LOCUS       sequence-22773-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Stavropoulos TA, Strathdee CA
  TITLE     Synergy between tetA and rpsL provides high-stringency positive and 
            negative selection in bacterial artificial chromosome vectors.
  JOURNAL   Genomics. 2001 Feb 15. 72(1):99-104.
  PUBMED    11247671
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genomics. 
            2001 Feb 15. 72(1):99-104."
COMMENT     Sequence Label: pBAC-RT
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(683..702)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cnnntnangc cgcaaacgtg gcgtatgtac tcgtgtatat actaccactc ctaaaaaacc
       61 gaactccgcg ctgcgtaaag tatgccgtgt tcgtctgact aacggtttcg aagtgacttc
      121 ctacatcggt ggtgaaggtc acaacctgca ggagcactcc gtgatcctga tccgtggcgg
      181 tcgtgttaaa gacctcccgg gtgttcgtta ccacaccgta cgtggtgcgc ttgactgctc
      241 cggcgttaaa gaccgtaagc aggctcgttc caagtatggc gtgaagcgtc ctaaggctta
      301 atggttctcc aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
      361 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
      421 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
      481 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
      541 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
      601 gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc
      661 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcnggct
      721 cnccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      781 gggggantgt nggcncnatc tcctngcatg caca
//
