LOCUS       sequence-227722-       10153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            Gly610Cys mutation and mVenus between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mVenus-pro-alpha-2(I)-G610C
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1039..1041,1042..1752)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1081..1102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1342..1361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5747..5764)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5753..5977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6023..6451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6110..6129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6320..6341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6460..6480)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6465..6794
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6645..6780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6707..6726
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6861..7655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6915..6934)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7525..7544
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7829..7950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7866..7885)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7920..7939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7999..8015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7999..8015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8012..8034)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8023..8039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8047..8053,8054..8071,8072..8077))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8092..8113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8230..8247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8401..8986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8481..8500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9157..9948,9949..10017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9780..9799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10018..10122)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaagccgcc
      961 accatgctca gctttgtgga tacgcggact ctgttgctgc ttgcagtaac ttcgtgccta
     1021 gcaacatgcc aagatatcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1081 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1141 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     1201 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     1261 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1321 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1381 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1441 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     1501 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     1561 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1621 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag
     1681 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1741 gagctgtaca aggatatcta ttctgacaaa ggagtttcat ctggccctgg accaatgggt
     1801 ttaatgggac ccagaggccc tcctggtgct gttggagccc ctggccctca aggtttccaa
     1861 ggacctgctg gtgaacctgg tgagcctggt caaacgggtc ctgcaggtcc ccgtggtcca
     1921 gctggctctc ctggcaaggc tggtgaggat ggtcaccctg gaaaacccgg aagacctggg
     1981 gagagaggag tcgttggacc acagggtgct cgtggtttcc ccgggactcc tggacttcct
     2041 ggcttcaaag gcgtgaaagg acacagtggt atggatggat taaagggaca gcctggtgca
     2101 cagggtgtga agggtgaacc cggtgcccct ggagagaatg gaactccagg tcaagcagga
     2161 gcccgagggc ttcctggtga gagagggcgt gtcggagctc ctggtccagc tggtgcccga
     2221 ggcagtgatg gaagcgttgg ccccgtcggc cctgctggtc ccattgggtc tgctggaccc
     2281 cctggtttcc caggtgctcc tggtcccaag ggtgaacttg ggcccgttgg taaccctggc
     2341 cctgctggtc ctgctggtcc ccgtggtgaa gtgggtcttc caggtctttc tggccctgtt
     2401 ggacctcctg gcaaccctgg aacaaatggg ctcactggcg ccaagggtgc tactggactc
     2461 cctggtgtcg ctggggctcc cggtctccct ggcccccgtg gtattcctgg ccccgctggt
     2521 gctgctggtg ctacaggtgc cagaggactt gttggtgagc ctggtcctgc tggctccaaa
     2581 ggagaatccg gtaacaaggg tgagcctggc tctgttggag cccaaggtcc tcctggtccc
     2641 agcggtgaag aaggaaagag agggtctccc ggagaagctg gatctgctgg ccctgcaggg
     2701 cctccagggc ttagaggcag ccctggttct cgaggtcttc ctggagctga tggcagagct
     2761 ggtgtaatgg gtcctcctgg caatcgtggt tcaaccggcc ctgctggaat ccgaggtcct
     2821 aatggagatg ccggtcgacc tggggaacct ggtctcatgg gacccagagg tcttcctggg
     2881 tctcctggaa atgttggccc atctggtaaa gaaggccctg tgggtctccc tggcattgat
     2941 ggcagacctg gcccaatcgg cccagctgga ccaagaggtg aagctggcaa cattggattc
     3001 cctggaccca aaggcccctc tggtgatcct ggcaaacctg gtgagagagg acaccccggt
     3061 cttgctggtg ctcggggagc tccaggaccc gatggcaaca atggagctca gggcccccct
     3121 ggaccccagg gtgttcaagg tggcaaaggt gaacaaggcc ctgctggtcc tcctggcttc
     3181 cagggtctcc ctggtccctc aggtactact ggagaagttg gcaagcccgg agaaaggggt
     3241 cttcctggtg aattcggtct ccctggtcct gctggtccaa gaggagaacg tggtaccccg
     3301 ggtgagagtg gagctgctgg cccttctggt cctattggaa gccgaggtcc cagtggagcc
     3361 ccagggcctg atggaaacaa gggtgaagct ggtgcagtcg gtgctccagg cagtgctggt
     3421 gcctctgggc ctggtgggct tccaggagag aggggtgctg ctggcatacc tgggggcaaa
     3481 ggagaaaagg gtgaaactgg tctccgaggt gacactggca acactggtag agatggtgct
     3541 cgtggcattc ctggtgctgt aggtgcccct ggtcctgctg gggcctcagg tgaccggggt
     3601 gaagctggtg ctgcctgtcc ttctggccca gctggtcctc ggggtagccc tggtgaacgt
     3661 ggtgaagttg gccctgctgg ccccaatgga tttgctggtc ctgctggtgc tgctggccaa
     3721 cccggtgcta aaggagagaa gggaaccaaa gggcctaagg gtgaaaatgg cattgttggt
     3781 ccaaccgggt ctgttggagc tgctggccca tctggtccta atggcccacc tggtcctgtt
     3841 ggaagtcgtg gtgatggagg gccccctggt atgactggct tccctggtgc tgctggacgg
     3901 actggtcccc ccggacccag tggtattgct ggcccccctg gcccccctgg tgctgctgga
     3961 aaggaaggga ttcgtggtcc ccgtggcgac caaggtccag ttggccgaac tggagaaaca
     4021 ggtgcatctg gaccccctgg atttgttggt gaaaagggtc cctctggaga acctggtacc
     4081 gcaggagccc ctggtaccgc aggtcctcag ggtcttcttg gtgctcccgg tattctgggt
     4141 ctccctggct ctagaggtga acgtggtctg ccaggtattg ctggtgctct gggtgaacct
     4201 ggtcctctgg gcatctcagg ccctcctggt gcccgtggtc cccctggtgc tgtgggcagc
     4261 cctggagtta atggtgcccc tggtgaagct ggtcgtgatg gtaaccctgg cagcgatggt
     4321 cccccaggtc gtgatggtca gcctggacac aagggagagc gtggttaccc tggcagcatt
     4381 ggtccgactg gtgctgcagg tgcccctggt cctcacggtt ctgtgggtcc tgctgggaaa
     4441 catggaaacc gaggtgaacc tggtcctgcc ggttctgttg gtcctgttgg tgctgttggt
     4501 ccaagaggtc ctagtggtcc tcaaggcatc cgaggtgaca aaggagaacc tggtgataaa
     4561 gggcacaggg gtcttcctgg cttaaaagga tacagtggat tgcagggtct tcctggtctt
     4621 gctggcctac atggtgacca aggagctcct ggtcctgtgg gtcctgctgg tcccaggggt
     4681 cctgctgggc cttctggacc cgttggcaaa gatggtagat ctgggcaacc tggcccagtc
     4741 gggcctgctg gtgttcgtgg ttctcagggt agccaaggtc ccgctggtcc ccctggtccc
     4801 cctggacctc ctggtcctcc tggtgtcagc ggaggtggct atgactttgg ttttgaagga
     4861 gacttctaca gggctgacca gcctcgctcc cagccttcac tcagacccaa ggactatgaa
     4921 gttgatgcaa ctctgaaatc tctcaataac cagattgaga cccttctcac tcctgaaggc
     4981 tctagaaaga accctgctcg cacgtgccgg gacttaagac tcagccaccc agagtggaac
     5041 agcgattact actggattga ccctaaccaa ggatgtacta tggatgccat caaagtgtac
     5101 tgtgatttct ctactggtga aacctgcatc caggcccaac ctgtaaacac cccagcgaag
     5161 aactcataca gccgcgccca ggccaacaag catgtctggt taggagagac tatcaatggt
     5221 ggcagccagt ttgaatacaa cgtagaaggg gtgtcctcca aggaaatggc aactcagctc
     5281 gccttcatgc gcctgctagc caaccgtgct tctcagaaca tcacctacca ctgcaagaac
     5341 agcattgcgt acctggatga ggagacgggc agcttgaaca aggctgtgct tctgcagggt
     5401 tccaacgatg ttgaacttgt tgctgagggc aacagcaggt tcacctactc tgtcctagtc
     5461 gatggctgct ccaaaaagac aaatgaatgg ggcaagacaa tcattgaata caaaacaaat
     5521 aagccatctc gcctgccgtt ccttgacatt gcacctctgg acattggtgg tgctgaccaa
     5581 gaattccgtg tggaggttgg ccccgtctgt ttcaaataag tgaactcaac ataaattaaa
     5641 aaccaaaaac ccctgaaaaa actttctctt tgccgtttcc tcctttaaaa aaaaaaaaaa
     5701 gggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     5761 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5821 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5881 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5941 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6001 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6061 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6121 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6181 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6241 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6301 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6361 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6421 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6541 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6601 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6661 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6721 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6781 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     6841 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6901 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6961 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7021 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7081 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7141 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7201 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7261 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     7321 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7381 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7441 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7501 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7561 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7621 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     7681 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     7741 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7801 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7861 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7921 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7981 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8041 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8101 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8161 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8221 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8281 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8341 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8401 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8461 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8521 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8581 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8641 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8701 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8761 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8821 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8881 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     8941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9121 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9181 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9241 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9301 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9361 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9421 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9481 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9541 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9601 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9661 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9721 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9781 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9841 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9901 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9961 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10021 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10081 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10141 gccacctgac gtc
//
