctgt tggaagtcgt
     3841 ggtgatggag ggccccctgg tatgactggc ttccctggtg ctgctggacg gactggtccc
     3901 cccggaccca gtggtattgc tggcccccct ggcccccctg gtgctgctgg aaaggaaggg
     3961 attcgtggtc cccgtggcga ccaaggtcca gttggccgaa ctggagaaac aggtgcatct
     4021 ggaccccctg gatttgttgg tgaaaagggt ccctctggag aacctggtac cgcaggagcc
     4081 cctggtaccg caggtcctca gggtcttctt ggtgctcccg gtattctggg tctccctggc
     4141 tctagaggtg aacgtggtct gccaggtatt gctggtgctc tgggtgaacc tggtcctctg
     4201 ggcatctcag gccctcctgg tgcccgtggt ccccctggtg ctgtgggcag ccctggagtt
     4261 aatggtgccc ctggtgaagc tggtcgtgat ggtaaccctg gcagcgatgg tcccccaggt
     4321 cgtgatggtc agcctggaca caagggagag cgtggttacc ctggcagcat tggtccgact
     4381 ggtgctgcag gtgcccctgg tcctcacggt tctgtgggtc ctgctgggaa acatggaaac
     4441 cgaggtgaac ctggtcctgc cggttctgtt ggtcctgttg gtgctgttgg tccaagaggt
     4501 cctagtggtc ctcaaggcat ccgaggtgac aaaggagaac ctggtgataa agggcacagg
     4561 ggtcttcctg gcttaaaagg atacagtgga ttgcagggtc ttcctggtct tgctggccta
     4621 catggtgacc aaggagctcc tggtcctgtg ggtcctgctg gtcccagggg tcctgctggg
     4681 ccttctggac ccgttggcaa agatggtaga tctgggcaac ctggcccagt cgggcctgct
     4741 ggtgttcgtg gttctcaggg tagccaaggt cccgctggtc cccctggtcc ccctggacct
     4801 cctggtcctc ctggtgtcag cggaggtggc tatgactttg gttttgaagg agacttctac
     4861 agggctgacc agcctcgctc ccagccttca ctcagaccca aggactatga agttgatgca
     4921 actctgaaat ctctcaataa ccagattgag acccttctca ctcctgaagg ctctagaaag
     4981 aaccctgctc gcacgtgccg ggacttaaga ctcagccacc cagagtggaa cagcgattac
     5041 tactggattg accctaacca aggatgtact atggatgcca tcaaagtgta ctgtgatttc
     5101 tctactggtg aaacctgcat ccaggcccaa cctgtaaaca ccccagcgaa gaactcatac
     5161 agccgcgccc aggccaacaa gcatgtctgg ttaggagaga ctatcaatgg tggcagccag
     5221 tttgaataca acgtagaagg ggtgtcctcc aaggaaatgg caactcagct cgccttcatg
     5281 cgcctgctag ccaaccgtgc ttctcagaac atcacctacc actgcaagaa cagcattgcg
     5341 tacctggatg aggagacggg cagcttgaac aaggctgtgc ttctgcaggg ttccaacgat
     5401 gttgaacttg ttgctgaggg caacagcagg ttcacctact ctgtcctagt cgatggctgc
     5461 tccaaaaaga caaatgaatg gggcaagaca atcattgaat acaaaacaaa taagccatct
     5521 cgcctgccgt tccttgacat tgcacctctg gacattggtg gtgctgacca agaattccgt
     5581 gtggaggttg gccccgtctg tttcaaataa gtgaactcaa cataaattaa aaaccaaaaa
     5641 cccctgaaaa aactttctct ttgccgtttc ctcctttaaa aaaaaaaaaa aagcggccgc
     5701 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     5761 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5821 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5881 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5941 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6001 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6061 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6121 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6181 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6241 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6301 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6361 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6421 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6541 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6601 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6661 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6721 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6781 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     6841 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6901 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6961 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7021 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7081 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7141 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7201 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7261 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7321 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7381 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7441 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7501 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7561 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7621 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     7681 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7741 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7801 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7921 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7981 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8041 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8101 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc
     8941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10141 gtc
//
