LOCUS       sequence-227729-       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with GFP 
            between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: eGFP-pro-alpha-2(I)
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1039..1041,1042..1752)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1039..1041,1042..1752)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1081..1102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1342..1361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5746..5763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5752..5976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6022..6450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6109..6128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6319..6340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6459..6479)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6464..6793
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6644..6779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6706..6725
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6860..7654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6914..6933)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7524..7543
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7828..7949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7865..7884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7919..7938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7998..8014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7998..8014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8011..8033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8022..8038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8046..8052,8053..8070,8071..8076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8091..8112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8229..8246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8400..8985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8480..8499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9156..9947,9948..10016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9779..9798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10017..10121)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgta
      961 gacatgctca gctttgtgga tacgcggact ctgttgctgc ttgcagtaac ttcgtgccta
     1021 gcaacatgcc aagatatcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1081 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1141 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1201 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1261 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1321 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1381 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1441 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1501 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1561 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1621 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1681 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1741 gagctgtaca aggatatcta ttctgacaaa ggagtttcat ctggccctgg accaatgggt
     1801 ttaatgggac ccagaggccc tcctggtgct gttggagccc ctggccctca aggtttccaa
     1861 ggacctgctg gtgaacctgg tgagcctggt caaacgggtc ctgcaggtcc ccgtggtcca
     1921 gctggctctc ctggcaaggc tggtgaggat ggtcaccctg gaaaacccgg aagacctggg
     1981 gagagaggag tcgttggacc acagggtgct cgtggtttcc ccgggactcc tggacttcct
     2041 ggcttcaaag gcgtgaaagg acacagtggt atggatggat taaagggaca gcctggtgca
     2101 cagggtgtga agggtgaacc cggtgcccct ggagagaatg gaactccagg tcaagcagga
     2161 gcccgagggc ttcctggtga gagagggcgt gtcggagctc ctggtccagc tggtgcccga
     2221 ggcagtgatg gaagcgttgg ccccgtcggc cctgctggtc ccattgggtc tgctggaccc
     2281 cctggtttcc caggtgctcc tggtcccaag ggtgaacttg ggcccgttgg taaccctggc
     2341 cctgctggtc ctgctggtcc ccgtggtgaa gtgggtcttc caggtctttc tggccctgtt
     2401 ggacctcctg gcaaccctgg aacaaatggg ctcactggcg ccaagggtgc tactggactc
     2461 cctggtgtcg ctggggctcc cggtctccct ggcccccgtg gtattcctgg ccccgctggt
     2521 gctgctggtg ctacaggtgc cagaggactt gttggtgagc ctggtcctgc tggctccaaa
     2581 ggagaatccg gtaacaaggg tgagcctggc tctgttggag cccaaggtcc tcctggtccc
     2641 agcggtgaag aaggaaagag agggtctccc ggagaagctg gatctgctgg ccctgcaggg
     2701 cctccagggc ttagaggcag ccctggttct cgaggtcttc ctggagctga tggcagagct
     2761 ggtgtaatgg gtcctcctgg caatcgtggt tcaaccggcc ctgctggaat ccgaggtcct
     2821 aatggagatg ccggtcgacc tggggaacct ggtctcatgg gacccagagg tcttcctggg
     2881 tctcctggaa atgttggccc atctggtaaa gaaggccctg tgggtctccc tggcattgat
     2941 ggcagacctg gcccaatcgg cccagctgga ccaagaggtg aagctggcaa cattggattc
     3001 cctggaccca aaggcccctc tggtgatcct ggcaaacctg gtgagagagg acaccccggt
     3061 cttgctggtg ctcggggagc tccaggaccc gatggcaaca atggagctca gggcccccct
     3121 ggaccccagg gtgttcaagg tggcaaaggt gaacaaggcc ctgctggtcc tcctggcttc
     3181 cagggtctcc ctggtccctc aggtactact ggagaagttg gcaagcccgg agaaaggggt
     3241 cttcctggtg aattcggtct ccctggtcct gctggtccaa gaggagaacg tggtaccccg
     3301 ggtgagagtg gagctgctgg cccttctggt cctattggaa gccgaggtcc cagtggagcc
     3361 ccagggcctg atggaaacaa gggtgaagct ggtgcagtcg gtgctccagg cagtgctggt
     3421 gcctctgggc ctggtgggct tccaggagag aggggtgctg ctggcatacc tgggggcaaa
     3481 ggagaaaagg gtgaaactgg tctccgaggt gacactggca acactggtag agatggtgct
     3541 cgtggcattc ctggtgctgt aggtgcccct ggtcctgctg gggcctcagg tgaccggggt
     3601 gaagctggtg ctgccggtcc ttctggccca gctggtcctc ggggtagccc tggtgaacgt
     3661 ggtgaagttg gccctgctgg ccccaatgga tttgctggtc ctgctggtgc tgctggccaa
     3721 cccggtgcta aaggagagaa gggaaccaaa gggcctaagg gtgaaaatgg cattgttggt
     3781 ccaaccgggt ctgttggagc tgctggccca tctggtccta atggcccacc tggtcctgtt
     3841 ggaagtcgtg gtgatggagg gccccctggt atgactggct tccctggtgc tgctggacgg
     3901 actggtcccc ccggacccag tggtattgct ggcccccctg gcccccctgg tgctgctgga
     3961 aaggaaggga ttcgtggtcc ccgtggcgac caaggtccag ttggccgaac tggagaaaca
     4021 ggtgcatctg gaccccctgg atttgttggt gaaaagggtc cctctggaga acctggtacc
     4081 gcaggagccc ctggtaccgc aggtcctcag ggtcttcttg gtgctcccgg tattctgggt
     4141 ctccctggct ctagaggtga acgtggtctg ccaggtattg ctggtgctct gggtgaacct
     4201 ggtcctctgg gcatctcagg ccctcctggt gcccgtggtc cccctggtgc tgtgggcagc
     4261 cctggagtta atggtgcccc tggtgaagct ggtcgtgatg gtaaccctgg cagcgatggt
     4321 cccccaggtc gtgatggtca gcctggacac aagggagagc gtggttaccc tggcagcatt
     4381 ggtccgactg gtgctgcagg tgcccctggt cctcacggtt ctgtgggtcc tgctgggaaa
     4441 catggaaacc gaggtgaacc tggtcctgcc ggttctgttg gtcctgttgg tgctgttggt
     4501 ccaagaggtc ctagtggtcc tcaaggcatc cgaggtgaca aaggagaacc tggtgataaa
     4561 gggcacaggg gtcttcctgg cttaaaagga tacagtggat tgcagggtct tcctggtctt
     4621 gctggcctac atggtgacca aggagctcct ggtcctgtgg gtcctgctgg tcccaggggt
     4681 cctgctgggc cttctggacc cgttggcaaa gatggtagat ctgggcaacc tggcccagtc
     4741 gggcctgctg gtgttcgtgg ttctcagggt agccaaggtc ccgctggtcc ccctggtccc
     4801 cctggacctc ctggtcctcc tggtgtcagc ggaggtggct atgactttgg ttttgaagga
     4861 gacttctaca gggctgacca gcctcgctcc cagccttcac tcagacccaa ggactatgaa
     4921 gttgatgcaa ctctgaaatc tctcaataac cagattgaga cccttctcac tcctgaaggc
     4981 tctagaaaga accctgctcg cacgtgccgg gacttaagac tcagccaccc agagtggaac
     5041 agcgattact actggattga ccctaaccaa ggatgtacta tggatgccat caaagtgtac
     5101 tgtgatttct ctactggtga aacctgcatc caggcccaac ctgtaaacac cccagcgaag
     5161 aactcataca gccgcgccca ggccaacaag catgtctggt taggagagac tatcaatggt
     5221 ggcagccagt ttgaatacaa cgtagaaggg gtgtcctcca aggaaatggc aactcagctc
     5281 gccttcatgc gcctgctagc caaccgtgct tctcagaaca tcacctacca ctgcaagaac
     5341 agcattgcgt acctggatga ggagacgggc agcttgaaca aggctgtgct tctgcagggt
     5401 tccaacgatg ttgaacttgt tgctgagggc aacagcaggt tcacctactc tgtcctagtc
     5461 gatggctgct ccaaaaagac aaatgaatgg ggcaagacaa tcattgaata caaaacaaat
     5521 aagccatctc gcctgccgtt ccttgacatt gcacctctgg acattggtgg tgctgaccaa
     5581 gaattccgtg tggaggttgg ccccgtctgt ttcaaataag tgaactcaac ataaattaaa
     5641 aaccaaaaac ccctgaaaaa actttctctt tgccgtttcc tcctttaaaa aaaaaaaaaa
     5701 agcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     5761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5941 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6001 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6421 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     6481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6541 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6601 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6661 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6721 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6781 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     6841 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6901 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6961 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7021 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7081 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7141 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7201 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7261 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7321 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7381 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7441 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7501 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7561 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7621 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     7681 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     7741 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7801 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7921 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7981 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8041 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8101 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8161 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
     8941 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9001 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9061 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9121 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9181 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9241 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9301 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9361 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9421 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9481 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9541 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9601 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9661 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9721 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9781 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9841 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9901 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9961 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10021 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10081 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10141 ccacctgacg tc
//
